1. [Protein profiling of human dendritic cells infected with mycobacterium tuberculosis].
- Author
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Bao JL, Le J, Tian YP, Yang YP, and Wang HH
- Subjects
- Actins analysis, Annexin A4 analysis, Arsenite Transporting ATPases, Cell Line, Electrophoresis, Gel, Two-Dimensional, Heat-Shock Proteins analysis, Humans, Ion Pumps analysis, Multienzyme Complexes analysis, Protein Array Analysis, Proteomics, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Dendritic Cells immunology, Dendritic Cells microbiology, Mycobacterium tuberculosis immunology
- Abstract
The response of dendritic cells (DCs) plays an essential role in the initiation of immune responses following Mycobacterium tuberculosis (MTB) challenge. Two-dimensional electrophoresis (2-DE) was employed to compare the global protein patterns between human DCs infected and that uninfected with MTB H37Rv ATCC 27294 strains, and 45 protein spots were found to express differentially. Four protein spots which remarkably changed in DCs infected with MTB H37Rv ATCC 27294 strains were measured by matrix assisted laser desorption/ionization tandem time-of-flight (TOF/TOF) mass spectrometry. The data obtained from peptide mass fingerprinting were used in protein database search. Four protein spots in gel were identified as Human Arsenite-stimulated ATPase (hASNA-I), Annexin IV, gamma-actin and Heat shock protein27 (HSP27). These data provide insight into the changed global protein patterns of the DCs after infection and may prove useful for further study in the interaction between MTB and host.
- Published
- 2005