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[Objective] Effects of POUlFl gene in three kinds of shear in Tibetan chicken in the different stages on the growth of the Tibetan chicken was studied.[Methods]Taken Tibetan chicken as the research object, using fluorescence quantitative technology, the expression a-mounts of the POUlFl three spliceosome of the pituitary, leg muscle and chest muscle in Tibetan chicken four growth periods (81, 119, 154, 210 days) were determined, and its association with growth trails was analyzed.[Results] The splicing variants of POUlFl gene all had a high expression level at 1 19 days in four periods and then decreased with increasing age; And their expression levels were in the order of POUlFl -β > POUlFl-α > POUlFl-ω; Among the pituitary, chest and leg muscle, the expression in pituitary was much higher than that of other two groups; In the pituitary gland, the expression of female was higher than that of male.The expression of POUlFl-α with 4 indi-cators (live weight, semi eviscerated weight, eviscerated weight and leg muscle weight) were significantly correlated and highly significantly correlated with the carcass weight; At the age of 119 days, POUlFl-α and POUlFl-ω were significantly correlated with the live weight, carcass weight, half eviscerated weight and leg muscle weight; At the age of 154 days, there was no significant correlation between POUlFl-ω and involved in the analysis of the characters.[Conclusion] The three spliceosome POUlFl gene before 119 days of age affecting on the growth and development of the chicken was the most significant, and POUlFl -α and POUlFl-β had a significant effect on the growth and development of Tibetan chicken, which could be used as candidate genes to improve livestock growth, increase the output capacity and provide a theoretical basis for livestock. [ABSTRACT FROM AUTHOR]

A high performance liquid chromatography with fluorescence detection method for simultaneous determination of five kinds of sulfonamide residues in chicken was developed. The sulfonamide residues were extracted from chicken samples with methylene chloride by microwave-assisted extraction technique, derivatizated with o -phthalalde-hyde and analyzed by high performance liquid chromatography with fluorescence detection. Five kinds of sulfonamide derivatizations were well separated within 8 minutes under the optimal separation conditions. Calibration curves were linear in the range of 0. 01 ~5 µmol/L (correlation coefficients above 0. 9902) with detection limits ranging from 1 to 10 nmol/L for the five analytes. The average recoveries were between 93. 3 % to 103. 3% with relative standard deviations less than 5. 03%. [ABSTRACT FROM AUTHOR]