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To study the relationship between peripheral blood Toll like receptor 4 (TLR4), interleukin-1β (IL-1β) and neutrophil-to-lymphocyte ratio (NLR) and the progression and prognosis of acute pancreatitis (AP). Methods A total of 250 patients with AP were divided into the mild group (121 cases), the moderately severe group (89 cases) and the severe group (40 cases) according to the disease severity. Patients were divided into the poor prognosis group (33 cases) and the good prognosis group (217 cases) based on the prognosis after 5 days of treatment. Peripheral blood levels of TLR4, IL1β and NLR were compared at different time points, different conditions and different prognosis in patients. The correlation of peripheral blood levels of TLR4, IL-1β and NLR, Ranson score and amylase were analyzed by Pearson correlation analysis. The value of each indicator in the diagnosis of severe AP was analyzed using ROC curve, and multivariate Logistic regression analysis was conducted to identify the prognostic risk factors. Results TLR4, IL-1β and NLR levels in AP patients were higher 24 h after admission than 48 h and 72 h after admission (P＜0.05). Peripheral blood TLR4 and IL-1β levels in AP patients at 24 h after admission increased with the condition became worse. NLR level was higher in the severe group than that in the moderately group or the mild group (P＜0.05). Peripheral blood TLR4, IL-1β and NLR levels in AP patients 24 h after admission were positively correlated with Ranson score at 48 h after admission and amylase level at 24 h after admission (P＜0.05). The area under ROC curve, sensitivity and specificity of the combination of TLR4, IL-1β and NLR at 24 h after admission for predicting severe AP were 0.895, 84.53% and 81.69%. Peripheral blood TLR4, IL-1β and NLR levels 24 h after admission were higher in the poor prognosis group than those in the good prognosis group (P＜0.05). Multivariate Logistic regression analysis showed that elevated TLR4, IL-1β and NLR at 24 h after admission were independent risk factors for poor prognosis of patients with AP (P＜0.05). Conclusion Peripheral blood TLR4, IL-1β and NLR in patients with AP are closely related to disease progression and prognosis. Therefore, they can be taken as important monitoring indicators. [ABSTRACT FROM AUTHOR]

Objective To analyze the expression levels of serum transcription factor PU.1 and interleukin-4 (IL-4) in children with acute upper respiratory tract infectious diseases and explore their clinical diagnostic values. Methods A total of 78 children with acute upper respiratory tract infection treated in our hospital were selected as the disease group, and 78 healthy children with physical examination during the same period were selected as the normal control group. Fluorescence quantitative method and enzyme linked immunosorbent assay (ELISA) were used to detect the expression levels of serum transcription factors PU.1 and IL-4 in children respectively. The receiver operating characteristic (ROC) curve was used to analyze its clinical diagnostic value. Multivariate Logistic regression was used to analyze the independent risk factors affecting the occurrence of acute upper respiratory tract infectious diseases. Results The serum levels of interleukin-6 (IL6), C reactive protein (CRP), procalcitonin (PCT), tumor necrosis factor alpha (TNF-α), IL-4 and the relative expression of PU.1 were significantly higher in the disease group than those in the normal control group (P＜0.05). The areas under the ROC curve of serum IL-4 level and the relative expression of PU.1 and their combined detection for diagnosing the upper respiratory tract infectious diseases in children were 0.752, 0.855 and 0.984, respectively, the corresponding sensitivities were 58.97%, 78.21%, 94.87%, respectively, and the specificities were 83.33%, 78.21% and 97.44%, respectively. A multivariate Logistic regression analysis was performed, and the results showed that higher serum IL-4, PU.1, IL-6, CRP and TNF-α expression levels were independent risk factors affecting the occurrence of upper respiratory tract infectious diseases (P＜0.05). Conclusion The serum levels of PU. 1 and IL-4 in children with acute upper respiratory tract infectious diseases are relatively high, which have certain diagnostic value. [ABSTRACT FROM AUTHOR]

Objective To investigate the protective effects of active components of notoginseng on immunological liver injury in mice. Methods Sixty Kunming mice were randomly divided into normal group (group A), model group (Group B), PNS group (Group C), total flavonoids group (Group D),and polysaccharide group (group E). C,D and E groups were given PNS, total flavonoids and polysaccharide orally, 1/day for 14 days. Then BCG was intravenous injected in B, C, D and E groups, and after 26 days lipopolysaccharide (LPS) was intravenous injected. Samples of eye venous plexus blood were collected, and serum levels of alanine aminotransferase (ALT), total superoxide dismutase (T-SOD) and Interleukin-4 (IL-4) were detected. Organs index was calculated by checking pathological results of liver, spleen and thymus. Results Compared with group B, the thymus index and serum ALT levels were significantly decreased in C, D and E groups (P < 0.01), but the levels of IL-4 and T-SOD increased significantly (P< 0.01). Pathological results showed that there were more serious inflammatory cell infiltration in liver, edema and necrosis of dot in C, D and E groups than those in group B. Conclusion The active components of notoginseng showed a significant protective effect on immunological liver injury. [ABSTRACT FROM AUTHOR]