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In order to explore the influence of the thickness of Ni-P transition layer on the preparation of Ni-P-PTFE coating on stainless steel surface. Ni /N-P /Ni-P-PTFE three-layers structure gradient coating was prepared on stainless steel by electroplating combined with electroless plating. Scanning electron microscopy (SEM), X-ray diffraction (XRD), nano indentation, friction and wear tester, scratch tester and contact angle tester were used to characterize and test the microstructure, mechanical properties, adhesion and anti-adhesion property of the gradient coating. The results showed that, with the increased of N-P transition layer deposition time, the transition layer gradually thickened, the PTFE content in N-P-PTFE first decreased and then increased, the hardness and adhesion of N-PTFE coating first increased and then decreased. The maximum values of hardness and adhesion reach to 4.18 GPa and 13.49 N respectively when the N-P deposition time was 20 min. When the N-P deposition time was 25 min,the friction coefficient reached the minimum value of 0.17, and the contact angle between the surface of Ni-P-PTFE coating with water-based and oil-based liquid reached the maximum value of 101.1° and 60.06° respectively. At this time, the Ni-P-PTFE coating showed excellent adhesion resistance. [ABSTRACT FROM AUTHOR]