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1. [Room temperature phosphorimetric determination of practolol with dansyl chloride labeling].

Author

Tong A, Wu Y, and Li L

Subjects
Sodium Dodecyl Sulfate chemistry, Sulfites chemistry, Dansyl Compounds chemistry, Luminescent Measurements methods, Practolol chemistry, Temperature
Abstract

Dansyl Chloride (DNS-Cl) was used as a phosphorescent labeling agent for the determination of practolol. The derivative was formed by refluxing practolol-ethyl acetate solution with the solid DNS-Cl in the presence of potassium carbonate anhydride. Room-temperature phosphorescent (RTP) properties of the derivative were studied by a micelle-stabilized room-temperature phosphorescence (MS-RTP) method. Phosphorescence lifetimes of practolol-DNS and the DNS-Cl were found to be 0.791 and 0.408ms, respectively. By applying the derivative MS-RTP technique, practolol could be quantitatively determined without previous separation process. Detection limit of practolol with the proposed method was 0.19 microg/mL.

Published
1998

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