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Copyright of Journal of Beijing University of Traditional Chinese Medicine is the property of Journal of Beijing University of Traditional Chinese Medicine and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Uric acid-lowering therapy for hyperuricemia complicated with chronic kidney disease can delay progress of chronic kidney disease. Western and traditional Chinese medicines have clear effects for reduction of uric acid. However, for chronic kidney disease complicated with hyperuricemia, the application of western medicine has its limitation. This paper reviewed recent studies on uric acid-lowering effects of western and traditional Chinese medicine in the treatment of hyperuricemia complicated with chronic kidney disease, in order to provide reference for the combined medication of western and traditional Chinese medicine in treatment of hyperuricemia with chronic kidney disease. [ABSTRACT FROM AUTHOR]

Copyright of Pharmaceutial Care & Research is the property of Editorial Board of Pharmaceutial Care & Research and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Objective To investigate the mechanism of iron death induced by TRPC6/NF-κB in glomerular podiocytes mediated by high homocysteine (Hey). Methods Mouse glomerulopocytes were cultured in vitro and divided into Control group (0 μmol/L Hey) and Hcy group (80 μmol/L Hey). After 48h of intervention, Western blot was used to detect the expression levels of iron death related proteins GPX4 and SLC7A11 and TRPC6 and NF-κB. Real-time quantitative fluorescence PCR (qRT-PCR) and immunofluorescence were used to detect the expression of TRPC6. The level of podocyte apoptosis was detected by flow cytometry. Malondialdehyde (MDA) assay kit was used to determine intracellular MDA levels. After transfection of TRPC6 interference fragment and TRPC6 negative control (NC), qRT-PCR was divided into Control, si-NC and si-TRPC6 (Si-TRPC6-1, Si-TRPC6-2, Si-TRPC6-3). Western Blot was divided into Control, Hey, si-NC+Hcy, si-TRPC6+Hcy.The expression of TRPC6 mRNA was detected by qRT-PCR. The expression levels of GPX4, SLC7A11, NF-κB and TRPC6 were detected by Western Blot. The level of podocyte apoptosis after interference was detected by flow cytometry. Results (l)Compared with Control group, the expression levels of iron death related proteins GPX4 and SLC7A11 in Hcy group were decreased, and the apoptosis rate was increased (P < 0.05). (2) Compared with Control group, TRPC6 protein, mRNA levels and immunofluorescence expression were increased in Hcy group.The level of MDA and the expression of NF-κB signaling pathway protein increased in Hcy group, and the comparison between the two groups had statistical significance (P < 0.05). (3) Compared with the si-NC group, the mRNA expression level of TRPC6 in si-TRPC6 (Si-TRPC6-1, Si-TRPC6-2, Si-TRPC6-3) group was decreased, and the interference effect of Si-TRPC6-3 was the best(P < 0.05). After transfecting TRPC6 NC and TRPC6 interference fragment and administering Hey, there was no difference in GPX4, SLC7A11, NF-κB and TRPC6 expression in si-NC+Hcy group compared with Hcy group. Compared with the si-NC+Hcy group, the si-TRPC6+Hcy group had higher expression of iron death related proteins, GPX4 and SLC7A11, lower expression of NF- κB and TRPC6, and decreased apoptosis rate (P < 0.05 ). Conclusion This study confirmed that TRPC6/NF-κB can regulate iron death of renal podocytes under the induction of Hcy, which is one of the mechanisms leading to kidney injury. [ABSTRACT FROM AUTHOR]

Copyright of Progress in Modern Biomedicine is the property of Publishing House of Progress in Modern Biomedicine and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)