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Objective·To study the effect of rhubarb administration on the balance between intestinal flora and the body.Methods·Wistar rats were randomly divided into 4 groups (n=8), which were given extractions of rhubarb 0.1 g/kg (low dose group), 2.5 g/kg (medium dose group), 4.5 g/kg (high dose group) and normal saline (control group) by intragastric administration for 5 d, and the daily change of fecal water content of rats was observed. Gas chromatography/time of flight mass spectrometry (GC/TOFMS) approach was used to detect the metabolites in serum, colon tissue and fecal of rats on the 5th day of administration. Principal component analysis (PCA) and partial least squares discrimination analysis (PLS-DA) were used to analyze the differences of metabolites between different dose groups and the control group. The metabolites with statistical significance were obtained by t-test.Results·The water content of rat feces in the dose group gradually increased with the time and dose after rhubarb administration. Compared with the control group, 28, 18 and 20 differential metabolites were obtained in serum, colon tissue and fecal samples from different dose groups, which showed significant changes (P

Objective During object tracking, motion information can predict the location of the object. If motion information is ignored or motion is inaccurately modeled, then tracking may fail. To deal with this issue, we introduce visual saliency, which can quickly capture the interesting object, in tracking. Furthermore, we propose a tracking algorithm based on spatio-temporal motion saliency. Method First, we propose a bottom-up computational model for spatio-temporal motion saliency according to the hierarchical motion processing in the visual cortex. We adopt 3D spatio-temporal filters for the coding of underlying motion signals and max-pooling operation for the coding of local features. Considering the temporal relationship between the spatio-temporal motion features in the historical and current frames, we construct the spatio-temporal motion saliency map by measuring the difference between consecutive frames. Second, in the frame of particle filter, we measure the correlation between the predictive state and the observation by combining spatio-temporal motion saliency with color histogram. The object state can then be determined and tracked. Result Compared with other methods, our approach can stably track the objects under unfavorable situations, such as variable lighting, background clutter, motion blurs, occlusion, and deformation. We can improve the tracking performance in terms of central position error, precision, and success rate. In addition, we integrate the spatio-temporal motion saliency into other tracking methods and achieve better results, which demonstrates the effectiveness of the spatio-temporal motion saliency for object tracking. Conclusion The spatio-temporal motion saliency can improve tracking performance as it measures motion information effectively, thereby enhancing the salient area and suppressing interference. [ABSTRACT FROM AUTHOR]

Most salt lakes of Chaidamu circular economy pilot area in Qinghai Province are chloride-type lakes, and many chlorine-contained by-products will be formed during the developing process. How to utilize and transform these by-products has an important significance for the chlorine balance in industry chain of salt lake area. The industrial metabolism process of chlorine element is analyzed from the main planed products in Chaidamu circular economy pilot area master plan. Two main problems that affect the utilization of chlorine element are chlorine element balance and potential pollution to the salt lake caused by calcium chloride. Three transforming and converting approaches are put forward, which are immobilization process that converts chlorine to solid products, transformation process that transforms chlorine to organic products and clean storage of calcium chlorine. If the above approaches can be put into practice, the balance of chlorine element will be achieved in the pilot area. [ABSTRACT FROM AUTHOR]

Objective: To investigate the combined effect of evodiamine (EVO) and norcantharidin (NCTD) on HepG2 cells., Methods: HepG2 cells were treated with EVO or/and NCTD at different concentrations in vitro, with blank culture medium as the negative control. The inhibitory effect of EVO or/and NCTD was determined by MTT assay. Cell cycle and cell apoptosis were assessed by flow cytometry (FCM) combined with PI staining and annexin V-FITC/PI, respectively. The expressions of Bax and Bcl-2 were examined by Western blotting. RESULTS MTT assay showed that NCTD and EVO had restraining effect on the growth of HepG2 cells. The combination of NCTD and EVO had significant synergistic inhibition effect (CI<1). The arrest rate of G2/M phase was (36.13±1.63)% in the EVO induced group and (10.67±0.89)% in the NCTD induced group, and the combination of EVO and NCTD up-regulated the rate to (73.42±1.92)% (P<0.05). In addition, the apoptosis rate of HepG2 cells treated by two-drug combination increased significantly from (15.78 ± 3.08)% in the EVO group and (11.47 ± 1.60)% in the NCTD group to (21.86±2.70)% in the EVO combined with NCTD group (P<0.05). Western blotting demonstrated that the expression level of Bax protein went up significantly (P<0.05), meanwhile Bcl-2 protein dropped significantly (P<0.05) in the combined induced group., Conclusion: EVO combined with NCTD showed synergetic effect on anti-proliferation and pro-apoptosis in HepG2 cells.