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Objective The aim of this study was to explore the effect of aescine on the growth in cultured rat cerebral cortical neurons and the relationship with BDNF and TrkB. Methods The cerebral cortex cells from newborn SD rats were primarily cultured in 24 or 96 well plate. Cells were randomly divided into normal, control and aescine groups. Aescine group was cultured with 20 mg/mL aescine (diluted with physiological saline) , control group was cultured with the same volume of normal saline, and normal group do not have any treatment. The sub groups and images were captured at 24 h and 48 h. The expression of BDNF and TrkB mRNA were detected by using RT-PCR, and the neuronal growth vigor was detected by MTT. Results There was no difference in normal and control groups at each time point in cells numbers, body area, neurite length and viability (P> 0.05) , but all indicators have increased in the normal and control groups with the time prolonged. The indicators in aescine group were significantly higher than those in normal and control groups (P < 0.05) . RT-PCR showed that there was no significant difference in expression of BDNF and TrkB mRNA in normal and control groups at each time point (P > 0.05), but the expression in aescine group was significantly higher than that in normal and control groups at each time point (P < 0.05). Conclusion Aescine can promote neuron growth and may be relationship with the expression of BDNF and its receptor TrkB. [ABSTRACT FROM AUTHOR]

Objective To evaluate the results of orthodontic micro- implant anchorage implantation by application of Spiral CT and three-dimensional reconstruction techniques, so as to explore its application value in assisting clinical diagnosis and treatment. Methods 20 orthodontic patients were implanted with 48 micro- planting screws. SOMATOM Sensation 40/64 helical CT scan was performed. The axial unenhanced helical CT data were given multi-planar reconstruction (MPR), maximum intensity projection (MIP), volume rendering (VR) and surface multidimensional reconstruction (CPR) by using the Siemens 3D post-processing software. The axial scanning images and reconstructed images were compared, observed and analyzed . Results The planting results of 37 micro screws were satisfactory without injuring the vital tissues surrounding the root. The planting results of 11 micro-screw were not ideal, and these micro-screws were observed or reimplanted and got success. Spiral CT axial scan showed better the tooth root interval and the relationship between tooth root and the planting screws. VR can directly showed the overall relationship between the planting screws and teeth, jaw contour appearance. The relationship between the screw and tooth was showed satisfactorily by MIP which could rotate freely and observe from different angles. MPR could clearly show the structural relationships between micro-root planting screws and tooth root and surrounding tissues from the cross section, and could make up for the shortcomings of axial limitations, and was easy to measure the distance and tilt angle of the long axis. Conclusion Spiral CT and three-dimensional reconstruction can accurately show the jaw micro planting screw shape, location and spatial relationships with adjacent teeth, the image is clear, intuitive and definite, and spiral CT and three-dimensional reconstruction has high application value in orthodontics micro-implant anchorage clinic as a powerful auxiliary examination means. [ABSTRACT FROM AUTHOR]