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Fe3O4 nanoparticles were synthesized by co-precipitation method. 3D graphene was modified on the surface of Fe3O4 nanoparticles, and then covered with tetraethyl orthosilicate(TEOS) and vinyltrimethoxysilane (VTMO). Finally, the magnetic functional material Fe3O4@3DG@VTMO@MPS was synthesized by the polymerization of functional monomer 3-mercapto-1-propanesulfonate(MPS) on the surface using "thiol-ene" click chemistry. The structure, morphology and thermal stability of the functional materials were characterized by scanning electron microscopy(SEM), energy dispersive spectroscopy (EDS), dynamic light scattering (DLS), Fourier transform infrared spectroscopy(FLlR) and thermogravimetric analysis(TGA), respectively. The adsorption properties of this functional material for Lysozyme were studied by static adsorption experiments. The results show that the magnetic functional materials had nice adsorption capacity (the maximum adsorption capacity was 162.1 mg/g) and fast adsorption kinetics(the adsorption equilibrium could be reached in 150 min). The pseudo-second-order kinetic model was suitable for describing the adsorption kinetics of lysozyme on functional materials, and the adsorption process of functional materials on lysozyme was more in line with Langmuir adsorption model, indicating that adsorption is monolayer adsorption. Fe3O4@3DG@VTMO@MPS as solid phase extraction materials as solid phase extraction materials could be used for separation and enrichment of lysozyme, then combined with high performance liquid chromatography (HPLC) to detect honey in actual samples. [ABSTRACT FROM AUTHOR]