Search

Copyright of Journal of Zhejiang University (Science Edition) is the property of Journal of Zhejiang University (Science Edition) Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

To study the function of phytoene synthase gene in carotenoid synthesis of Dimocarpus longan Lour.(D. longan), a DlPSY gene identified from D. longan RNA-seq data was screened and analyzed in this research by bioinformatics methods including the primary structure of protein, physicochemical properties, signal peptide, transmembrane structure, subcellular localization, hydrophilicity, protein secondary structure and tertiary structure, coiled coil, protein binding site, phylogenetic tree, as well as protein-protein interaction. In addition, the real-time quantitative PCR(RT-qPCR)was applied in this research to analyze the expression pattern of DlPSY gene in root and leaf tissues. The bioinformatics software used in this research included NCBI BLAST, DNAMAN, NCBI CD search, Protparam, SignalP 4.1 Server, TMHMM Server, SOSUI, PSORT, ProtScale, SOPMA, COILS, SWISS-MODEL, MolProbity, 3DLigandSite, and STRING. The bioinformatics analysis results were as follows: The length of the DlPSY gene was 1 260 bp, coding for 420 amino acids; the DlPSY protein was predicted to have ‘Isoprenoid Biosyn C1' superfamily structure, contained a signal peptide, did not contain the transmembrane structure, and was a soluble hydrophilic and secretory protein which was predicted to be located outside the membrane; the DlPSY protein secondary structure was predicted to be mainly composed of α-helix and random coil; the DlPSY protein was predicted to have a coiled coil. The Ramachandran evaluation results showed that the tertiary structure model constructed by SWISS-MODEL was reliable, and its ligand binding sites were 344Phe and 347Lys, respectively. In addition, the expression pattern of the DlPSY gene was analyzed by RT-qPCR, the results of which showed that the DlPSY gene was expressed in roots and leaves of D. longan but with the different expression levels. The expression level of DlPSY gene was higher in leaves than that in roots. The results of this study will enrich our knowledge on PSY gene family and also lay a foundation for further improving the carotenoid content in D. longan by genetic methods. [ABSTRACT FROM AUTHOR]

Copyright of Journal of South China Agricultural University is the property of Gai Kan Bian Wei Hui and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Henan Agricultural Sciences is the property of Editorial Board of Journal of Henan Agricultural Sciences and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

[Objective] Neutral invertase was a key enzyme of fruit sucrose metabolism, and cloning neutral invertase gene sequences had a significance for sugar metabolism. [Method]Taken Dimocarpus longan ‘Shixia’ cultivar as tested materials, the whole lengths of its three neutral invertase gene cDNA were cloned and sequenced by RT-PCR and RACE. [Result] Three neutral invertase gene cDNAs named as DlNI-1, DlNI-2 and DlNI-3 , the NCBI numbers were KP769773 , KP769774 and KP769775 , of which the DlNI-1 gene consisted of 2090 bp encoding a polypeptide of 589 amino acids, which had the highest homology with the Manihot esculenta crantz (82 %) through the NCBI blastx; The DlNI-2 gene consists of 2558 bp encoding a polypeptide of 709 amino acids, which had the highest homology with the Citrus Clementina (80 %) through the NCBI blastx; The DlNI-3 gene consists of 2444 bp encoding a polypeptide of 805 amino acids, which had the highest homology with the Citrus clementine (80 %) through the NCBI blastx. Amino acids of three longan neutral invertase gene sequence had 12 highly conservative domain structure and two catalytic residues, it was concluded that the three neutral invertase protein belonged to a class and located in organelles. The expression level of three neutral invertase genes had bigger difference in different tissues, the DlNI-1 and DlNI-2 had the highest expression level in leaves, but the DlNI-3 had the highest expression in pericarp. [Conclusion] Three longan neutral invertase gene were cloned, which would provide references for the investigation of the relation between structure and function of neutral invertase gene. [ABSTRACT FROM AUTHOR]

Objective To optimize the extraction process of proanthocyanidin from longan seed. Methods Proanthocyanidin was extracted from longan seed by adopting the ultrasonic extraction method. The influence of ethanol concentration, solid-liquid ratio, extraction time, extraction temperature, extraction times on the proanthocyanidin extraction rate was investigated by using the single factor experiments and orthogonal experiments. Results The established optimal extraction conditions were 60% ethanol, extraction temperature of 70 ℃, extraction time of 40 min, solid-liquid ratio of 1∶10 and extracting twice. Under these conditions, the extraction rate of proanthocyanidin from longan seeds was 0.652%. Conclusion This study provides the certain theoretical basis for researching the effective extraction and utilization of proanthocyanidins from longan seed. [ABSTRACT FROM AUTHOR]

In order to set up a technical standard for planting Amomum villosum in wood forest in the future, we analyzed the relationship between the ecological factors and the yield of A. villosum planted in five Dimocarpus imocarpus longan wood forests and five miscellaneous wood forests in Yangchun city, to find out the dominant factors that affect the yield of A. villosum. The results showed that agricultural measures of fertilization, artificial irrigation and removing the old plants were positively correlated with the yield of A. villosum, the pesticide spraying and soil pH value were negatively correlated with the yield of A. villosum. But the effects of ecological factors on the yield were not significantly. High yield regions are generally located in the ravine, two sides of mountain stream and other places where water is more adequate. The slope of cultivated field with high yield is generally less than 30°, lighting and ventilation are more appropriate; soil type is generally sandy or loam, shade density is generally about 50%, and pollinators are many in quantity and variety. And we found that there was a large difference in mineral nutrient contents of soils among ten plantations. Results indicate that the yield of A. villosum is determined by the combination of each ecological factor. Suitable light intensity, moisture, ventilation and reasonable fertilization are conductive to increase the yield of A. villosum, but the use of pesticides and soil alkalization hinder the increase of A. villosum production. Too high shade density and the abuse of pesticides may be the main reason for limiting the yield of A. villosum planted in D. longan wood forests. This study has obtained key techniques of the ecological stereoscopic cultivation mode of A. villosum-D. longan, which lays a theoretical foundation for the guidance of farmers in planting A. villosum in the D. Longan forest in the future., Competing Interests: The authors of this article and the planning committee members and staff have no relevant financial relationships with commercial interests to disclose., (Copyright© by the Chinese Pharmaceutical Association.)