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Copyright of Chinese Journal of Tissue Engineering Research / Zhongguo Zuzhi Gongcheng Yanjiu is the property of Chinese Journal of Tissue Engineering Research and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

BACKGROUND: Stress-tolerant multilineage differentiated (Muse) cells have excellent medical potential for regenerative medicine. However, at present, Muse cells need to be cultured and proliferated in vitro by suspension of cell aggregation, which is not conducive to large-scale expansion and application. Protein is one of the important components of extracellular matrix. Different proteins play different roles in cell growth. Therefore, the high-throughput method can be used to rapidly screen protein matrix that is conducive to the adhesion and proliferation of Muse cells on a two-dimensional surface. OBJECTIVE: To identify suitable protein substrates for surface adhesion and passaging of Muse cells. METHODS: Bovine serum albumin, bovine blood fibrin, and gelatin were used as raw materials, and their solutions were printed on glass slides by an ink jet printer and mixed on site to prepare protein microarray chips. The optimal protein combination for the adhesion of Muse cells was identified by using highthroughput screening approach. The Muse cells were cultured and passaged on the identified protein substrates for characterization. RESULTS AND CONCLUSION: (1) Two protein combinations were obtained by high-throughput screening, which were beneficial to the adhesion of primary Muse cells. They were bovine serum albumin/gelatin and bovine blood fibrinogen/gelatin, with combination ratios of 7/17 for both. (2) CCK-8 assay showed that Muse cells proliferated well in all groups. Among them, cells cultured on plates coated with bovine blood fibrinogen/gelatin proliferated well in 6 days. (3) By analyzing the immunofluorescence staining results of passages 1 and 3 of Muse cells cultured on the protein-coated plate, it was found that the combination of bovine blood fibrinogen and gelatin with a ratio of 7/17 in weight supported the adhesion and proliferation of Muse cells for at least 3 passages, maintaining good stem cell characteristics. [ABSTRACT FROM AUTHOR]

Copyright of Journal of Changzhou University (Natural Science Edition) / Changzhou Daxue Xuebao (Ziran Kexue Ban) is the property of Editorial Department of Journal of Changzhou University and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Ag-In-Zn-S(AIZS)quaternary quantum dots(QDs)were successfully synthesized via thermal decomposition method. By simply changing the amount of Zn precursor, the emission wavelength of the obtained QDs was tuned from 622 nm to 545 nm. After that, these hydrophobic QDs were subject to phase transfer via encapsulation by olaylamine grafted poly(isobutylene-alt-maleic anhydride)(PBMA-g-OAM). XRD, EDS and TEM were used to characterize the structure of AIZS QDs. Fluorescence spectrometer was used to measure the emission spectra of the QDs. FT-IR was used to confirm the formation of the PBMA-g-OAM amphiphilic copolymer. DLS was used to characterize the hydrodynamic size of AIZS/ PBMA-g-OAM nanospheres. SEM was used to demonstrate the morphology of the obtained AIZS/ PBMA-g-OAM nanocomposite. [ABSTRACT FROM AUTHOR]