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Objective: To explore the cause of 94 cases of asphyxia neonatorum, and find out affirmative foundation for preventing asphyxia neonatorum. Methods: Take retrospective analysis on 94 children of asphyxia neonatorum in our hospital during January, 2011 to January, 2013. Results: The occurrence rate of asphyxia neonatorum can be attributed mainly to premature delivery(27.66%), abnormal fetal position(17.02%) and abnormal amniotic fluid (14.89%). There was a significant difference in the occurrence rate of asphyxia neonatorum between natural delivery and breech delivery (χ²=72.157, P<0.001); There was a significant difference in the occurrence rate of asphyxia neonatorum between natural delivery and fetus aspiration (χ²=28.172, P<0.001); There was not significant difference in the occurrence rate of asphyxia neonatorum between natural delivery and cesarean delivery (χ²=5.169, P=0.053). Conclusions; The main cause of neonatal asphyxia were premature birth, fetal abnormalities, abnormal amniotic fluid, breech births and fetal absorption capacity. We should pay more attention to antenatal care and prenatal examination so as to effectively reduce the occurrence of asphyxia neonatorum and guarantee the health of mothers and infants. [ABSTRACT FROM AUTHOR]