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To study the effect of Xiaoer Xiaoji Zhike Oral Liquid combined with budesonide on serum interferon-γ (IFN-γ), interleukin-4 (IL-4), transforming growth factor β1 (TGF-β1), nitric oxide (NO) level in children with bronchial asthma. Selected 67 cases of patients with bronchial asthma who were treated in our hospital from January 2018 to December 2019, divided into two groups randomly. The control group was treated with budesonide alone, and the observation group was treated with Xiaoer Xiaoji Zhike Oral Liquid combined with budesonide. The effective rate of the observation group 93.94 % was significantly higher than that of the control group 70.59 % (P<0.05). The disappearance time of wheezing, cough disappearance, hospital stay and wheezing disappearance time of the observation group were significantly shorter than control group (P<0.05). After treatment, the levels of serum NO and IL-4 of the two groups of children were significantly reduced (P<0.05), and the levels of serum TGF-β1 and IFN-γ were significantly increased (P<0.05), and the levels of serum NO and IL-4 in the observation group The level of IL-4 was significantly lower than that of the control group (P<0.05), and the levels of serum TGF-β1 and IFN-γ were significantly higher than that of the control group (P<0.05). After treatment, the serum IgM, IgA and IgG levels of the two groups of children were significantly reduced (P<0.05), and the serum IgM, IgA and IgG levels of the observation group were significantly lower (P<0.05). Erxiaoji Zhike Oral Liquid combined with budesonide has a significant effect on children with bronchial asthma. The mechanism may be related to the regulation of serum IFN-γ, IL-4, TGF-β1 and NO levels. [ABSTRACT FROM AUTHOR]

Objective: To explore the changes of plasma leptin, interleukin-4 (IL-4), interleukin-18 (IL-18) and tumor necrosis factor - α (TNF-α) levels in patients with nephrotic syndrome and their clinical significance. Methods:110 cases of nephrotic syndrome diagnosed and treated in our hospital from February 2018 to October 2019 were selected as the observation group, and 110 healthy patients who underwent physical examination in our hospital during the same period were selected as the control group. The plasma leptin, IL-4, IL-18, TNF-α, 24 h proteinuria and plasma albumin levels were measured and compared between the two groups. The correlation of plasma leptin, IL-4, IL-18 and TNF-α with 24 h proteinuria and plasma albumin were analyzed by Pearson method. The diagnostic value of each index to nephrotic syndrome was analyzed by receiver operating characteristic curve (ROC). Results: Compared with the control group, the plasma leptin, IL-4, IL-18 and TNF-α levels in the observation group were significantly increased (P<0.05). Compared with the control group, 24 h proteinuria in the observation group was significantly increased, and plasma albumin level was significantly decreased (P<0.05). The plasma leptin, IL-4, IL-18 and TNF-α levels were positively correlated with 24 h proteinuria, while negatively correlated with plasma albumin (P<0.05). The diagnostic value of plasma leptin, IL-4, IL-18 and TNF-α in the combined diagnosis of nephrotic syndrome was significantly higher than that of single diagnosis of plasma leptin, IL-4, IL-18 and TNF-α. Conclusions: The plasma leptin and inflammatory cytokines levels are significantly increased in patients with nephrotic syndrome, which are correlated with the patients' 24 h proteinuria and plasma albumin. The combined detection of plasma leptin, IL-4, IL-18 and TNF-α is of high diagnostic value for nephrotic syndrome. [ABSTRACT FROM AUTHOR]

Objective: To prepare recombinant adeno-associated virus harboring truncated murine interleukin-4 mutant gene and detect the exogenous protein expression in vitro. Methods: An m IL-4 antagonistic mutant DNA expression plasmid pSNAV-mIL-4ΔC22 was constructed through gene clone technology, a recombinant virus vector r AAV5-mIL-4ΔC22 was prepared by three plasmids co-transfection, human bronchoid epithelioid cell line 16 HBE and BEAS-2 B were transfected with recombinant AAV5 vectors, then the expression of foreign protein was detected by Western blot and ELISA. Results: The mutant gene m IL-4ΔC22 was verified by DNA sequencing and the titer of virus vector r AAV5-mIL-4ΔC22 is about 3×1011vg/m L. 16 HBE and BEAS-2 B expressed GFP lastly and stably when 36 hours after receiving r AAV-GFP trensfection. Conclusions: Our study constructed recombinant vector r AAV5-mIL-4ΔC22 encoding murine IL-4 antagonistic mutant protein deleting the C-terminus region, the virus vector effectively transfected 16 HBE and BEAS-2 B, and mediated exogenous protein secretory expression in vitro. [ABSTRACT FROM AUTHOR]

Objective:To analyze the clinical characteristics of refractory mycoplasmal pneumonia (RMPP) in children with refractory pneumonia and the predictive value of serum interleukin-4(IL-4), interleukin-6(IL-6), interleukin-10(IL-10) and interferon-γ (IFN-γ ) detection for RMPP. Methods: A retrospective analysis of 453 cases of children with mycoplasma pneumoniae pneumonia (MMP) clinical data whocollected from June 2014 to January 2014 in our hospital, including 81 cases of RMPP and 372 cases of general MPP (GMPP), the clinical features, clinical manifestations, chest imaging, extrapulmonary complications, laboratory examination results in the both groups were analyzed and compared, multivariate Logistic regression was used to analyze the related factors of RMPP, the ROC curve was used to analyze the predictive value of each factor on RMPP. Results: Age, warmth, fever, chills, breath sounds, lung dullness, pleural effusion, lobar pneumonia, heart damage, oxygenation rate, hospital stay, levels of white cell count, neutrophil percent- age, prealbumin, serumof IgM , IgA, IgG, IL-4, IL-6, IL-10, IFN-γ in the RMPP group were significantly higher than GMPP group, there was a statistically significant difference(P<0.05). Multivariate logistic regression analysis showed that serum of IL-4, IL-6, IL-10, and IFN-γ levels were independent risk factors for RMPP(P<0.05). Areas under of serumof IL-4, IL-6, IL-10, IFN-γ , and the combined detection of the four groups were 0.681, 0.699, 0.693, 0.767, and 0.857, the area under the combined detection curve was significantly higher than that of serum of IL-4, IL-6, IL-10, IFN-γ were detected separately. Conclusion: RMPP children is relatively elder, the clini- cal manifestations, chest imaging, and extrapulmonary complications were more severe in children than those with GMPP, they need more oxygen therapy, extended hospital stay, the serumlevels of IL-4, IL-6, IL-10 and IFN-γ of RMPP children were significantly in- creased and the combined detection of these four cytokines is of high clinical value for predicting the occurrence of RMMP. [ABSTRACT FROM AUTHOR]

Objective: To study clinical research of ulinastatin in treatment of bronchial asthma and analysis of the changes of serum interleukin (IL)-2, IL-4 and T cell subsets. Methods: 98 patients of bronchial asthma who received therapy from January 2014 to October 2016 in our hospital were selected. According to random number table, those patients were divided into the observation group (n=49) and the control group (n=49). The control group was treated with routine treatment, including anti inflammation, improve ventilation, correct acid-base balance disorders, inhaled corticosteroids and β2 receptor agonists, electrolytes, etc, while the observation group was combined with ulinastatin treatment. The clinical efficacy and clinical symptom regression time were compared, and collection 3ml fasting venous blood was collected before and after treatment, the changes of serum IL-2, IL-4 and T cell subsets were detected by flow cytometry. Results: After treatment, the total effective rate in the observation group was significantly higher than that of the control group (P＜0.05); the dyspnea, wheeze, chest tightness, cough symptoms subsided time in the observation group was significantly shorter than that of the control group (P ＜0.05); before treatment, there was no significant difference in serum IL-2 and IL-4 between the two groups(P＞0.05), compared with before treatment, the serum IL-2 and IL-4 were significantly improved in the two groups after treatment (P ＜0.05), the serum IL-2 in the observation group was significantly higher than that of the control group, the serum IL-4 was significantly lower than that of the control group (P＜0.05); There was no significant difference in CD3+, CD4+, CD8+ and CD4+/CD8+ between the two groups (P＞0.05), compared with before treatment, the CD3+, CD4+, CD8+ and CD4+/CD8+were significantly improved in the two groups after treatment (P＜0.05), the CD3+, CD4+ and CD4+/CD8+ in the observation group were significantly higher than those of the control group, and the CD8+was significantly lower than that of the control group (P＜0.05). Conclusion: Ulinastatin is well for bronchial asthma, which can effectively relieve clinical symptoms, to improve the expression of serum IL-2, IL-4 and T cell subsets, regulation of immune function, high clinical value. [ABSTRACT FROM AUTHOR]