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Copyright of Journal of Modern Laboratory Medicine is the property of Journal of Modern Laboratory Medicine Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Low-Carbon Chemistry & Chemical Engineering is the property of Low-Carbon Chemistry & Chemical Engineering Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Clinical Neurosurgery / Linchuang Shenjingwaike Zazhi is the property of Editorial Board of Journal of Clinical Neurosurgery and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Shandong Medical Journal is the property of Shandong Medical Health Newspapers and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Chinese Journal of Immunology is the property of Medical Periodical Society of Jilin and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Food & Machinery is the property of Food & Machinery Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Engineering Management & Technology Discussion is the property of Omniscient Pte. Ltd. and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Engineering Management & Technology Discussion is the property of Omniscient Pte. Ltd. and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Southwest China Journal of Agricultural Sciences is the property of Editorial Department of Southwest China Journal of Agricultural Sciences and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Modern Laboratory Medicine is the property of Journal of Modern Laboratory Medicine Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Henan Agricultural Sciences is the property of Editorial Board of Journal of Henan Agricultural Sciences and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Agro-Environment Science is the property of Journal of Agro-Environment Science Editorial Board and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Basic Sciences Journal of Textile Universities / Fangzhi Gaoxiao Jichu Kexue Xuebao is the property of Basic Sciences Journal of Textile Universities and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Chinese Journal of Cancer Biotherapy is the property of Editorial Office of Chinese Journal of Cancer Biotherapy and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Objective: To screen the disease genes and its related miRNA, and to study the interaction between the two and the role of genetic bone disease. Methods: This study applied the method of bioinformatics, firstly analyzed pathogenic gene of genetic bone disease by mirwalk and "international standard classification of genetic bone disease". According to the screening out of the pathogenic genes, Used mirwalk's 10 prediction miRNA search engine function, searched for the disease genes related to miRNA, and the excel work table was used to analyze the target genes of miRNA, cytoscape software was used to analyze the interaction between pathogenic genes and miRNA. Results: This study was closely associated with genetic bone disease genes can be divided into four categories: The first category was osteogenesis imperfecta type gene such as COL1A1, COL1A2, etc; The second category was the decreased bone density genes such as ACVR1, ALX1, etc; The third category was the increased bone density genes such as CASR, DMTF1, etc; The fourth category was functional backbone participates in bone density increase genes such as TGFBR1, MYCN, etc. With osteogenesis imperfecta which most closely mirna was hsa-miR-26b, hsa-miR-19, hsa-miR-200c, the hsa-miR-138 and hsa-miR-505 were the most closely related to the decreased bone density, the mirna with most closely related to the increased bone density was hsa-miR-196a, hsa-miR-200b, hsa-miR-19b. Conclusion: Mirna play an important role in the pathogenesis of genetic bone disease by regulating pathogenic genes,which show that the above mirna may become a new therapeutic target of prenatal screening and clinical drugs. [ABSTRACT FROM AUTHOR]

Copyright of Journal of Henan Agricultural Sciences is the property of Editorial Board of Journal of Henan Agricultural Sciences and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Chinese Journal of Bioinformatics is the property of Bioinformatics and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Objective To observe the effects of different concentrations of metformin on proliferation,apoptosis,invasion,and PTEN expression of human breast cancer cells,and to explore its mechanism. Methods The human breast cancer cell line SK-BR-3 was divided into four groups: 0 mmol/L group,5 mmol/L group,10 mmol/L group,and 20 mmol/L group with treatment of corresponding concentrations of metformin. CCK-8 was used to detect the inhibition rate of cell proliferation,Annexin V-FITC/PI double staining method was used to detect the apoptotic rate of each group,Transwell invasion test was used to detect the invasive ability of each group,q RT-PCR was used to detect the PTEN gene of each group,and Western blotting was used to detect the PTEN protein of each group. Results The inhibition rates of cell proliferation in the 0 mmol/L group,5 mmol/L group,10 mmol/L group,and 20 mmol/L group were 0.0% ± 0.0%,23.7% ± 2.2%,43.2% ± 2.9%,and 63.9% ± 5.6%,and the apoptotic rates were 5.1% ± 0.2%,18.2% ± 1.9%,26.3% ± 2.0%,and 40.1% ± 2.6%,respectively. The number of cell penetrating the membrane in the 0 mmol/L group,5 mmol/L group,10 mmol/L group and 20 mmol/L group were 170 ± 23,112 ± 14,74 ± 9,and 30 ± 5,respectively. The relative expression levels of PTEN mRNA in the 0 mmol/L group,5 mmol/L group,10 mmol/L group,and 20 mmol/L group were 1.01 ± 0.04,2.89 ± 0.27,4.12 ± 0.23,and 5.45 ± 0.49,and the relative expression levels of PTEN protein were 0.13 ± 0.05,0.41 ± 0.06,0.61 ± 0.10,and 0.94 ± 0.11,respectively. Significant differences were found in the above indexes between groups(all P < 0.05). Conclusion Metformin of 5,10,and 20 mmol/L can regulate the proliferation,apoptosis and invasion of human breast cancer cells. Metformin may regulate the proliferation,apoptosis and invasion of breast cancer cells by up-regulating the expression of PTEN. [ABSTRACT FROM AUTHOR]

Copyright of Basic Sciences Journal of Textile Universities / Fangzhi Gaoxiao Jichu Kexue Xuebao is the property of Basic Sciences Journal of Textile Universities and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Objective To explore the expression of microRNA (miRNA)-142 in the breast cancer tissues and cells, and its effect on invasion and metastasis of breast cancer cells. Methods Ninety-two breast cancer tissues and the corresponding para-carcinoma tissues were collected, and human breast cancer cell lines MCF-7, MDA-MB-231 and MDA-MB-468 , as well as the human normal mammary epithelial cell line IIBL-100, were cultured in vitro. The miR-142 expression in tissues and cells was detected by using qRT-PCR. MDA-MB-231 cells were divided into the observation group and control group, which were then transfected with miR-142 mimics and negative control sequence, respectively. Furthermore, miR-142 was detected by qRT-PCR, and the invasion and migration of the transfected cells were observed by Transwell invasion and migration assay. Results The relative expression of miR-142 in the breast cancer tissues was 0. 35 ±0.09, which was lower than that (1.02 ±0. 04) in the adjacent tissues (P <0. 01). The relative expression levels of miR-142 in the MCF-7 , MDA-MB-231 and MDA-MB-468 cells of breast cancer were 0. 53 ± 0. 05 , 0. 17 ± 0. 06, and 0. 19 ± 0. 08 , respectively, which were lower than that (1.01 ±0. 03) in the human normal mammary epithelial cells IIBL-100 (all P < 0. 01) , and the relative expression levels of miR-142 in the high invasive breast cancer cells MDA-MB-231 and MDA-MB-468 were also lower than those in the low invasive breast cancer MCF-7 cells (all P<0.01). After transfection, the relative expression of miR-142 in the MDA-MB-231 cells of the observation group was 32. 97 ± 1.89, which was higher than that in the control group (1.01 ±0.06) (P<0.01). In the experiment of migration and invasion, the number of penetrating cells in the observation group was 110 ± 15 and 52 ±9, respectively, which were lower than that of the control group [ (305 ± 27) and (148 ±16)] (both P<0.01). Conclusions The expression of miR-142 in the breast cancer tissues and cells decreases, and the expression of miR-142 in highly invasive breast cancer cells is lower than that of low invasive breast cancer cells. Up-regulating miR-142 level in breast cancer cells can inhibit the invasion and migration of cancer cells. [ABSTRACT FROM AUTHOR]

Copyright of Journal of Agricultural Science & Technology (1008-0864) is the property of Journal of Agricultural Science & Technology and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Shandong Medical Journal is the property of Shandong Medical Health Newspapers and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Southwest China Journal of Agricultural Sciences is the property of Editorial Department of Southwest China Journal of Agricultural Sciences and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Shandong Medical Journal is the property of Shandong Medical Health Newspapers and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Objective To analyze the risk factors of early acute kidney injury (AKI) after liver transplantation from donation after cardiac death(DCD) donor liver. Methods Clinical data of 184 donors and recipients undergoing liver transplantation from DCD donor liver were retrospectively analyzed. According to the incidence of early AKI, all participants were divided into the AKI and non-AKI groups. The patients in the AKI group were subject to AKI staging. Baseline data, preoperative, intraoperative and postoperative related parameters were statistically compared between two groups. The cumulative survival rate and clinical prognosis of patients in non-AKI group and AKI group with different staging were statistically analyzed by Kaplan-Meier curve analysis. Results Among 184 patients, 68 cases (37.0%) presented with early AKI after liver transplantation including 31 stage 1 AKI, 26 stage 2 AKI and 11 stage 3 AKI, mainly occurring within postoperative 3 d. Univariate analysis revealed that preoperative levels of albumin <35 g/L, preoperative levels of serum sodium ≤137 mmol/L, operation time>7.5 h, intraoperative hemorrhage volume>3 000 mL, intraoperative red cell infusion volume>15 U and intraoperative urine amount ≤100 mL/h were the risk factors of early AKI after liver transplantation (all P<0.05). Multi-variate Logistic regression analysis demonstrated that intraoperative red cell infusion > 15 U was an independent risk factor of early AKI after liver transplantation [odds ratio(OR) 1.061, 95% confidence interval(CI) 1.008-1.118, P=0.024]. Result of Kaplan-Meier survival curve suggested that the cumulative survival rate was gradually reduced along with the aggravation of AKI with statistical significance (all P<0.05). Conclusions The incidence of early AKI following liver transplantation is relatively high. The severity of early AKI is intimately correlated with the short- and long-term prognosis of the recipients. A large quantity of intraoperative red blood cell infusion is an independent risk factor of AKI. [ABSTRACT FROM AUTHOR]

Copyright of Shandong Medical Journal is the property of Shandong Medical Health Newspapers and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

In order to examine the exogenous situation of heavy metals in tobacco growing areas of Zunyi City, irrigation water samples(n=572) and organic fertilizer samples(n=21) were collected from the main tobacco growing areas. The concentration of As, Cd, Cr and Pb were investigated and compared with the national standards. The results showed that the concentration of As, Cd, Cr and Pb in the irrigation water were 0~71%, 0~2.9%, 0~6.6% and 0~8.5% of the national standard respectively. The concentration of As, Cd, Cr and Pb in fertilizers were 3.87%~64.1%, 2.33%~74.7%, 6.53%~38.1% and 10.9%~59.2% of the national standard respectively. The concentration of heavy metals in irrigation water and organic fertilizer in the main tobacco growing areas of Zunyi City is at the safe level. [ABSTRACT FROM AUTHOR]

[Objective]The study aims to investigate the feasibility of using color parameters to estimate the curing characteristics of fresh flue-cured tobacco leaves in maturity process. [Method] The color parameters of flue-cured tobacco leaves with different maturity were measured by color difference meter and curing characteristics were studied through the black box testing using flue-cured tobacco NCI 02 middle leaves. Cured-leaves were taken to verify the curing characteristics of different maturity fresh flue-cured tobacco leaves. [Result] The result shows that color values of front and back of flue-cured tobacco leaves had similar changing trend with the enhancement of leaves maturity. The relationship between color parameters(L ,b ,C) and maturity of tobacco leaves was statistically significant, which could be used to distinguish maturity. [Conclusion]The curing characteristics of flue-cured tobacco leaves with different maturity were different. The Fisher discriminant function based on color parameters can be used to identify different maturity leaves. Color parameters could be used as an auxiliary index to judge the curing characteristics of fresh flue-cured tobacco leaves. [ABSTRACT FROM AUTHOR]

In coder to explore the spectrum characteristics of tobacco leaf and its relationship with SPAD value in the field of different maturity, the differences of hyperspectral reflectance, location, area and vegetation variables of different maturity tobacco leaf were analyzed. Correlation analysis and stepwise regression method were used to determine the relationship between hyperspectral parameters and SPAD values. The results showed that with increasing maturity, the reflectance in the visible range (480-680 iim) gradually increased, especially in the 500 - 660 urn range for the reflectance of tobacco leaf maturity better distinguishing effect. In the near-infrared region, with increasing maturity, the reflectivity of tobacco generally showing a decreasing trend. Trend red edge area of tobacco is as follows: first and then decrease; blue border area with increasing maturity increasing; SDr / SDb value and (SDr - SDb) / (SDr + SDb) value decreases and the difference between the maturity reached significant or highly significant. The prediction effect of the regression equation of SPAD value of the Red Valley reflectance is better, and it is feasible to build a regression model based on the high spectral characteristic parameter to predict the SPAD value. [ABSTRACT FROM AUTHOR]

Copyright of Shandong Medical Journal is the property of Shandong Medical Health Newspapers and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Shandong Medical Journal is the property of Shandong Medical Health Newspapers and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)