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Aiming at the shortcomings of current beam pumping units with small stroke，large energy consumption，and poor movement performance，as well as the general problem of low reliability and unstable transmission of beam less pumping units，a design scheme of the reversing device of a long stroke pumping unit which realizes the pure mechanical non-switching type reversing by mechanical characteristic is presented. The 2K-H planetary gear train is input in parallel through the anti-parallelogram mechanism and the gears，and the device commutation is realized under the specific transmission ratio of the parallel gears. The new type of commutator avoids the reliability problems of motor and chain commutator，as well as the vibration problems of non-circular gear commutator，and the transmission efficiency is improved compared with crank and rocker commutator. The movement analysis of the reversing device is carried out，and the multi-parameter calculation simulation is compared. The influence of each parameter on the movement performance of the device is analyzed. The results show that the downstroke time of the reversing device is shorter than that of the upstroke，which is helpful to improve the production efficiency. The mechanism parameters can be adjusted according to the actual work requirements to meet the requirements of working conditions，and have higher design compatibility in the actual application. The device can realize efficient and reliable reversing work，and has a wide range of application prospects in the future development of tower pumping units.

Objective Utilizing a specially engineered miR-144-GFP-H1 human embryonic stem cell (hESC) reporter line, this study leverages GFP fluorescence as an indicator of miR-144 expression to gauge the progression of erythropoiesis. The investigation is aimed at elucidating the potential roles of lncRNAs within the erythropoietic framework and conducting an initial assessment of their functional impact. Methods The miR-144/451-GFP-H1 cell line (hereafter referred to as 144-H1) was utilized for in vitro erythrocyte induction culture. The subpopulations of cells entering the erythropoiesis stage were characterized by the surface molecules CD71 and GPA. The GFP reporter gene of miR-144 served as a critical determinant to distinguish between GFP-positive cells (with a high propensity for erythropoiesis) and GFP-negative cells (with a low propensity for erythropoiesis). Transcriptome sequencing was performed on both groups to identify differentially expressed long non-coding RNAs (lncRNAs). LncRNA entries with potential for validation were selected for preliminary functional verification. The CRISPR/Cas9 gene editing technique was employed to design functional interference strategies for the targeted lncRNAs, obtaining 144-H1 cell lines with knocked-out function of the specific lncRNAs. These knockout cell lines, along with non-knockout 144-H1 cell lines, were used for parallel erythrocyte induction culture to identify differential nodes. This approach preliminarily verified their impact on erythropoiesis in an in vitro development model. Results 1)The constructed 144-H1 cell line was capable of expressing GFP fluorescence upon entering the stage of in vitro erythrocyte induction, indicating the activation of miR-144/451. 2)Within the CD71, GPA double-positive group, significant differences in lncRNA expression were observed between the GFP-positive and GFP-negative subpopulations. 3) Gene editing strategies involving the deletion of sequence segments capable of effectively interfering with the function of multiple lncRNA entries were designed and verified for successful editing. In the knockout cell lines, parts of the lncRNA sequences were directly deleted. 4)In parallel validation experiments of erythrocyte induction culture, cell lines with LINC01569 knocked out exhibited significant differences in flow cytometric subpopulations and cell proliferation capabilities compared to the non-knockout cell lines: ①The knockout cell lines showed sustained high expression of GFP fluorescence. ②The proportion of the CD71-GPA double-positive group in the knockout cell lines continuously decreased during erythrocyte maturation. ③No significant expression of hemoglobin was observed in the knockout cell lines, lacking the characteristic red color. ④The cell proliferation capability of the knockout cell lines was significantly lower than that of the non-knockout cell lines (P

To achieve a highly reliable and adaptive packet routing protocol in a complex urban Internet of vehicles, an end-edge-cloud edge intelligence architecture was proposed which consisted of an end user layer, an edge collaboration layer, and a cloud computing layer.Based on the proposed edge intelligence architecture, an packet routing protocol based on multi-intelligent reinforcement learning technologies was designed.The experimental results show that the proposed protocol could significantly improve the transmission delay and the packet reception rate in the interval of 29.65%～44.06% and 17.08%～25.38% compared to the state-of-the-art transmission mechanism for emergency data (TMED), intersection fog-based distributed routing protocol (IDR), and double deep Q-net based routing protocol (DRP).