1. [Effect of DRB/alpha-Amanitin on localization of Nrf2 in A549 cells].
- Author
-
Qu LY, Jiang YL, and Tang XW
- Subjects
- 20-Hydroxysteroid Dehydrogenases genetics, 20-Hydroxysteroid Dehydrogenases metabolism, Carcinoma, Non-Small-Cell Lung genetics, Carcinoma, Non-Small-Cell Lung metabolism, Carcinoma, Non-Small-Cell Lung pathology, Cell Line, Tumor, Heme Oxygenase-1 genetics, Heme Oxygenase-1 metabolism, Humans, Lung Neoplasms pathology, NF-E2-Related Factor 2 genetics, Alpha-Amanitin pharmacology, Dichlororibofuranosylbenzimidazole pharmacology, Lung Neoplasms metabolism, NF-E2-Related Factor 2 metabolism, Nucleic Acid Synthesis Inhibitors pharmacology
- Abstract
Objective: To investigate the effects of transcriptional inhibitors 5, 6-dichloro-1-b-D-ribofuranosylbenzimidazole (DRB) and alpha-Amanitin on the localization of Nrf2 in the nucleus., Methods: A549 cells were treated with DRB (50 mg/L) or alpha-Amanitin (2.5 mg/L)for 1 h and 6 h in serum-free medium, respectively. The expressions of Nrf2, HO-1, NQO1 and AKR1C were detected by Western blotting analysis. The localization of Nrf2 was determined by laser scanning confocal microscopy after cells were treated with either DRB or agr:-Amanitin for 1 h., Results: The expressions of Nrf2 and Nrf2-ARE gene batteries HO-1, AKR1C and NQO1 were decreased after 6 h treated with either DRB or alpha-Amanitin. The expression of SC35 was up-regulated but RNA Pol II was down-regulated; Y12 and NPC did not significantly change. The localization of Nrf2 in the cell nucleus did not change significantly., Conclusion: DRB and alpha-Amanitin can down-regulate the expression of Nrf2 and its targeting proteins HO-1, AKR1C and NQO1, but may have no effect on the localization of Nrf2.
- Published
- 2010
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