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This experiment aimed at the nutritional value of spirulina powder and its effects on growth performance, apparent metabolic rate and meat quality of broilers. A total of 960 healthy 1-day-old AA broilers (40 g) were selected and randomly divided into 4 treatments by using a single-factor experiment design (control group, test group Ⅰ, test group Ⅱ and test group Ⅲ), with 6 repetitions for each treatment and 40 broilers for each repetition (half male and half female). The 4 treatments were fed with 0, 5, 10, 15 kg/t spirulina powder in the basal diet respectively, and the metabolizable energy and crude protein levels of each treatment group were same. The test period is 42 days, of which the pre-feeding test period is 1-14 days, and the formal test period is 15-42 days. The test results showed that the average daily gain and average daily feed intake of each test group were significantly higher than those of the control group (P<0.05), and there was no significant difference in the feed-to-meat ratio (P>0.05). The apparent metabolic rate of protein in each test group was higher than that in the control group (P<0.05), the apparent metabolic rate of crude fat in the test group Ⅱ and the test group Ⅲ was significantly higher than that in the control group (P<0.05). The apparent metabolic rate of calcium in the test group Ⅰ and Ⅱ was significantly higher than that in the control group (P<0.05). Compared with the control group, the test group showed improvements in slaughter performance and meat quality of broilers. Adding spirulina powder to the diet can significantly increase the growth performance and slaughter performance of broilers, and the optimum adding ratio is 15 kg/t. [ABSTRACT FROM AUTHOR]

In order to produce analytical-grade C-Phycocyanin (C-PC) from Spirulina platensis at low cost and high efficiency, the ultrasonic coupling high-pressure homogenization method was used to improve the yield of C-PC from Spirulina powder. The analytical grade C-PC could be obtained by purification of salting out, dialysis and column chromatography (glucan gel G- 75). Meanwhile, the SDS-PAGE, the color and the UV absorbance were used to characterize the analytical grade C-PC. The results showed that the ultrasonic coupling high-pressure homogenization method could effectively improve the yield of C-PC, and the optimal extraction conditions were as follows: ultrasonic power 390 W for 2 min, 2 s/2 s off; 60 MPa treatment for 3 times. The crude yield and purity (A620/A280) of C-PC were 131.2 mg g-1 and 0.69 under the optimal condition, which were 51.07% and 58.15% higher than those of single high-pressure homogenization and single ultrasound, respectively. The purity of C-PC increased to 1.09 (>0.7) after two steps of salting out (12% and 50% ammonium sulfate) and dialysis, and then could reach 4. 23 (>4.0) after further purification of 25 min chromatography (dextran gel G-75). Finally, the yield of the analytical-grade C- PC was 107.65 mg g-1 with 82.05% recovery rate from crude C-PC extraction. In addition, the L*a*b* values of the analytical-grade C-PC were 58.07,-15.44 and -17.86, respectively, which closely related to purity, concentration, and material. Analysis of SDS-PAGE showed that the analytical-grade C-PC had clear strip and molecular weight of 17 kDa, which was consistent with the previously reported molecular weight (15~21 kDa) of phycocyanin. The results could provide technical support for the production of analytical C-PC with high cost-efficiency. [ABSTRACT FROM AUTHOR]

The experiment was to study the effect of different dietary enhances on growth, body color and intestinal and hepatopancreas biochemical indexes of red-fin Maylandia zebra juvenile. The red-fin Maylandia zebra juvenile with a body weight of 0.12 g were selected as the feeding group (group A), the unfortified brine group (group B), the spirulina powder-enhanced brine group (group C), and the yeast-enhanced brine group (Group D), cod liver oil-enhanced brine group (group E), and algal meal cod liver oil-enhanced brine group (group F), of which group A was the control group, and each group was placed in each tank with 50 experimental fish, with three replicates in each group. The experimental period was 4 w. The results showed that the final body length, body weight, body length and body weight growth rate of experimental group were significantly higher than those of control group (P<0.05), and the data of group D was the highest, with obvious advantages. The fatness of juvenile fish in group D was significantly higher than that in other groups except control group (P<0.05). The L* value of juvenile fish in group C, group D and group F was significantly lower than that in control group (P<0.05). The a* value of juvenile fish in group B, group C and group F was significantly higher than that in control group (P<0.05). The b* value of juvenile fish in group B, group C, group D and group F was significantly higher than that in control group (P<0.05). The intestinal pepsin and trypsin activities of young fish in group C and group E were significantly higher than those in control group, and those in group B were significantly lower than those in control group (P<0.05), and the pepsin activities in liver of young fishes in group D were significantly lower than those in control group (P<0.05). The trypsin activity in liver of juvenile fish in group C was significantly higher than that in control group (P<0.05). The experiment indicates that the growth, body color and digestive enzyme indexes of red-fin Maylandia zebra juvenile are significantly affected by dietary supplementation, and the algae powder group has significant advantages in improving body color and improving digestive enzyme activity. [ABSTRACT FROM AUTHOR]

The experiment was to investigate the effect of different algae powder on digestive enzyme activity, antioxidant indexes and body composition of Acrossocheilius hemispinus. A total of 2 700 Acrossocheilius hemispinus were randomly divided into three groups, with three replicates in each group and 300 fish in each replicate. Acrossocheilius hemispinus in group 1 (control group) were fed with basal diet. The fish in group 2 were fed with basal diet supplement with 5% Chlorella, and the fish in group 3 were fed with basal diet with 5% Spirulina. The experiment lasted for 90 d. The results showed that compared with control group, the activity of fish intestinal digestive enzyme (AMS) in test group 2 and group 3 was significantly increased (P<0.05). There was no significant difference in intestinal lipase (LPS) activity among all groups (P>0.05). Compared with control group, the activity of trypsin (TRY) in group 2 and group 3 was significantly increased (P<0.05). Compared with control group, the serum total antioxidant capacity (T-AOC) in group 2 and group 3 was significantly increased (P<0.05), and the content of malondialdehyde (MDA) was significantly decreased (P<0.05). There was no significant change in whole fish nutrient composition among experimental groups (P>0.05). The experiment indicates that adding Chlorella or Spirulina in diet can significantly improve the activity of amylase and trypsin, improve the oxidation capacity of fish to a certain extent, but has little effect on the whole body composition, and the effect of adding Chlorella in diet is better than that of Spirulina. [ABSTRACT FROM AUTHOR]