[Objective】The paper aimed to study the treatment effect of Sanhuang powder and Biotronic on Streptococcus iniae disease of hy- brid sturgeon. [Method] Hybrid sturgeons infected with S. iniae were treated with 0.5% Sanhuang powder, 0.1% Biotronic and 0.5% Sanhuang powder +0.1% Biotronic. The treatment effect was determined by the mortality, blood biochemical indexes and histopathological changes of the diseased hybrid sturgeons. [Result】 Mortality rate of positive control, Sanhuang powder group, Biotronic group, and Sanhuang powder + Biotronic group was 54.6%, 35.1%, 32%, and 19.6%, respectively; Activities of AKP, ACP, LZM and SOD in serum of dis- eased hybrid sturgeons firstly increased and then decreased, and POD activity decreased initially, followed by increasing, but decreased thereafter. Changes of serum AKP, ACP, LZM, SOD and POD activities of Sanhuang powder group, Biotronic group and Sanhuang powder + Biotronic group were consistent with the positive control group, but the range of changes was all significantly lower than the positive con- trols, with the smallest changes in the Sanhuang powder + Biotronic group; Histopathology of diseased hybrid sturgeons showed that the hepatocytes were seriously degenerated and necrotic, accompanied by a large number of inflammatory cell infiltration, intestinal mucosa and villus were seriously damaged and necrotic, accompanied by inflammatory cell infiltration. Pathological symptoms of liver intestinal of San- huang powder group and Biotronic group reduced compared to positive control group, moreover, liver intestinal pathological symptoms of San- huang powder + Biotronic group were the lightest. [Conclusion] Both Sanhuang powder and Biotronic have a certain therapeutic effect on S. iniae disease of hybrid sturgeon, combination of Sanhuang powder and Biotronic show a synergistic and better treatment effect. [ABSTRACT FROM AUTHOR]