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Copyright of Journal of Food Safety & Quality is the property of Journal of Food Safety & Quality Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

The use of antibiotic can influence gut microbiota and impair memory function either positively or negatively. In order to investigate the effects of long-term exposure to doxycycline at environmental concentration on anxiety behavior, learning and memory, cognitive flexibility and intestinal flora, we took zebrafish as the animal model, and used doxycycline at different environmental concentrations (0, 0.01, 0.1 and 1 μg/L) to expose adult zebrafish to water for 21 days. Behavioral responses and intestinal flora alterations were assessed using the new tank test, Y-maze, light and dark test and zebrafish behavior high-throughput monitoring system. The results showed as follows: in the new tank experiment, the average swimming speed of zebrafish in 0.01, 0.1 and 1 μg/L concentration groups decreased significantly compared with the control group. Additionally, higher doxycycline concentrations correlated with reduced maximum swimming speed and total swimming distance, with a pronounced difference observed in the 1 μg/L group. Maze experiments indicated a significant increase in the residence time of zebrafish in the exploration arm at the 0.01 μg/L concentration, contrasting with decreased times in the 0.1 and 1 μg/L groups. The turning angle exhibited a similar pattern, increasing in the 0.01 μg/L group but decreasing in the higher concentration groups. In the light and dark test, zebrafish demonstrated an increased residence time in the light zone with rising doxycycline concentration. Zebrafish behavioral high-throughput monitoring system experiments further confirmed a significant decrease in total swimming distance with increasing doxycycline concentration, particularly notable in the 1 μg/L group. In terms of intestinal flora analysis, based on 16S rRNA sequencing technology, 1454814 original valid sequences were obtained from 12 intestinal samples, and 1314404 high-quality sequences were obtained after screening for subsequent analysis. At the same time, DADA2 method was used to reduce noise and cluster with 100% similarity. It was found that the abundance of intestinal flora of zebrafish in each concentration group decreased, and the abundance of bacteria genera such as Bosteria, Paracococcus and Acinetobacter decreased, while genera like Corynebacterium and Romatococcus increased. The Shannon index, Simpson index and Chao1 index were significantly lower in the exposed group, indicating decreased diversity. In conclusion, long-term exposure to doxycycline in environmental concentration induced behavioral changes in zebrafish, resulting in anxiety-like behavior. The 0.01 μg/L concentration improved short-term learning and memory ability and cognitive flexibility. Furthermore, doxycycline exposure led to changes in the abundance and diversity of zebrafish intestinal flora. [ABSTRACT FROM AUTHOR]

Objective To observe the clinical efficacy and safety of Shuangshi Tonglin capsules combined with doxycycline hydrochloride in treating male non-gonococcal urethritis (Damp-Heat Pouring Downward type). Methods From March 2022 to March 2023, 104 patients with non-gonococcal urethritis (Damp-Heat Pouring Downward type) were randomly divided into two groups based on block randomization, including the treatment group (n =52) and the control group ( n = 52) . The patients in the control group were orally administrated with doxycycline hydrochloride for 7 days, and the treatment group with Shuangshi Tonglin capsules in combination with doxycycline hydrochloride for 7 days. Both groups of patients were re-examined at the 7th day after stopping the medication. The clinical efficacy of the two groups was observed and compared, as well as the traditional Chinese medicine symptom scores, the time of clinical symptom regression, laboratory test results for the pathogen, and the incidence of adverse reactions. Results The total efficacy rate for the treatment group and the control group was 96. 15% and 84. 62%, respectively, with a significant difference (P <0. 05) . Symptom regression times of the treatment group for frequent urination, urgency, pain, redness and swelling of the urethral opening, itching, and urethral discharge[ (3.12 ±0.38), (3.34 +0.44), (4.12 ±0.68), (4.03 ±0.59), (3.36 ±0.32), and (3.56 ±0.52) days] were all better than those of the control group[ (4.26 ± 0.76), (4.76 ±0.83), (4.89 ±0.89), (5.12 ±0.92), (4.56 ± 0.82), and (4.64 ±0.78) days], with statistical significance (P <0. 05) . Symptom scores of both groups after treatment showed an obvious decrease (P <0.05), and the scores for the treatment group for each symptom [(0.86 ±0.18), (0.94 ± 0.23), (0.79 ±0. 6), (1.01 ±0.27), (0.83 ± 0.20), and (0.97 ±0.25)] were all notably lower than those of the control group[ (1.38 ±0.46), (1.54 ± 0.52), (1.46 ±0.49), (1.50 ± 0.51), (1.44 ± 0.43), and (1.32 ± 0.36)], with a significant difference (P <0. 05) . The pathogen clearance rate of the treatment group was 96. 15%, which was higher than that of in the control group (82. 69%, P <0.05) . The cases with gastrointestinal reactions, headaches, maculopapular rash, and dry mouth in the treatment group were 1, 1, 0, and 1 and the cases in the control group were 1, 2, 2, and 0 cases. The incidence of adverse reactions in the treatment group was 5.77%, which was lower than that in the control group. However, the difference was not statistically significant (P >0.05). Conclusions The combined treatment of Shuangshi Tonglin capsules and doxycycline hydrochloride for male non-gonocoecal urethritis of the Damp-Heat Pouring Downward type can significantly enhance the clinical pathogen clearance rate, effectively improve clinical symptoms and the life quality of patients. [ABSTRACT FROM AUTHOR]

Objective To explore the application of laparoscopic-assisted sclerotherapy for giant retroperitoneal lymphatic malformation (rLM). Methods From July 2020 to July 2021, retrospective review was conducted for clinical data of 3 children with giant retroperitoneal lymphatic malformation receiving laparoscopic-assisted doxycycline injection. And the relevant literature was retrived for discuss the approaches of injection therapy, efficacy and cautions. Results There were 1 boy and 2 girls with an age range of (26-68) month. Two cases presented with abdominal pain and inguinal masses. A total of 5 injections were performed and 2 cases treated twice. In case No.1, the maximal diameter of lesion decreased from 14.7 to 4.0 cm after treatment with a volume reduction of 95. 2%; In case No.2, the maximal diameter of lesion dropped from 11.8 to 5.5 cm after treatment with a volume reduction of 95%; In case No. 3, the maximal diameter of lesion declined from 9. 0 to 2.5 cm after treatment with a volume reduction of 98.9%. All of them reached the standard of cure and the symptoms disappeared. Only one case of postoperative vomiting resolved spontaneously. No recurrence or other complications were observed during a follow-up period of (6-12) months. Conclusions Sclerotherapy injection for gaint retroperitoneal lymphatic malformation has a marked efficacy and a high safety. And laparoscopic assistance may offer minimal invasion, improve accuracy of injection and reduce complications. [ABSTRACT FROM AUTHOR]

Objective To report a case of neurosyphilis complicated with Rickettsia felis infection, and summarize the clinical manifestations, cerebrospinal fluid (CSF) and imaging characteristics. Methods and Results A 33-year-old male with a disease course of more than 3 months was admitted to Beijing Tiantan Hospital, Capital Medical University on October 12, 2022, who had kept a cat at home for more than 10 years. The main clinical manifestations were seizure, cognitive function decline, and low fever in the last one month. Serum Treponema syphilis (TP) antibody, Treponema Pallidum Hemagglutination Assay (TPHA), Toluidine Red Unheated Serum Test (TRUST) were positive. CSF test showed increased white blood cell count and protein, positive oligoclonal band, 24 h intrathecal IgG synthesis rate and IgG index. Head MRI showed T2WI and FLAIR hyperintensity in bilateral medial temporal lobe and hippocampus atrophy, evident on the right side. There were 229 Rickettsia felis sequences and 2 TP sequences detected in CSF metagenomic next-generation sequencing (mNGS). After his medical history was asked again, he was infected with syphilis 7 years ago, and his serum and CSF were positive for TP antibody. The comprehensive clinical and imaging findings were consistent with the characteristics of neurosyphilis, and the fever was consistent with the characteristics of Rickettsia felis infection. He was diagnosed as neurosyphilis complicated with Rickettsia felis infection, who improved after penicillin and doxycycline treatment. Conclusions Neurosyphilis complicated with Rickettsia felis infection is clinically rare. mNGS can help with early diagnosis. Early anti-infective therapy can improve the prognosis. [ABSTRACT FROM AUTHOR]

Objective: To investigate the effects of doxycycline on in vitro osteogenic differentiation of pro ⁃ osteoblasts cell line MC3T3⁃E1 and the possible mechanisms. Methods: Alizarin red staining was employed to detect the osteogenic differentiation. Real⁃ time PCR was used to detect the effect of DOX on the expression of OCN and Runx2 in MC3T3⁃E1 cells. MC3T3⁃E1 cells were treated with either DOX，MEK inhibitor U0126 alone or in combination, and the levels of N⁃cadherin，p⁃MEK and p⁃ERK were detected by Western blot. Results: When DOX was added during osteogenic induction, alizarin red staining positivity of the cultured MC3T3⁃E1 was significantly enhanced. DOX increased the expression of OCN and Runx2 in MC3T3⁃E1 cells. Besides, DOX decreased the levels of N ⁃ cadherin and increased the level of p ⁃MEK and p ⁃ ERK in MC3T3 ⁃ E1 (P < 0.05). On the contrary, MEK antagonist U0126 significantly increased the expression of N⁃cadherin protein and decreased the levels of p⁃MEK and p⁃ERK (P < 0.05). When MC3T3⁃E1 cells were treated with DOX in the presence of MEK inhibitor U0126，the changes in N⁃cadherin, p⁃MEK and p⁃ERK were showed to be reversed comparing with DOX alone. When both U0126 and DOX were present during in vitro osteogenic differentiation of MC3T3⁃E1， alizarin red staining positivity was less observed than that of DOX alone. Conclusion: In this study, DOX is showed to enhance the in vitro osteogenic differentiation of MC3T3⁃E1，which is probably associated with MEK/ERK signaling pathway. [ABSTRACT FROM AUTHOR]

Copyright of Chinese Journal of Preventive Veterinary Medicine / Zhongguo Yufang Shouyi Xuebao is the property of Chinese Journal of Preventive Veterinary Medicine Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Environmental Science & Technology (10036504) is the property of Editorial Board of Environmental Science & Technology and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Northwest A & F University - Natural Science Edition is the property of Editorial Department of Journal of Northwest A&F University (Natural Science Edition) and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Food Safety & Quality is the property of Journal of Food Safety & Quality Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Objective To explore the antibacterial effect of artemether on P. acnes in vitro and its effect on the bacterial morphology in order to provide a rationale for its use in the treatment of acne. Methods The minimal inhibitory concentrations (MIC) of artemether, doxycycline hydrochloride and their combination on P. acnes were determined by broth microdilution method, while the morphological changes of P. acnes were observed with a scanning electron microscope. Results The MICs of artemether and doxycycline hydrochloride were (117.19±76.15) μg/mL and (0.08±0.05) μg/mL, respectively. In the group of P. acnes treated with combination of artemether and doxycycline, MICs of artemether and doxycycline were (31.96±19.58) μg/mL and (0.02±0.01) μg/mL, respectively. The MIC of artemether differed significantly between artemether treatment alone and artemether combined with doxycycline (t=4.03, P

Copyright of Journal of Food Safety & Quality is the property of Journal of Food Safety & Quality Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Objective: The objective of this retrospective study was to compare the efficacy of azithromycin versus doxycycline in the treatment of urogenital Chlamydia trachomatis infection. Methods: A retrospective study was conducted on all patients who tested positive for Chlamydia in our clinic from May 2017 to May 2019. Patients who did not follow the standard dose and course of treatment, did not receive the test-of-cure, or received other drugs that may affect the efficacy were excluded. Results: Of the 68 patients who initially received a 1 g single dose azithromycin, the total microbiological cure rate was 79.4% (54/68), while the microbiological cure rate in 19 patients who received doxycycline treatment was 100% (19/19). Use of drugs from different manufacturers and shorter time after completion of treatment to perform test-of-cure might lead to the possibility of underestimation of microbiological cure rate. Conclusions: These results suggest that 7-day doxycycline should be recommended as the 1st line treatment for urogenital Chlamydia infection. Considering the convenient dosage, ong-gram single dose of azithromycin could be recommended as an alternative treatment for urogenital Chlamydia infection.

Copyright of Chinese Journal of Preventive Veterinary Medicine / Zhongguo Yufang Shouyi Xuebao is the property of Chinese Journal of Preventive Veterinary Medicine Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Chinese Journal of Preventive Veterinary Medicine / Zhongguo Yufang Shouyi Xuebao is the property of Chinese Journal of Preventive Veterinary Medicine Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of South China Agricultural University is the property of Gai Kan Bian Wei Hui and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Henan Agricultural Sciences is the property of Editorial Board of Journal of Henan Agricultural Sciences and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Food Safety & Quality is the property of Journal of Food Safety & Quality Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Modern Laboratory Medicine is the property of Journal of Modern Laboratory Medicine Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Agro-Environment Science is the property of Journal of Agro-Environment Science Editorial Board and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

BACKGROUND: Doxycycline has a certain effect on disc degeneration progression, but the mechanism by which doxycycline inhibits the expression of matrix metalloproteinases (MMPs) in intervertebral disc remains unclear. OBJECTIVE: To clarify the mechanism by which doxycycline inhibits MMPs expression in intervertebral disc by cytologic experiment in views of intramolecular signal transduction. METHODS: The human nucleus pulposus cells were cultured in vitro, and 45 000 fibronectin fragments were added into the culture medium to make the degeneration model. The degenerative models were divided according to different action time and different concentrations. The expression levels of type II collagen and MMP13 in each group were detected by western blot assay. After treatment with 10 mg/L doxycycline for 24 hours, the expression levels of ERK1/2, p-ERK1/2, P38, p-P38, JNK, and p-JNK proteins in each group were determined by western blot assay. Based on the above experimental results, the inhibition of ERK and JNK pathways was selected, and the effect of doxycycline on the expression of MAPK pathway protein was detected by western blot assay. RESULTS AND CONCLUSION: Fibronectin could induce the degeneration of nucleus pulposus cells, showing an increase in expression level of MMP13 and a decrease in expression of type II collagen. Doxycycline could inhibit the expression of MMP13 in degenerative nucleus pulposus cells induced by fibronectin, but not in a time- or dose-dependent manner. After degenerative nucleus pulposus cells induced by fibronectin were treated with doxycycline, the phosphorylation of ERK1/2 and JNK was inhibited, and dual inhibition effectively blocked the activation of ERK1/2 and JNK compared with MAPK inhibitors or doxycycline alone. So doxycycline may block ERK1/2 and JNK activation in the nucleus pulposus cells to inhibit MMP expression, and thus reduce the extracellular matrix degradation. Thereafter, doxycycline exerts a potential role in preventing and treating disc degeneration. [ABSTRACT FROM AUTHOR]

Objective To observe the influence of Bugan Jianyao Fang (Liver-tonifying Lumbar-invigorating Decoction) on expressions of intervertebral disc matrix metalloproteinase-3 (MMP-3) and tissue inhibitor of metalloproteinase-1 (TIMP-1) in rats with lumbar disc degeneration. Methods SD rats (n=75) were randomly divided into sham-operation group, model group, Yaobitong (Waist Impediment-Freeing Capsules) group, doxycycline (DOX) group and Bugan Jianyao Fang group (each n=15). After establishing successfully the model of lumbar disc degeneration in rats, all groups were, respectively, given orally Yaobitong, DOX and Bugan Jianyao Fang. The expressions of intervertebral disc MMP-3 and TIMP-1 were detected in all group after and 20 d and 4 d after intervention. Results After intervention for 20 d and 40 d, the expressions of intervertebral disc MMP-3 and TIMP-1 decreased in Yaobitong group, DOX group and Bugan Jianyao Fang group compared with model group (P<0.05). The difference in expressions of intervertebral disc MMP-3 and TIMP-1 had statistical significance in Yaobitong group, DOX group and Bugan Jianyao Fang group after intervention compared with those in the same groups before intervention (P<0.05). The comparison among Bugan Jianyao Fang group, Yaobitong group and DOX group showed that the difference had no statistical significance (P>0.05) at all time points. Conclusion Bugan Jianyao Fang can reduce the expressions of intervertebral disc MMP-3 and TIMP-1 in rats with lumbar disc degeneration, which may be one of mechanisms of Bugan Jianyao Fang preventing and curing lumbar disc degeneration. [ABSTRACT FROM AUTHOR]

Copyright of Chinese Journal of Veterinary Science / Zhongguo Shouyi Xuebao is the property of Chinese Society of Veterinary Science and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Scrub typhus is a mite-borne bacterial infection of humans that is caused by Orientia tsutsugamushi, which causes generalized vasculitis. The disease may involve the tissues of any organ system but no case with involvement of the lower gastrointestinal tract has been reported. We report a case of a 39-year old Korean male with enterocolitis of severe scrub typhus, of which the serotype was Ikeda strain. The patient was admitted to hospital with fever, abdominal pain and shock. He developed multi organ failure and frequent watery diarrhea. Abdominal computed tomography revealed diffuse edematous thickening of the entire small and colon with inflammation. Three days after admission, the antibody to O. tsutsugamushi was reported to be 1:320. He improved with doxycycline and azithromycin, and the persistent watery diarrhea stopped at 24 hours. This study shows that scrub typhus should be considered when the small and large intestine are affected. For the genotype of O. tsutsugamushi in Korea, additional studies of the impact of changes in the vector distribution on the genotype distribution will be needed. [ABSTRACT FROM AUTHOR]

Objective: To investigate the expression of matrix metalloproteinase-2 (MMP-2) and matrix metalloproteinase-9 (MMP-9) in multiple myeloma (MM) patients, and analyze the effect of doxycycline (DOX) on the expression of MMP-2 and MMP-9 in MM cells., Methods: The peripheral blood and bone marrow samples of MM patients were collected, and the patients were divided into three groups: newly diagnosed group, remission group and relapsed/refractory group, while the peripheral blood samples of 34 health people and the bone marrow samples of 17 IDA patients were selected as normal control and control group. The levels of MMP-2 and MMP-9 were detected by ELISA. The protein levels of MMP-2 and MMP-9 in H929 cells treated by different concentrations of DOX were analyzed by Western blot. After H929 cells was treated by Akt inhibitor MK-2206 2HCl in combination with DOX, Western blot was used to detect the levels of MMP-2 and MMP-9., Results: The levels of MMP-2 and MMP-9 in newly diagnosed MM patients were higher than those in control (P<0.05), while for the patients in the remission group were decreased, but still higher than those in control. The levels of MMP-2 and MMP-9 were increased again for the patients in relapsed/refractory group, and showed no significant difference as compared with those in newly diagnosed group. The levels of MMP-2 and MMP-9 could be inhibited by 10 mg/L and 15 mg/L DOX treated by H929 cell. The protein levels of MMP-2 and MMP-9 showed no altered in H929 cells treated by 5 nmol/L MK-2206 2HCl alone. DOX exerted more profound inhibitory effect to MMP-2 and MMP-9 expression in H929 cells when Akt inhibitor MK-2206 2HCl was combined with DOX., Conclusion: The levels of MMP-2 and MMP-9 are increased in MM patients and related to the disease status of MM. DOX can inhibit the expression of MMP-2 and MMP-9 in MM cells, and antagonizing its activation of Akt signaling pathway can further enhance the inhibitory effect.

Objective: To investigate the mechanism of the in vitro toxicity of doxycycline to myeloma cell line H929 and also the possible pathway involved its toxicity., Methods: Myeloma cell line H929 was treated with DOX, MEK inhibitor U0126 or RAS agonist ML-098, either alone or in combination. Then, the expression of p-MEK, caspase-3, caspase-9 and c-Jun in H929 were used to detected by Western blot; the cells proliferation and apoptosis were detected by CCK-8 assay and flow cytometry, respectively., Results: DOX significantly increased the levels of cleaved caspase-3 and caspase-9, and down-regulated the level of p-MEK in H929 (P<0.05). MEK antagonist U0126 significantly increased the levels of cleaved caspase-3 and caspase-9, and down-regulated the level of p-MEK (P<0.05). After Dox combined with ML-098 treatment of H929 cells, the apoptosis rate of H929 cells was lower than that of DOX alone treatment group(P<0.05). Compared with DOX alone treatment group, the expressions of p-MEK and p-ERK1/2 in DOX+ML-098 combined treatment group were increased, and the levels of cleaved caspase-3,9 in H929 cells were decreased (P<0.05). The levels of c-Jun mRNA and protein increased in H929 when treated by DOX alone (P<0.05)., Conclusion: DOX can induce apoptosis of H929 via intrinsic apoptosis pathway, and MEK/ERK pathway and c-Jun possibly play a role in this process.

Objective To investigate the effects of doxycycline on the expressions of matrix metalloproteinase- 9 (MMP-9) and tissue inhibitor of metaloproteinase-1 (TIMP-1) in breast cancer lung metastasis model mice (BCML-TA II 99), and the mechanism thereof. Methods The BCML-TA II 99 mouse model was established. The immunohistochemical staining was performed to detect the expressions of MMP-9 and TIMP-1 in BCML-TA II 99 model mice and controls. Results The metastasis rate was significantly lower in treatment group than that of control group. The expression of MMP-9 was significantly reduced, but the expression of TIMP-1 was significantly increased, in treatment group compared with those of control group (P < 0.05). Conclusion Doxycycline can inhibit tumor growth and metastasis in breast cancer with 1ung metastasis model mice by regulating MMP-9 and TIMP-1 expressions. [ABSTRACT FROM AUTHOR]

Objective To investigate the effect of Doxycycline on gastric cancer cell line BGC-823 proliferation and Notch1, Matrix metalloproteinase-9(MMP-9)expression in order to shine a light on new anticancer drugs. Methods Final concentration of 0, 5, 10, 20, 40 mg/L doxycycline were added into human gastric cancer cell line BGC-823. Cell proliferation was detected by MTT; Cell cycle distribution was assessed by flow cytometry; Notch1, MMP-9 protein expression was revealed by Immunoblot. Results Doxycycline can inhibit proliferation of human gastric cancer cells line BGC-823, and its effect is dose and time-dependent(P<0.01). Doxycycline alters distribution of gastric cancer cell line BGC-823. With increasing drug concentration, the proportion of cells in S phase dropped(P<0.01). Notch1 expression rose and MMP-9 expression decreased(P<0.01). Conclusion Doxycyclinecan inhibited gastric cancer cell line BGC-823 proliferation and up-regulating Notch1 might be one mechanisms [ABSTRACT FROM AUTHOR]

Objective: The research is to study the expression and distribution of matrix metalloproteinase (MMPs)-2 and -9 in the guinea pig cochlea after noise exposure, and to explore the role of MMPs in the blood-labyrinth-barrier (BLB). In addition, the role of MMPs inhibitor doxycycline in noise-induced BLB trauma was studied as well, which provides the basis for further studies and prophylaxis of noise-induced hearing loss. Methods: A total of 45 healthy adult guinea pigs were randomly divided into the control group (15 received intraperitoneal injection of 0.9% saline for 4 consecutive days), the noise-exposure group (15 exposed by 120 dB SPL white noise for 4 h per day for continuous 2 d, intraperitoneal injection of normal saline for 4 consecutive days) and the noise-exposure + doxycycline group (15 exposed by 120 dB SPL white noise exposure for 4 h per day for 2 consecutive days, and intraperitoneal injection of doxycycline 50 mg/kg/d for 4 consecutive days), respectively. Immunofluorescence staining, western blot, and real-time quantitative PCR were used to analyze the distribution and differential expression of MMP-2 and -9 in the stria vascularis of guinea pigs in comparison with the normal control group, noise only group, and noise & doxycycline treatment group. Immunofluorescence staining was used to observe the changes in tight junction (TJ) protein ZO-1 in stria vascularis in three groups and to investigate the effect of acoustic injury on TJs. And ABR tests were utilized to detect the hearing function of guinea pigs in the three groups. Intravenous Evans blue was administrated intravenously as an indicator of vascular leakage among three groups to study the changes in BLB permeability in context of acoustic injury. SPSS 22.0 was used for statistical analysis. Results: There was no significant difference in hearing function between the noise-exposure group and the noise & doxycycline group two hours after noise exposure. After seven, 14 and 28 days noise exposure, the hearing recovery of the noise & doxycycline treatment group was significantly greater than that of the noise-exposure group ( P <0.05) . Immunofluorescence staining showed that there was only a small amount of MMP-2 and -9 in the stria vascular in the normal control group, and ZO-1 showed dense linear expression. While, in the noise-explore group, MMP-2 and -9 in the stria vascular was significantly elevated ( P <0.05), and the configuration of ZO-1 became loose and discontinuous. However, the MMP-2 and -9 in the noise & doxycycline treatment group were not significantly different from the normal control group ( P >0.05), which were significantly less than that in the noise-exposure group, and just a little break of ZO-1 was observed, however, the overall structure remained dense. The leakage of Evans blue from stria vascular capillary in the noise-exposure group was significantly increased, and the difference between the other two groups did not show any statistical significance ( P >0.05). Conclusions: The damage of tight junction structure induced by MMP-2 and -9 may play an important role in BLB destruction. In addition, doxycycline can inhibit MMPs secretion, thereby, to some extent, protecting the integrity of BLB from acoustic injury, and contributing to the long-term hearing recovery.

Purpose: To evaluate the treatment effect of topical doxycycline on avulsed permanent teeth compared with normal saline., Methods: A total of 44 avulsed teeth from 38 patients (22 boys and 16 girls, aged 7-14 years) were recruited. Twenty-one teeth in group A were treated with doxycycline for 5 min before replantation while 23 teeth in group B were treated with saline solution. All participants were followed up for at least 12 months. The clinical outcome differences between 2 groups was evaluated by Mann-Whitney U test., Results: In group A, 18 teeth were found pulp necrosis, 3 with infection-related resorption, 13 with ankylosis-related resorption and 6 were extracted. In group B, 16 were diagnosed with pulp necrosis, 4 with infection-related resorption, 12 with ankylosis-related resorption and 7 were extracted. No significant differences were found between the two groups on pulp survival and periodontal healing., Conclusions: Compared with treatment with normal saline, avulsed permanent teeth treated with doxycycline did not show a better clinical outcome.

Objective: To investigate the possible mechanism of doxycycline inhibiting paraquat-induced pulmonary fibrosis and provide a theoretical basis for its clinical application. Methods: Human lung fibroblast HFL1 cells were selected as the research object in the cell group. Divided into blank group, paraquat group, paraquat+doxycycline group. The expression of TGF-β1, a-SMA, Smad3 and Smad2 protein was detected by ELISA using 40 ml of paraquat 40 umol/L and 3 mg/L of oleic acid 10 mg/L. In the animal group, 120 healthy and clean SD rats were randomly divided into three groups: blank group, paraquat group, paraquat+doxycycline group. The expression of TGF-β1, a-SMA, Smad3 and Smad2 protein in lung tissue of mice at 1 day, 3 days, 7 days, 14 days and 21 days was detected by Elisa method. The expression of TGF-β1, a-SMA, Smad3 and Smad2 protein in lung tissue of 21-day mice was detected by Western Blotting. The pathological changes of lung tissue were observed by HE staining for 1 day, 3 days, 7 days, 14 days and 21 days. Results: In the cell group experiment, the expression of TGF-β1, a-SMA, Smad3 and Smad2 protein increased gradually with paraquat in the paraquat group, and the expression of TGF-β1, a-SMA, Smad3 and Smad2 protein was significantly higher than that in the blank group. The difference was statistically significant ( P <0.05) . The expressions of TGF-β1, a-SMA, Smad3 and Smad2 in the paraquat+doxycycline group were significantly lower than those in the paraquat group, but still higher than the blank group, the difference was statistically significant ( P <0.05) . Conclusion: Doxycycline inhibits paraquat-induced pulmonary fibrosis by inhibiting the expression of TGF-β1, a-SMA and Smad3, Smad2 proteins.

Purpose: To evaluate the therapeutic effects of doxycycline nano-liposome slow-release gel on the periodontitis in an established rat model., Methods: The biocompatibility was tested by oral perfusion sample gel for long term observation. Doxycycline nano-gel was used to treat the established rat model of periodontitis. The rats were selected randomly and divided into five groups: blank group, doxycycline nano-gel treated group, minocycline-treated group, matrix group and periodontitis model group. MMP-8 and TIMP-1 in the sulcus fluid were detected by real-time PCR. The data was analysed using SAS13.3 software package for Student's t test., Results: Doxycycline nano-gel had excellent biocompatibility from weight measure and tissue section evaluation. The results in periodontitis rats demonstrated that doxycycline nano-gel could ameliorate periodontitis condition dramatically compared with the control group, the restoration even better than minocycline-treated group., Conclusions: Doxycycline nano-liposome slow-release gel improves rat periodontitis by decreasing MMP-8 level, which provides valuable evidence to promote doxycycline nano-gel clinical application.

Objective: To investigate the effect of PTEN tumor suppressor gene combined with doxycycline on telomerase activity in human mucoepidermoid carcinoma cell line., Methods: The wild-type PTEN tumor suppressor gene or empty vector was introduced into mucoepidermoid carcinoma cell line in vitro, then the cancer cells were treated with doxycycline. Cancer cell survival was determined by MTT assay. Telomerase activity was determined using telomerase repeat amplification protocol-enzyme-linked immunosorbent assay (TRAP-ELISA)., Results: Compared to the control cells, cancer cells transfected with the wild-type PTEN gene showed growth inhibition and increased sensitivity to doxycycyline, and the ratio of augment of drug sensitivity was 1.65-4.75. The telomerase activity in cancer cells treared with PTEN gene transfection or doxycycline alone decreased, however, telomerase activity in combined group decreased more remarkably., Conclusion: PTEN gene in combination with doxycycline has significant inhibitory effect on telomerase activity in cancer cells.

Objective: To evaluate the ability of Biopure MTAD in removing smear layer in vitro., Methods: Forty extracted single-rooted human teeth were randomly divided into five groups: Distilled water in group A, 5.25% NaClO and 17% EDTA in group B, 1.3% NaClO in group C, 1.3% NaClO and Biopure MTAD in group D, 1.3% NaClO and 3% EDTA in group E. The canals were prepared and irrigated with one of the five different irrigating agent respectively. The amount of smear layer and erosion on the surface of the root canal walls were examined with scanning electron microscope., Results: The whole surfaces of root canals in group A and C were covered by smear layer. The surfaces of root canals in the coronal and middle thirds of samples in group B, D, E were free of smear layer (P>0.05) and the apical third of canals in the group D were significantly cleaner (P<0.05) than that in group B and E. The coronal and middle sections were significantly eroded in group B compared with group D and E (P<0.05). The degree of erosion in the apical section was not statistically different among group B, D and E (P>0.05)., Conclusion: Biopure MTAD and 1.3% NaClO are effective irrigating agents for removal of the smear layer on the surface of root canal. There is no significant change in the structure of the surface of root canal.

Purpose: To evaluate whether BioPure MTAD could affect the apical sealing ability of AH Plus sealer in vitro., Methods: Forty-four extracted single-rooted human teeth were randomly divided into six groups. The canals were treated with distilled water,1.3%NaClO,5.25 %NaClO+17%EDTA,1.3%NaClO+BioPure MTAD and 1.3%NaClO+3%EDTA endodontic irrigants, respectively. After obturated, the teeth were made transparent and permitted dye penetration,then measured by using a stereoscopic microscope. SPSS 12.0 software package was used for One-way ANOVA., Results: The microleakages lengths of obturated root canals in group C,D,E,F were (1.81+/-0.73)mm, (0.53+/-0.49)mm, (0.40+/-0.44)mm and (0.39+/-0.55)mm, respectively.Group C had significantly more leakage than group D,E,F(P<0.05), but there were no significant differences among group D,E,F(P>0.05)., Conclusion: Compared with other endodontic irrigants which usually used in clinic, BioPure MTAD doesn't adversely affect the apical sealing ability of AH plus sealer.