Search

Plant cysteine proteases(CPs)widely affect plant physiological processes and disease resistance regulation. To study the functional mechanism of tobacco cysteine proteases in regulating potato virus Y(PVY), the CPs family genes of Nicotiana tabacum L. at the genome‑wide level were identifed. The evolutionary relationships, motifs and promoter cis‑acting elements of CPs family genes were analyzed by bioinformatics. Then the miRNAs corresponding to CPs were screend, the expression characteristics of CPs genes and their corresponding miRNAs after PVY infection were analyzed. The results showed that a total of 70 CPs genes were identified in Nicotiana tabacum L. genome, which were divided into five subfamilies, of which C1A contained 39 CPs, C2A contained 2 CPs, C12 contained 5 CPs, C13 contained 8 CPs and C14A contained 16 CPs. Motif analyses showed that the CPs of subfamily had similar motif distribution. Promoter cis‑acting elements analysis showed that several CPs family gene promoters contained light response elements, hormone response elements and low temperature, drought, high temperature, salt stress response elements. Based on the sequencing results of transcriptome and small RNA, it was found that 38 CPs were up‑regulated while 18 CPs were down‑regulated after PVY infection.Total 51 miRNAs were found to be targeted to 28 members of cysteine protease gene family, 38 miRNAs were up‑regulated while 13 were down‑regulated after PVY infection,7 of them were negatively correlated with miRNAs.The results of RT‑qPCR showed that 15 CPs genes were significantly up‑regulated after PVY infection. This study is conducive to better understanding the functions of CPs genes and their corresponding miRNAs in regulating tobacco PVY responses, which may provide evidence for the molecular mechanism of anti‑PVY in Solanaceae crops. [ABSTRACT FROM AUTHOR]

Copyright of Journal of China Medical University is the property of Journal of China Medical University Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Acta Agronomica Sinica is the property of Crop Science Society of China and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Southern Agriculture is the property of Journal of Southern Agriculture and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Objective: To investigate the mechanism of the effect of acupuncture and moxibustion on improving liver injury by observing the changes of cysteine protease (Caspase) associated with hepatocyte apoptosis based on cisplatin (DDP) induced liver injury model mice., Methods: Forty KM mice were randomly divided into control group, model group, acupuncture group and moxibustion group, with 10 mice in each group. The liver injury model was replicated by intraperitoneal injection of DDP. In the acupuncture group and the moxibustion group, acupuncture and moxibustion were performed at"Dazhui"(GV14), and bilateral "Ganshu"(BL18), "Shenshu"(BL23), and "Zusanli"(ST36), respectively, once per day for 5 d. General condition of mice in each group were observed;The activities of AST, ALT and GLDH in mice serum were detected by biochemical method. ELISA and Western blot assay were used to detect Caspase-3, Caspase-8 and Caspase-9 contents and protein expression in the liver tissues of each group of mice, respectively., Results: Compared with the control group, the general condition of the mice in the model group was poorer, and the Caspase-3, Caspase-8 and Caspase-9 contents and protein expressions in liver tissues and the activities of AST, ALT and GLDH in serum were increased ( P <0.05). Compared with the model group, the general condition of the mice in the acupuncture and moxibustion groups improved, and the Caspase-3, Caspase-8 and Caspase-9 contents and protein expressions in liver tissues and activities of AST, ALT and GLDH in serum were decreased ( P <0.05)., Conclusion: Acupuncture and moxibustion can reduce liver injury due to DDP chemotherapy by modulating the expression of apoptotic factors Caspase-3, Caspase-8 and Caspase-9 in liver tissues of DDP model mice and improving liver function, which may be one of the mechanisms of the effect of acupuncture and moxibustion to ameliorates liver injury after DDP chemotherapy.