1. Effect of hsa_circ_0001573 on biological behaviors of breast cancer cells and its molecular mechanism
- Author
-
CHEN Hong, CHEN Junxia
- Subjects
circrna ,breast cancer ,hsa_circ_0001573 ,cell proliferation ,apoptosis ,xenograft tumors ,gnb4 ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
Background and purpose: Circular RNA (CircRNA) is a type of non-coding RNA with a closed circular structure, which has important potential in gene expression regulation. CircRNA is closely related to the occurrence and development of tumor. This study aimed to explore the influence of hsa_circ_0001573 on the biological behavior of breast cancer cells and its mechanism. Methods: Breast cancer tissues and paracancerous tissues of 4 patients surgically removed at the First Affiliated Hospital of Chongqing Medical University from January 2020 to January 2021 were collected, sequenced and analyzed by RNA microarray analysis. Real-time fluorescence quantitative polymerase chain reaction (RTFQ-PCR) was used to detect the relative expression of hsa_circ_0001573 in breast cancer tissues and paracancerous tissues of 40 cases, as well as normal human breast cancer epithelial cell MCF-10A and breast cancer cells (MCF-7 and SK-BR-3), and the location and expression were further examined by fluorescence in situ hybridization (FISH) assay. The interference vector was transfected into breast cancer cells. Cell counting kit-8 (CCK-8), EdU, wound healing, clonal formation, Hoechst 33342, TUNEL, flow cytometry and transwell assays were adopted to determine cell migration, invasion, proliferation and apoptosis. Western blot was employed to measure the expressions of CCND1, CCND2 and CDK4. The effect of hsa_circ_0001573 on the growth of xenograft tumors was observed in nude mice. RNA pulldown assay was performed to identify hsa_circ_0001573-associated proteins. Subcellular localization of hsa_circ_0001573 and GNB4 was clarified by FISH and immunofluorescence (FISH-IF), to explore potential molecular mechanisms. Results: The expression level of hsa_circ_0001573 was high in breast cancer (P
- Published
- 2023
- Full Text
- View/download PDF