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Allergic asthma is a chronic disease with an increasing incidence year by year. The typical manifestations are airway inflammation and airway hyperresponsiveness. Airway epithelial cell（ AEC）, as the first barrier against a variety of allergens, can be the first to initiate the innate immune response to participate in allergic asthma. AEC regulates the adaptive immune response by secreting antimicrobial substances, expressing pattern recognition receptors, secreting innate immune factors, participating in innate immune memory and affects the process of allergic asthma. Therefore, AEC is considered to be the regulatory hub of lung inflammation and immune response in allergic asthma. In addition, targeted therapy drugs for the innate immune function of AEC have also been developed rapidly, providing more options for clinical treatment of allergic asthma. [ABSTRACT FROM AUTHOR]

Machine learning was used for idenfifying possible pathogenesis-related long non-coding RNAs (lncRNAs) in labial gland tissue and whole blood of Sjogrens syndrome (SS) patients, and investigating the correlation between the lncRNA with immune cell infiltration in labial gland tissue. The expression data of SS labial gland tissue and whole blood were obtained from the GEO database, and the data were normalized and processed for differential analysis to obtain lncRNA expression profiles. Common lncRNAs were screened and intersected by two machine learning methods. Based on CIBER-SORT software, 22 immune cell infiltration situations in labial gland tissues were calculated,and the correlation between key lncRNAs and immune cell infiltration was analyzed. The correlation between key lncRNAs and clinical traits in whole blood was analyzed and ROC curves were plotted. Key lncRNA co-expression encoding protein genes were analyzed and KEGG signaling pathway analysis was performed. One SS key lncRNA (HCP5) was identified, and HCP5 was upregulated in labial gland tissue and whole blood. Immuno-infiltration analysis showed that the proportion of γδ T cells, macrophages, and CD4+ memory T cells increased in SS labial gland tissue, while the proportion of plasma cells decreased in SS labial gland tissue. The expression of HCP5 was positively correlated with the infiltration of γ/δ T cells and CD4+ memory T cells. In whole blood, HCP5 significantly correlated with ANA, IgG, anti-SSA antibodies, anti-SSB antibodies, and ESSDAI. The ROC diagnostic curve results in different datasets showed that the AUCs of HCP5 were 0.833 and 0.877. KEGG analysis showed that the function of proteins co-expressed with HCP5 in the labial gland was focused on signaling pathways such as antigen processing and presentation and B cell receptor signaling pathway, while the proteins co-expressed with HCP5 in whole blood functioned in signaling pathways such as metabolic pathways and apoptosis. HCP5 may affect the pathogenesis and progression of the disease by regulating the infiltration of immune cells in the labial gland tissue, and may be a potential diagnostic and therapeutic target for SS. [ABSTRACT FROM AUTHOR]

Objective: To investigate the effect of Jianpi Xiaoying Decoction combined with Youjiale on thyroid hormones, thyroid autoantibodies and CD4+CD45RO+ memory T cells in patients with Hashimoto's thyroiditis (HT) due to spleen deficiency. Methods: A total of 60 patients with HT who were treated in our hospital from May 2020 to November 2021 were selected and randomly divided into the control group (treatment with Youjiale, 30 cases) and the study group (Jianpi Xiaoying Decoction combined with After 12 weeks of treatment, the clinical efficacy was observed in both groups, and the changes of TCM syndrome scores, thyroid hormones, thyroid autoantibodies and CD4+CD45RO+ memory T cells were compared between the two groups before and after treatment. Results: The clinical total effective rate of the study group was higher than that of the control group (P<0.05). Thyroid-stimulating hormone (TSH) level, total score of TCM syndrome, percentage of CD4+CD45RO+ memory T cells in peripheral blood mononuclear cells (PBMC), thyroglobulin antibody (TGAb), thyroid peroxide in the study group after treatment The enzyme antibody (TPOAb) was lower than the control group (P<0.05), and the levels of free triiodothyronine (FT3) and free thyroxine (FT4) were higher than those in the control group (P<0.05). Conclusion: The treatment of HT patients with spleen spleen deficiency type combined with Jianpi Xiaoying Decoction can improve their thyroid hormone levels, regulate the level of thyroid autoantibodies and the percentage of CD4+CD45RO+ memory T cells in PBMC, and further improve the clinical efficacy. [ABSTRACT FROM AUTHOR]

To improve the proliferation and activity of adoptive T cell by screening serum-free media for in vitro expansion of flow sorting T cells. Methods: Peripheral blood mononuclear cells from six healthy volunteers were prepared by gradient centrifugation using peripheral blood lymphocyte separation tube. The CD3+T cells from the peripheral blood mononuclear cells were then sorted by flow cytometry into four commonly Lymphocyte culture medium, including X-VIVO 15, KBM 581, TexMACS GMP serum-free media and RPMI 1640 with ten percent of fetal bovine serum complete medium. The status of the cultured cells and the proliferation ability of the cells in vitro was observed and recorded. On the 3rd, 6th and 8th days, viable cell counts were tested by trypan blue staining. On the 8th day, apoptosis was detected by the Annexin V: FITC Apoptosis Detection Kit. The immune phenotype of the expanded cells invitro was detected by the flow cytometer. Results: A small amount of cell debris was observed when X-VIVO 15, TexMACS GMP and RPMI 1640 with ten percent of fetal bovine serum medium were used as the receiving medium for flow cytometry, while a large number of dead cells were observed in samples sorted in KBM 581 (P<0.05). The number of cells expanded in X-VIVO 15 medium is more than that of the other three media, and proliferation testing shows that live cells proliferate rapidly in X-VIVO 15 medium. Rate of apoptosis is significantly lower than that of KBM 581 and TexMACS GMP (P<0.05). The expanded cells mainly show effect memory type in the four different cultural environments. The rate of effect memory T cells in X-VIVO 15 medium was higher than that in TexMACS GMP(P<0.05). The rate of terminal effector T cells in TexMACS GMP medium was higher than that in 10 % FBS/1640. Conclusion: Flow-sorted T cells show higher proliferation capacity, viability and memory phenotype in X-VIVO 15 media, suggesting that X-VIVO 15 media is suitable for in vitro expansion of cells after flow-sorting. [ABSTRACT FROM AUTHOR]