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Objective To investigate the underlying mechanism by which hypoxia caused sperm developmental dysfunction. Methods Mouse spermatocytes GC-2 were conventionally sub-cultured in 6-well plates and grew to the logarithmic growth stage. 8 cell culture plates were selected and randomly divided into the hypoxia group and the control group. The cells in the hypoxia group and the control group were cultured in a Inviv O2 work station in 1% 02, 5% C02 and 94% N2 at 37 ℃ and a constant temperature and humidity of 5% carbon dioxide incubator at 37 ℃, respectively. After hypoxia treatment for 6, 12, 24 and 48 h, one cell culture plate was randomly taken from the Inviv 02 work station and the control from the carbon dioxide incubator. The cells in the hypoxia group and the control group were all randomly divided into two parts, of which one was used to extract total RNA and the other to extract total protein. The expressions of HSPA2, HSF1 and HSF2 in GC-2 were detected by real-time quantitative PCR and western blot. Results A transient increase in HSPA2 mRNA levels was found in GC-2 cells under hypoxia treatment for 24 h (P <0. 05), but no significant changes were observed at the other time points (6, 12 and 48 h) (P > 0. 05). No significant change in HSPA2 protein was found in GC-2 cells under hypoxia treatment for 6 h (P〉0. 05), but significant decreases at the other time points (12, 24 and 48 h) were observed (P <0. 05). HSF1 mRNA expressions were significantly decreased in GC-2 cells under hypoxia treatment for 6 and 12 h (P <0. 05), and no obvious change at the time point of 24 h was observed (P05), but a significant increase was detected at the time point of 48 h (P < 0. 05). HSF1 protein levels were all significantly downregulated under hypoxia treatment at four time points (6, 12, 24 and 48 h) (P <0. 05). HSF2 mRNA expressions had no significant changes in GC-2 cells at all four time points (6, 12, 24 and 48 h) (P >0. 05), and HSF2 protein levels also showed no significant changes under hypoxia treatment at three time points (6, 12 and 24 h) (P〉0.05), but a significant decrease occurred at the time point of 48 h (P < 0. 05). Conclusion HSPA2, HSF1 and HSF2 expressions in GC-2 cells are all significantly changed under hypoxia, and they may participate in hypoxia-caused sperm developmental dysfunction. [ABSTRACT FROM AUTHOR]

[ Objective] This paper aimed to understand the biological information and function of the Heat shock transcription factor( Hsf) in apple. [ Method]36 Hsfs were retrieved from the apple genome GDH13 vl. l,confirmed by Pfam and NCBI Conserved Domain Database,and then,TBTools,ExPASy,MEME and other software were used to further analyze33 genes with complete Hsf domain. [ Result]The proteins encoded by these genes contained 189 - 530 amino acids, with molecular weights of 21 703. 71 - 58 972. 96, and isoelectric points of 4. 55 - 8. 58. The prediction of subcellular localization showed that MD00G1095900 and MD02G1171800 could be located in chloroplast/nucleus, MD05G1313700 could be located in nucleus/mitochondria,and other Hsf were localized in the nucleus. Chromosome mapping and synteny analysis showed that there was no tandem duplication of apple Hif genes, but there were segmental duplication in 16 pairs of 24 apple Hif genes. Under saline-alkali stress, the expression of most Hif genes were significantly down-regulated. Protein interaction network analysis showed that MD03 G1258300 interacts with heat shock protein, superoxide dismutase copper chaperone, protein phosphatase 2C, and sucrose unfermentable 1-associated protein kinase 'Y regulatory subunit. MD15G1283700 interacts with heat shock protein, IAA amino acid hydrolase and alkaline leucine zipper. [Conclusion] Hsfs of apple may play an important role in regulating the response to saline-alkali stress, which provides some theoretical reference for further study of biological functions of Hsfs in apple. [ABSTRACT FROM AUTHOR]

Heat shock transcription factor (beat sheek transcription Eacher, HSF) is an important stress regulator in plants. Many studies have shown that HSF can improve the ability of plants to adapt to stress by regulating the stress-related genes downstream of the signaling pathway, such as improving the tolerance of plants in drought and oxidative damage. The function in the post-storage. In this study, the cassava variety SC124 was used as the material, and a sphincter (SF family gene, named MISF10) was cloned from the wood layer leaf by RT-PCR technology. The results showed that: (1) The full length of the gene is 1098 p․ 365 amino acid residues, the relative molecular weight of the protein is 40.71D. The theoretical isoelectric point is 8.15. The heavy smoke location of the protein is predicted to be the nucleus of the smoke, and the protein sequence analysis results show that MISF10 has the highest similarity with Jatropha JHSF and Oak extract HHSF, Respectively, 80.31% and 0.54%. The protein sequence of the MSF10 gene is contained in the conserved domain of the IISF protein family, such as DBD.HR A Care.HBL-B Core, the common nuclear localization signal (mour healitin signal, NIS) of intake sequence, Indicating that the protein encoded by the MISF10 gene stems from the H5FC family members. It is not expressed in all tissues, and the expression is highest in leaves. estil) and light response (keepensive min) (4) The expression analysis results are also listed. Due to drought and BA treatment, I visited China. The MISF10 gene was released during the unblocked postharvest physiological changes. Please express the online results. This indicates that the MSPTO gene may be involved in ABBA-mediated postharvest physiological changes in the ABBA-mediated crown metaphor and wooden blocks. This lays the foundation for further research on its function in wood boarding and harvesting. [ABSTRACT FROM AUTHOR]

[Objective] Heat Shock Transcription Factor in a key regulatory factor in defense from abiotic stresses in planta. [ Method) In this reasarch, a HSF gene designated MeHSF18 was isolated from cassava leaven through RT-PCR method. The physicochemical properties, protein domain, phylogenetic tree, and expression profiles of MeHSF18 were analyzed. (Result) The full-length cDNA of MeHSF18 Was 867 hp, encoded a polypeptide of 289 amino acid residues with a predicted relative molecular mass of 31.67 kDa and an isoelectric point of 5.90. Multiple alignment showed that MeHSF18 shared a significant degree of sequence identify with other HSF proteins in Hevea brusiliensis and Jatropha curcas. The expression profile of 11 cassava tissue showed that the high expression of MeHSF18 wan in root. The transcriptome data results indicated that MeHSF18 was upregulated by drought stress and ABA treament. The expression of MeHSF18 was also induced in cassava PPD process. [Conclusion] These results suggested that MelHSF18 might be involved in ABA mediated drought stress response and participated in PPD process. [ABSTRACT FROM AUTHOR]