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Objective To investigate the labial alveolar bone thickness of maxillary incisors and mesiodistal space in the maxillary adjacent central and lateral incisors based on cone beam computed tomography(CBCT) image data, and provide reference for aesthetic implantation in cases of consecutive missing maxillary incisors. Methods CBCT image data of 619 patients were selected in this study.The labial alveolar bone thickness of maxillary incisors as well as the mesiodistal space in the maxillary adjacent central and lateral incisors were measured at three millimeters apical to the cementoenamel junctio, followed by statistical analysis. Results The labial alveolar bone thickness at the maxillary central incisor and lateral incisor locations was (1.03±0.32)mm and (0.96±0.36)mm, respectively, with central incisors having greater thickness than lateral incisors. The mesiodistal space in the maxillary adjacent central and lateral incisors was (15.94±1.18)mm, with males (16.31±1.13)mm exhibiting slightly greater space than females (15.59±1.12)mm, showing no left-right positional differences. Among these, 5.01% failed to satisfy a space of 14 mm. Conclusion The majority of maxillary incisors fail to meet the ideal thickness requirements of labial alveolar bone for implantation, necessitating bone augmentation surgery. For the maxillary adjacent central and lateral incisors, approximately 5.01% of the mesiodistal spaces fail to meet the essential horizontal distance for the placement of two implants. In such situations after tooth loss, it is recommended to place only one implant in the region of the central incisor and aprosthetic restoration consisting of an implant crown on this implant connected with a cantilever at the position of the lateral incisor for long-term aesthetic results.

Objective·To construct an mRNA vaccine encoding hemagglutinin (HA) of influenza A H1N1 virus, and explore the protective effects of different booster vaccination strategies.Methods·Firefly luciferase (Fluc) was used as the reporter gene to construct Fluc mRNA vaccine enveloped in lipid nanoparticles (LNP). The in vivo expression of Fluc mRNA-LNP after intramuscular injection was determined by live imaging assay in mice. Furthermore, M15-HA mRNA-LNP derived from H1N1 subtype (A/Michigan/45/2015) was constructed. Mice were immunized with 20, 10, 5, or 1 µg doses of M15-HA mRNA-LNP twice (with an interval of 3 weeks) through intramuscular injection. Serum antibody titers were measured by enzyme-linked immunosorbent assay (ELISA) at 2 weeks and 4 weeks after the second immunization, and functional antibody levels were detected by hemagglutination inhibition test. The third booster vaccination was performed 40 d after the second immunization in 1 µg dose group with 1 µg M15-HA mRNA-LNP or 10 µg HA subunit vaccine. The levels of specific antibody and functional antibody were detected by ELISA and hemagglutination inhibition test, respectively 2 weeks and 4 weeks later.Results·Live imaging assay showed that luciferase activity could be detected in mice 1 d after injection of Fluc mRNA-LNP. At 2 weeks and 4 weeks after the second immunization of M15-HA mRNA-LNP, HA-specific antibodies were significantly higher than those before the immunization in all vaccination groups at different doses (P=0.000). The hemagglutination inhibition test showed that the levels of functional antibodies in the 20 μg dose and 10 μg dose groups were significantly higher than those in the PBS control group (P

Nanomaterials have been widely used in many fields for their antibacterial and antiseptic effects, but their antibacterial properties cannot be brought into full play due to the aggregation of particles. The composite of nanoparticles and polymer materials can prevent the aggregation of nanoparticles to a certain extent, so turning nanomaterials into polymer composites for the applications in the field of antisepsis and preservation of agricultural products will have a broad prospect. In this study, pathogenic fungi were isolated from Dongzao jujube by the tissue isolation method, and their pathogenicity was verified by the Koch's law. The compound preservative was prepared by mixing nano-TiO2 with the substrate, sodium carboxymethyl cellulose. The plate bacteriostasis experiment was carried out on the isolated pathogenic bacteria, and the inhibition mechanism was preliminarily explored. The results showed that the isolated Dongzao jujube fruit rot was Fusarium sp. In vitro experiments showed that the nano-TiO2 compound preservative solution inhibited the growth of Fusarium sp. The minimum inhibitory concentration and minimum bactericidal concentration on pathogenic spores were 9.77x10-3 g/L and 39.06x10-3 g/L respectively. The nano-TiO2 preservative solution inhibited the growth of pathogen mainly by destroying the fungal cell membrane; the research on the preservation of Dongzhao jujube confirmed that the preservative solution can significantly reduce the rate of fruit rot and delay the reduction of the Vc content in the fruit. In conclusion, the composite solution of nano-TiO2 and CMC-Na can be used as an effective antibacterial means to control Fusarium in the postharvest jujube fruits, which provides effective technical support for prolonging the shelf-life of dongzao jujube. [ABSTRACT FROM AUTHOR]

;In order to explore the design and weaving of dragonfly pattern in small jacquard fabric, the design points were analyzed in this paper, dragonfly patterned jacquard fabric was designed with three design methods; horizontal jump, plain ground and warp rise, and dobby CAD software was used to simulate the fabric, and the simulation effect was analyzed. The results show that the pattern layout of the dragonfly pattern is symmetrical, the pattern is clear, the yarn tension is even, but the pattern size is small. The pattern contrast of designed dragonfly pattern plain jacquard fabric is outstanding, the fabric surface will present a rich visual effect when colored yarn with obvious contrast. The small jacquard fabric of dragonfly design has symmetrical layout, clear pattern, vivid wave, large size and relief effect. [ABSTRACT FROM AUTHOR]

Objective: To analyze the clinical phenotype and genetic characteristics of a Chinese pedigree affected with Hereditary antithrombin deficiency., Methods: A pedigree diagnosed at the the Second Affiliated Hospital of Wenzhou Medical University, Yuying Children's Hospital in June, 2020 was selected as the study subject. Plasma prothrombin time (PT), activated partial thromboplastin time (APTT), fibrinogen (FIB), and thrombin time (TT) of the probands and their pedigree members were determined using a STA-R automatic coagulation analyzer. Antithrombin activity (AT: A) and antithrombin antigen (AT: Ag) in plasma were determined with chromogenic substrate and immunonephelometry assays. All exons and flanking sequences of the anticoagulant protein gene SERPINC1 were amplified by PCR and subjected to Sanger sequencing. Candidate variants were verified with bioinformatic tools (PolyPhen-2, SIFT, Mutation Taster and PYMOL) to explore their effect on the function and structural conformation of the protein., Results: The probands (II-2, II-10), their brother (II-5) and sons (III-1, III-8) had shown normal PT, APTT, FIB, and TT, but significantly decreased AT: A and AT: Ag, with their levels being 34%, 57%, 56%, 48%, 53% and 13.51 mg/dL, 13.44 mg/dL, 18.39 mg/dL, 17.36 mg/dL, 17.71 mg/dL, respectively. The remaining pedigree members had normal values. Sanger sequencing revealed that the probands and all affected pedigree members had harbored a heterozygous c.851T>C (p.Met284Thr) missense variant in exon 5 of the SERPINC1 gene. Bioinformatic analysis and simulation suggested that the variant has resulted in alteration of hydrogen bonds at the c.851 position, which may affect the structure of the protein. Based on the guidelines from the American College of Medical Genetics and Genomics (ACMG), the variant was classified as pathogenic (PS1+PM1+PM5+PP1+PP4)., Conclusion: The probands and other affected members were all diagnosed with type I hereditary AT deficiency, for which the c.851T>C (p.Met284Thr) variant of the SERPINC1 gene may be accountable.

Objective To explore the regulatory axis of circular RNA Cbl proto-oncogene B (circCBLB)/miR-486-5p on the proliferation, apoptosis, and inflammatory cytokines of fibroblast-like synoviocytes in rheumatoid arthritis (RA-FLS). Methods Human RA-FLS were stimulated with 100 μL of 10 ng/mL of tumor necrosis factor-alpha (TNF-α) to establish the model. The binding relationship of circCBLB/miR-486-5p was validated by a dual-luciferase reporter gene assay. pcDNA3.1/siRNA-circCBLB, negative control (pcDNA3.1-NC/si-NC), and miR-486-5p-mimics were created and transfected into RA-FLS, respectively. The experiment was divided into seven groups: control, TNF-α-treated RA-FLS, pcDNA3.1-circCBLB, pcDNA3.1-NC, si-circCBLB, si-NC, and pcDNA3.1-circCBLB combined with miR-486-5p-mimics. Cell viability was assessed by a CCK-8 assay; cell cycle and apoptosis by flow cytometry; colony formation ability by a colony formation assay; and the expression levels of circCBLB and miR-486-5p by real-time quantitative PCR. The levels of interleukin 4 (IL-4), IL-10, IL-6 and TNF-α were measured by ELISA. Results The dual-luciferase reporter gene assay showed that circCBLB bound to the 3' untranslated region (3'UTR) of miR-486-5p. Compared with the model group at the same time point, the cell viability of the overexpression group was lower, while that of the interference group was higher. Compared with the model group, the overexpression group had a higher apoptosis rate, a higher proportion in S and G2 phases, a lower colony formation rate, a lower miR-486-5p expression level, higher IL-4 and IL-10 levels, and lower IL-6 and TNF-α levels. The interference group had a lower apoptosis rate, a lower proportion in S and G2 phases, a higher colony formation rate, a higher miR-486-5p expression level, and a higher TNF-α level. The pcDNA3.1-circCBLB combined with miR-486-5p-mimics group reversed the effects of circCBLB on cell viability, apoptosis rate, cell cycle, colony formation ability, antiinflammatory cytokines, and proinflammatory cytokines. Conclusion circCBLB inhibits the viability of RA-FLS, increases apoptosis rate, prolongs the cell cycle, reduces colony formation ability, increases antiinflammatory cytokines, and decreases proinflammatory cytokines. In contrast, miR-486-5p has opposite regulatory effects on circCBLB and can partially reverse and offset the effects of circCBLB.