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To study the effect of Xiaoer Xiaoji Zhike Oral Liquid combined with budesonide on serum interferon-γ (IFN-γ), interleukin-4 (IL-4), transforming growth factor β1 (TGF-β1), nitric oxide (NO) level in children with bronchial asthma. Selected 67 cases of patients with bronchial asthma who were treated in our hospital from January 2018 to December 2019, divided into two groups randomly. The control group was treated with budesonide alone, and the observation group was treated with Xiaoer Xiaoji Zhike Oral Liquid combined with budesonide. The effective rate of the observation group 93.94 % was significantly higher than that of the control group 70.59 % (P<0.05). The disappearance time of wheezing, cough disappearance, hospital stay and wheezing disappearance time of the observation group were significantly shorter than control group (P<0.05). After treatment, the levels of serum NO and IL-4 of the two groups of children were significantly reduced (P<0.05), and the levels of serum TGF-β1 and IFN-γ were significantly increased (P<0.05), and the levels of serum NO and IL-4 in the observation group The level of IL-4 was significantly lower than that of the control group (P<0.05), and the levels of serum TGF-β1 and IFN-γ were significantly higher than that of the control group (P<0.05). After treatment, the serum IgM, IgA and IgG levels of the two groups of children were significantly reduced (P<0.05), and the serum IgM, IgA and IgG levels of the observation group were significantly lower (P<0.05). Erxiaoji Zhike Oral Liquid combined with budesonide has a significant effect on children with bronchial asthma. The mechanism may be related to the regulation of serum IFN-γ, IL-4, TGF-β1 and NO levels. [ABSTRACT FROM AUTHOR]

To study effect of symbicort turbuhaler (ST) combined with montelukast on pulmonary function and serum cytokine levels in patients with acute stage of bronchial asthma(ASBA). 86 patients with ASBA who were treated in our hospital from February 2015 to August 2017 were selected as research subjects. The patients were divided into the observation group (n=43) and the control group (n=43) according to the random number table method. The control group was treated with ST, and the observation group was treated with montelukast on the basis of the control group, two groups were treated for 3 months. The lung function, cytokine levels, cough score, St. George's respiratory disease questionnaire (SGRQ) before treatment and 3 months after treatment and drug safety were compared between the two groups. After treatment, the levels of forced expiratory volume (FEV1), forced vital capacity (FVC) and FEV1/FVC in the two groups were increased significantly compared with those before treatment, and the observation group was higher than that of the control group (P<0.05). After treatment, the levels of interleukin-4 (IL-4), interleukin-5 (IL-5), interferon-酌 (INF-酌) and tumor necrosis factor-琢 (TNF-琢) in the two groups were all lower than those before treatment, and the observation group was lower than that in the control group (P<0.05). After treatment, the scores of cough in two groups were significantly higher than before treatment, and the observation group was significantly higher than the control group(P<0.05). After treatment, the scores of SGRQ in two groups were significantly lower than before treatment, and the observation group was significantly lower than the control group(P<0.05). The total incidence of adverse reactions in the observation group was 20.93%, and there was no statistical difference compared with 16.28% of the control group (P>0.05). ST combined with montelukast can effectively improve the symptoms of the ASBA patients, alleviate the inflammatory reaction of the body and improve the pulmonary function and quality of life of the patients at the same time. [ABSTRACT FROM AUTHOR]

Objective: To study clinical research of ulinastatin in treatment of bronchial asthma and analysis of the changes of serum interleukin (IL)-2, IL-4 and T cell subsets. Methods: 98 patients of bronchial asthma who received therapy from January 2014 to October 2016 in our hospital were selected. According to random number table, those patients were divided into the observation group (n=49) and the control group (n=49). The control group was treated with routine treatment, including anti inflammation, improve ventilation, correct acid-base balance disorders, inhaled corticosteroids and β2 receptor agonists, electrolytes, etc, while the observation group was combined with ulinastatin treatment. The clinical efficacy and clinical symptom regression time were compared, and collection 3ml fasting venous blood was collected before and after treatment, the changes of serum IL-2, IL-4 and T cell subsets were detected by flow cytometry. Results: After treatment, the total effective rate in the observation group was significantly higher than that of the control group (P＜0.05); the dyspnea, wheeze, chest tightness, cough symptoms subsided time in the observation group was significantly shorter than that of the control group (P ＜0.05); before treatment, there was no significant difference in serum IL-2 and IL-4 between the two groups(P＞0.05), compared with before treatment, the serum IL-2 and IL-4 were significantly improved in the two groups after treatment (P ＜0.05), the serum IL-2 in the observation group was significantly higher than that of the control group, the serum IL-4 was significantly lower than that of the control group (P＜0.05); There was no significant difference in CD3+, CD4+, CD8+ and CD4+/CD8+ between the two groups (P＞0.05), compared with before treatment, the CD3+, CD4+, CD8+ and CD4+/CD8+were significantly improved in the two groups after treatment (P＜0.05), the CD3+, CD4+ and CD4+/CD8+ in the observation group were significantly higher than those of the control group, and the CD8+was significantly lower than that of the control group (P＜0.05). Conclusion: Ulinastatin is well for bronchial asthma, which can effectively relieve clinical symptoms, to improve the expression of serum IL-2, IL-4 and T cell subsets, regulation of immune function, high clinical value. [ABSTRACT FROM AUTHOR]

Objective To investigate the clinical significance of serum eosinophil cationic protein(ECP), Interleukin-2 (IL-2), IL-4, IL-5 and gamma interferon(IFN-γ)in children with acute attack of bronchial asthma. Methods A total of 40 cases of children with bronchial asthma treated in the pediatric asthma outpatient and ward of the hospital in Xiamen Hospital of Beijing University of Chinese Medicine from January 2015 to June 2017 were selected as the observation group,30 healthy children of the same age were selected as the control group. Levels of above five serum inflammatory cytokines were detected before treatment and 2 weeks after treatment in the two groups.the correlation between serum inflammatory cytokines and asthma severity in children with asthma were analyzed. Results (l)The levels of serum ECP, IL-2, IL-4, IL-5, IFN-γ before treatment in the observation group were compared with those in the control group, the differences were statistically significant P<0.05); (2)The levels of serum IL-2.IFN-γ in asthmatic children were negatively correlated with the severity of asthma (rs =— 0.940, — 0.938, P<0.05), but ECP,lL-4 and IL-5 were positively correlated with the severity of asthma(rs = 0.944, 0.920, 0.915, P<0.05);(3)After two weeks treatment, the levels of ECP, IL-4 and IL-5 in the observation group were decreased, while the levels of IL-2 and INF-γ were increased, the differences were statistically significant P<0.05). Conclusion The combined detection of ECP, IL-4, IL-5, IL-2,IFN-γ is helpful for the diagnosis of bronchial asthma, and judging the change of the disease. [ABSTRACT FROM AUTHOR]

Objective To observe the effect of aerosol inhalation of L-arginine on acute airway inflammation in mice model with asthma and to investigate the mechanism. Methods Thirty male BALB / c mice were randomly divided into three groups and 10 mice in each group: L-arginine group,model group and the control group. The L-arginine group and model group were sensitized by intraperitoneal( i. p.) injections of 0. 3 m L suspension of 100 μg OVA and 2 mg AL( OH)3,and the control group was injected with 0. 3 m L PBS at the same time. Since the 21 st day,these three groups were put into homemade glass atomization box. L-arginine group and model group were given 30 g / L OVA for aerosol inhalation and the control group with PBS for aerosol inhalation,once a day,thirty minutes one time and for 12 days. L-arginine group was given 174 mg / m L L-arginine for aerosol inhalation for 30 minutes before every sensitization. Twenty-four hours after the last sensitization,mice of the three groups were bled to death,and then we collected the left lung tissues and the bronchoalveolar lavage fluid. HE staining was used on the upper left lobe of lung tissue,and the pathological changes were observed. We counted the total number of leukocytes,esosinophils,lymphocytes and macrophages. The levels of interlerkin-4( IL-4),NO and interferon-γ( IFN-γ) in BALF were measured by ELISA. Results In the model group,the airway epithelium fractured, fell off and had mucous edema with more folds under HE staining, and there was inflammatory cell infiltration with a large number of esosinophils and lymphocytes around the bronchus. The inflammatory cell infiltration and mucous edema in the L-arginine group was less than that of the model group. Compared with the control group,the leukocytes, esosinophils, lymphocytes and macrophages in BALF were increased in the L-arginine group and the model group, and the above indexes of the L-arginine group were less than those of the model group( all P 0. 05). Compared with the control group, the levels of IL-4 and IL-4 / IFN-γ were increased, but the levels of IFN-γ and NO were decreased in the Larginine group and model group; compared with the model group, the levels of IL-4 and IL-4 / IFN-γ declined, but IFN-γand NO elevated in the L-arginine group, and significant difference was found among these groups( all P 0. 05). Conclusion The aerosol inhalation of L-arginine can inhibit the airway inflammation of asthmatic mice, and its mechanism may be related to inhibiting IL-4 expression, promoting IFN-γ expression and the production of NO. [ABSTRACT FROM AUTHOR]