1. [Application of throat swab nested PCR in the diagnosis of congenital human cytomegalovirus infection in neonates].
- Author
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Wang XT, Dong ZB, Luo LM, and Deng M
- Subjects
- Fetal Blood virology, Humans, Infant, Newborn, Phosphoproteins blood, Viral Envelope Proteins genetics, Viral Matrix Proteins blood, Cytomegalovirus Infections congenital, Cytomegalovirus Infections diagnosis, Polymerase Chain Reaction methods
- Abstract
Objective: To evaluate the clinical application value of throat swab nested PCR for detecting active congenital human cytomegalovirus (HCMV) infection in neonates., Methods: The throat swabs and umbilical cord blood specimens from 51 neonates were collected for nested PCR assay for HCMV glycoprotein B (gB) gene. Moreover, 18 of them were subjected to a pp65 antigen test., Results: The sensitivity and specificity of throat swab nested PCR for HCMV gB gene were 67% and 75%, respectively, and the positive and negative predictive values were 57% and 82%, respectively., Conclusions: Throat swab nested PCR assay for HCMV gB gene is non-invasive, rapid, and highly sensitive for HCMV detection and holds promise as an excellent screening technology for detecting active congenital HCMV infection in neonates.
- Published
- 2013