1. Antibiotic and disinfectant resistance of Salmonella enterica Typhimurium
- Author
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Xueping HE, Xuebin XU, Aiping LIU, Li HE, Yong YANG, Xiaolin AO, Kang ZHOU, Shujuan CHEN, and Likou ZOU
- Subjects
salmonella enterica typhimurium ,antibiotic ,disinfectant ,resistance ,Food processing and manufacture ,TP368-456 ,Nutrition. Foods and food supply ,TX341-641 - Abstract
Objective The aims of this study were to investigate the antibiotic resistance, disinfectant resistance and their associations in 302 Salmonella enterica Typhimurium (S. Typhimurium) isolates from retail meats, food animals and humans. Methods Antibiotic susceptibility testing was performed according to the Kirby-Bauer disk diffusion method. The minimal inhibitory concentrations (MICs) of disinfectants were determined by broth microdilution method, and disinfectant resistance genes were detected by polymerase chain reaction (PCR) amplification. Results Antibiotic susceptibility testing demonstrated that 96.03% (290/302) of S. Typhimurium isolates were resistant to at least one antibiotic and 79.80% (241/302) were multidrug resistant (MDR). Tested isolates showed the highest resistance to streptomycin (78.81%, 238/302), followed by compound sulphonamides (78.15%, 236/302), tetracycline (75.50%, 228/302) and ampicillin (71.85%, 217/302). All isolates were sensitive to cefepime and imipenem. The resistance of S. Typhimurium from food to compound sulphonamides, tetracycline, ampicillin, nalidixic acid, and gentamicin were significantly higher than those from human and animal origin (P1 024 mg/L, respectively. In addition, the frequency of resistance to PVP-I, BC and TCS was observed significantly higher in food and animal isolates than human isolates (P
- Published
- 2020
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