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Objective To establish the review rules of Sysmex XN-9000 blood analyzer assembly line for the laboratory to ensure the accuracy of the experimental results. Methods 18 863 blood samples were collected from the hospital from 6 to 31 May 2019, including 4 400 from 6 to 10 and 14 463 from 11 to 31. The samples that violate the review rules were rechecked. Results The sensitivity, specificity, positive predictive value, negative predictive value and total effective rate of 4400 samples were 100.00%, 96.78%, 57.10%, 100.00% and 96.91% respectively. According to the review rules, 617 samples of 4 400 samples were rechecked artificially, including 295 true positive, 22 false positive, 297 true negative and 3 false negative. The positive predictive value was 93.10%, the negative predictive value was 99.00%, and the false negative rate was 1.00%. Every day, 60 cases of negative samples that did not violate the review rules were randomly selected for smear microscopy, 300 samples were randomly examined in 5 days, and 3 false negative samples were found. The sensitivity, specificity, positive predictive value, negative predictive value and total effective rate were 100.00%, 95.81%, 56.24%, 100.00% and 96.02% respectively. Conclusion Under the premise that the false negative rate is less than 5%, especially the leukemia is not missed, the review rules of Sysmex XN-9000 blood analyzer assembly line in the laboratory effectively reduce the retest rate, reduce the workload, and have obvious advantages. [ABSTRACT FROM AUTHOR]

Objective To investigate the effect of lapatinib on cell proliferation and apoptosis in acute myeloid leukemia HL60 cells in vitro and the related molecular mechanisms. Methods The HL60 cells were treated with 5, 10, 15 μmol/L lapatinib for 24 hours, and then morphological changes of the cells were observed under optical microscope. CCK-8 assay was used to assess the cell viability. Colony formation assay was performed to detect the cell proliferation ability. Cell apoptosis labeled by annexinV-FITC/PI were analyzed by flow cytometry. Wright modified LIU's staining and Hoechst33342 fluorescent staining were used to observe the morphology of the nucleus. Western blotting was utilized to detect the expressions of Bax, Bcl2, caspase-3, caspase-9, cleaved caspase-3, cleaved caspase-9, cleaved poly-ADP-ribose polymerase (cleaved PARP), cell proliferation regulating inhibitor of protein phosphatase 2A (CIP2A), c-MYC, AKT and p-AKT. Results Compared with the control group, lapatinib inhibited cell proliferation and promoted apoptosis, induced nuclear fragmentation, chromatin condensation of HL60 cells in a dose-dependent manner. Meanwhile, it down-regulated the expression of Bcl2, up-regulated the levels of Bax, cleaved caspase-3, cleaved caspase-9 and cleaved PARP, and decreased the levels of CIP2A, p-AKT and c-MYC. Conclusion Lapatinib could inhibit cell proliferation and promote apoptosis in HL60 cells by inhibiting the CIP2A/AKT/c-MYC signal pathway.