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Objective: To investigate the effects of acupoint application combined with staged rehabilitation function training on functional rehabilitation, serum pain factors and bone metabolism markers in patients with femoral neck fracture after operation. Methods:150 patients with femoral neck fracture who were treated in Sichuan Orthopedic Hospital from January 2021 to October 2021 were included. According to the envelope lottery method, the patients were divided into control group (75 cases) and observation group (75cases) The control group received staged rehabilitation function training, and the observation group received acupoint application combined with staged rehabilitation function training. Both groups were intervened for 3 months. After 3 months, the functional rehabilitation, visual pain analogue scale (VAS) score, serum pain factors and bone metabolism markers in the two groups were observed, and the incidence of postoperative complications in the two groups was recorded. Results: 3 months after intervention, Barthel Index and Harris hip score in the two groups were higher than those before treatment, and the observation group was higher than that in the control group (P＜0.05) 1 month after intervention, 2 months after intervention and 3 months after intervention, the scores of visual pain analogue scale (VAS) in the two groups decreased gradually, and the scores in the observation group were lower than those in the control group (P＜0.05) 3months after intervention, bone specific alkaline phosphatase (BALP) and osteoprotegerin (OPG) increased in the two groups, and the observation group was higher than the control group. The levels of tartrate resistant hydrochloric acid phosphatase isomer (TRACP-5b), type I collagen carboxyl terminal peptide β Special sequence (β-CTX) were decreased, and the observation group was lower than the control group (P＜0.05) 3 months after intervention, the levels of bradykinin (BK), prostaglandin E2 (PGE2), 5-hydroxytryptamine (5-HT) and neuropeptide Y (NPY) in two groups were decreased, and the observation group was lower than the control group (P＜0.05). There was no significant difference in the incidence of postoperative complications between the two groups (P＞0.05) Conclusion: Acupoint application combined with staged rehabilitation function training can promote the postoperative functional recovery of patients with femoral neck fracture and reduce postoperative pain, which may be related to regulating the level of markers related to bone metabolism. [ABSTRACT FROM AUTHOR]