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Callicarpa integerrima has very good effect in emoving blood stasis and resolving static blood and treatment of rheumatism evil. However, there are few reports on its chemical constituents. In order to explore the chemical constituents from the roots and stems of C. integerrima, the 95% ethanol extracts of C. integerrima roots and stems were isolated and purified by diverse column chromatography, such as silica gel, Sephadex LH-20 gel column, ODS column chromatography, and preparative HPLC. In addition, these compounds were identified on the basis of NMR, ESI-MS as well as other modern spectral techniques. The results were as follows: A total of 15 compounds were isolated from the 95% ethanol extracts of the roots and stems of C. integerrima, which were identified as stigmast-4-en-3-one(1),(24R)-5α-stigmastane-3,6-dione(2), 2'-hydroxy-4'-methoxydihydrochalcone(3), α-amyrin(4), β-sitosterol(5), ursolic acid(6), 4-hydroxy-3-methoxy-benzoic acid(7), 4-pyridinol(8), p-hydroxybenzoic acid(9), forsythoside B(10), nepetifosides D(11), isoacteoside(12), acteoside(13), pedicularioside M(14), β-methoxy forsythoside B(15). All compounds, except for compounds 4-6, 12 and 13, were isolated from C. integerrima for the first time. Furthermore, compounds 1, 2, 3, 8, 11 and 14 were isolated from the genus of Callicarpa for the first time. Thus, the results of this research has enriched the compound library and afford a scientific foundation for the further rational use of C. integerrima. [ABSTRACT FROM AUTHOR]