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BACKGROUND: Skin-derived precursor cells play a regenerative role in wound healing, which can restore the physiological and aesthetic features of the skin by mitosis, proliferation and migration to the injury region. OBJECTIVE: To observe the structure of the mouse tail and the healing of the broken end, and then to investigate the correlation of these characteristics with the repair of skin trauma at the tail. METHODS: The tails of 2-day-old Kunming mice were cut off using ophthalmic microsurgical scissors to establish the mouse model of hair follicle regeneration in the tail. Regions in which the cells proliferated actively were determined. Wound healing samples were regularly taken, and embedded using OCT. Wound healing of the broken end was routinely observed by pathological staining. Cell proliferation was observed by immunofluorescence. Expression of AP-1 in the healing site was identified by immunofluorescent staining. RESULTS AND CONCLUSION: The structure of the mouse tail was complex, with rich blood supply. Good healing was observed in the broken end of the mouse tail. The cells in the hair follicles and dermal papillary layer showed active proliferation and mitosis, and thereby participated in wound repair and regeneration. Fluorescence staining results found that the positive expression of AP-1 was mainly concentrated in the epidermis and dermal papilla. These findings indicate that the epidermis and dermal papilla possess a strong ability to regenerate. [ABSTRACT FROM AUTHOR]

Objective: To explore a simple and effective method to track the proliferation and differentiation of hair follicle stem cells, and to lay a foundation for the study of the mechanism of proliferation and differentiation of hair follicle stem cells in the later stage. Vibrissa follicle outer root sheath of KM mouse was collected under sterile conditions 5% FCS+MEM was used as basic culture liquid with 4 different concentrations of EdU (5-Ethynyl-2'-deoxyuridine) as cells in the marker to the outer root sheath of the hair follicle regeneration culture and frozen section, after H.E. staining, the regenerative cells labeled with EdU were detected using KeyFluor488. Results: According to the results of H.E staining and the EdU labeling and staining, hair follicle outer root sheath regeneration morphological is worse when the cell marker concentration is high, and the marker effect of hair follicle outer root sheath cell proliferation is the best when the EdU concentration of 20 μmol/L. Conclusion: EdU cell markers of hair follicle outer root sheath stem cell proliferation activity has certain side effects affect on cell's normal physiological function, the concentration of 20 μmol/L EdU can be effectively labeled by the hair follicle stemcell proliferation, and on hair follicle regeneration structure effect is small. [ABSTRACT FROM AUTHOR]

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