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BACKGROUND: Nobiletin has been found to improve lipopolysaccharide-induced abnormal activation of microglia, excessive release of inflammatory factors and redox imbalance. However, the specific mechanism is not fully understood. OBJECTIVE: To investigate the molecular mechanism by which nobiletin can inhibit lipopolysaccharide-induced inflammation in BV2 microglia. METHODS: Passage 3 BV2 microglia were divided into three groups: control group was cultured for 24 hours (without any treatment). Lipopolysaccharide group was treated with 10 μg/mL lipopolysaccharide for 24 hours. Lipopolysaccharide + nobiletin group was treated with 20 μmol/L nobiletin for 6 hours and then 10 μg/mL lipopolysaccharide for 24 hours. After the processing, cell proliferation was detected by CCK-8 assay. The level of intracellular reactive oxygen species was detected by fluorescent probe. The mRNA expression levels of nuclear factor κB p65, tumor necrosis factor α, and interleukin-1β were detected by qRT-PCR. The protein expression levels of nuclear factor κB p65, p-nuclear factor κB p65, tumor necrosis factor α, and interleukin-1β were detected by western blot assay. RESULTS AND CONCLUSION: Compared with the control group, the proliferation activity of lipopolysaccharide group was decreased (P < 0.001). Compared with the lipopolysaccharide group, the cell proliferation activity of lipopolysaccharide + nobiletin group was increased (P < 0.001). Compared with the control group, the level of intracellular reactive oxygen species was increased in the lipopolysaccharide group (P < 0.001). Compared with the lipopolysaccharide group, the level of intracellular reactive oxygen species was decreased in the lipopolysaccharide + nobiletin group (P < 0.01). Compared with the control group, the mRNA expression levels of tumor necrosis factor α and interleukin-1β were increased in the lipopolysaccharide group (P < 0.001, P < 0.01). Compared with the lipopolysaccharide group, mRNA expression levels of tumor necrosis factor α and interleukin-1β were decreased in the lipopolysaccharide + nobiletin group (P < 0.01, P < 0.05). Compared with the control group, the protein expression levels of p-nuclear factor κB p65, tumor necrosis factor α, and interleukin1β in were increased the lipopolysaccharide group (P < 0.001). Compared with the lipopolysaccharide group, the expression of p-nuclear factor κB p65, tumor necrosis factor α, and interleukin-1β was decreased in the lipopolysaccharide + nobiletin group (P < 0.001). These findings suggest that nobiletin attenuates lipopolysaccharide-induced inflammatory response in BV2 microglia by suppressing nuclear factor-κB signaling pathway. [ABSTRACT FROM AUTHOR]

Objective: To investigate the significance of dynamic quantitative detection of (cytomegalovirus) CMV antibodies in assessing the risk of fetal CMV infection. Methods: A total of 171 pregnant women with positive/weakly positive CMV IgM antibody ELISA screening results from Huizhou Central People's Hospital from August 2019 to March 2022 were selected as the study objects. IgM and IgG levels were measured by electrochemical luminescence (ECLIA), and re-examined 2 to 4 weeks later;at the same time, CMV nucleic acid was detected by real-time PCR fluorescent probe, and the fetal outcome was followed up. Results: In 171 cases, 135 CMV IgM antibody ELISA (+)/ECLIA (-) specimens were positive for IgG antibodies and had stable levels after 2~4 weeks, one case was diagnosed with severe β thalassemia at 19+5 weeks, and the remaining fetuses were in good condition. There were 36 cases of IgM antibody ELISA (+)/ECLIA (+) specimens, one was negative for IgG antibody; 35 cases had positive IgM antibody and stable IgG antibody level after 2~4 weeks, the fetus was in good condition; one case had IgM antibody level decreased and IgG antibody level increased significantly, urinary CMV DNA level gradually decreased, and was diagnosed as disseminated congenital CMV infection. Conclusion: The false positive rate of CMV IgM antibody detected by ELISA method is significantly higher than that of ECLIA method, and the dynamic quantitative detection can effectively eliminate interference. Maternal women with significant changes in CMV IgM, IgG antibodies and urinary DNA levels are at high risk of intrauterine infection and had a high probability of fetal congenital CMV infection. [ABSTRACT FROM AUTHOR]

On-site detection of heavy metals in groundwater is important to quickly evaluate pollution. Laser induced fluorescence (LIF) utilizes specific fluorescent probes to generate/quench fluorescence in the presence of heavy metals, thereby achieving heavy metals detection, which can quickly identify heavy metals and nondestructively obtain their valence states. This work summarizes the principle and equipment of, and its application for, in situ detection of typical heavy metals in groundwater. At present, LIF probes used for heavy metal detection include organic fluorescent probes mainly composed of small molecule probes, macromolecules, and AIE probes, as well as nanomaterial probes represented by gold nanoclusters, QDs, and MOFs. These synthesized probes and the corresponding constructed sensors indicate that LIF holds significant advantages in heavy metal detection in groundwater. Although the achievements in the development of LIF equipment for heavy metal detection in groundwater are not as rich as those in sensing methods, LIF equipment for several heavy metals have been developed, demonstrating good application prospects. Future research will focus on identifying the controlling factors of heavy metals in groundwater and developing anti-interference techniques, synthesizing novel fluorescent probes for LIF sensor, integrating sensing components into LIF equipment, and standardizing the LIF detection process. [ABSTRACT FROM AUTHOR]

Objective: To comp compare the value of acid-fast staining, PCR-reverse dot blot hybridization and PCR-fluorescent probe in the diagnosis of Mycobacterium tuberculosis infection. Methods: Samples from the Eighth Medical Center of the PLA General Hospital from January 2024 to April 2024 were selected, including suspected pulmonary tuberculosis and suspected extrapulmonary tuberculosis. Eighty samples were prepared into paraffin samples and tested for Mycobacterium tuberculosis by acid-fast staining, PCR-reverse dot blot hybridization and PCR-fluorescent probe. Results: The detection rates of PCR-reverse dot blot hybridization were 63.6%, PCR-fluorescent probe 59.1%, and acid- fast staining 25.0%. The detection rates of PCR-reverse dot blot hybridization and PCR-fluorescent probe were higher than those of acid-fast staining, and P<0.05. The positive detection rate of PCR-reverse dot blot hybridization method and PCR-fluorescent probe method was basically the same. The acid-fast staining method has the characteristics of low detection cost and short experimental time, but it cannot clearly determine whether it is Mycobacterium tuberculosis infection based on morphology alone. The detection cost of PCR-reverse dot blot hybridization method was higher than that of acid-fast staining method, and the experimental time was longer than that of acid-fast staining method and PCR- fluorescent probe method. The PCR-fluorescent probe method is moderately expensive, but the detection time is shorter than that of PCR-reverse dot blot hybridization. Conclusions: The PCR- fluorescent probe method has a high detection rate, fast and simple operation, and moderate cost, which has good clinical application value for hospitals with a large number of samples. [ABSTRACT FROM AUTHOR]

The novel Fe doped carbon dots(Fe-CDs)with good photoluminescent properties were rapidly synthesized by microwave-assisted method with ferric citrate and polyethyleneimine as raw materials. The results show that the Fe-CDs have good stability under the conditions of broad pH range from 3 to 9, high ion strengths and long storage time. The prepared Fe-CDs can easily penetrate and accumulate into the cell, which can be used as effective probe for biological imaging of A549 cells. The Fe-CDs display distinct peroxidase-like (POD) activities, which is evidenced by the catalyzed oxidation of 3, 3', 5, 5'-tetramethylbenzidine(TMB)into blue oxidized products in the presence of H2O2 . A colorimetric method for the determination of H2O2 established based on the results. Under optimal reaction conditions, the colorimetric method for H2O2 detection is constructed with good linear relationship from 0 to100. 00 μmol/L and detection limit down to 0. 13 p. mol/L. The highly selective and ultrasensitive method is successfully applied to detect H2O2 inside A549 tumor cells with good recoveries of 98. 4％-102. 8％, which can verify the proposed method. [ABSTRACT FROM AUTHOR]

Copyright of Shipin Kexue/ Food Science is the property of Food Science Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Chinese Journal of Inorganic Analytical Chemistry / Zhongguo Wuji Fenxi Huaxue is the property of Beijing Research Institute of Mining & Metallurgy Technology Group and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

The traditional TVB-N method for evaluation of freshness has defects such as complicate process, delay result and other defects. Therefore, it is extremely significant to develop a rapid, non-destructive and real-time approach for detecting the freshness of salmon. In this paper, a benzopyran derivative HDX was constructed through condensation reaction. HDX could sensitively recognize 10 amines, moreover, within the EtOH/H2O (volume ratio 1:9) system, obvious color and fluorescence changes occurred within 5 s. The detection limit of HDX for spermine was 0.75 µmol/L. By utilizing HDX, fluorescence imaging of spermine and putrescine could be achieved in living cells. The sensing label HDXIL was prepared by loading HDX onto filter paper, and HDXIL could respond for fish freshness by colorimetric and fluorescence dual channels. This sensor label HDXIL showed significant recognition effect on different grades of salmon meat (distinguished by three colors). Its evaluation results were highly accurate and trustworthy, and it was suitable for real-time and on-site monitoring of the freshness of salmon meat. [ABSTRACT FROM AUTHOR]

Copyright of Science & Technology of Food Industry is the property of Science & Technology of Food Industry Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Biothiols play an important role in cell growth and differentiation, metabolism and redox balance. In this paper, a new “off-on” fluorescent probe, HF-DBS, was designed and synthesized from the starting material 4-dimethylaminobenzaldehyde. The probe can be selectively hydrolyzed by biothiols to produce the 3-hydroxyflavone fluorophore (HF) with strong fluorescence emission, which has the property of excited state intramolecular proton transfer (ESIPT) and therefore has a large Stokes shift. In addition, the probe has high selectivity, high sensitivity, good biocompatibility and anti-interference ability, it can be used under physiological conditions. Thus, this probe has been successfully applied to intracellular biothiols detection and live cell imaging. In addition, the probe can be made into test paper for rapid cysteine detection. [ABSTRACT FROM AUTHOR]

The detection of pH and water is critical in the life sciences and chemical industries. The yellow fluorescent carbon dots (CDs) synthesized by solvothermal method using neutral red (NR) as carbon source, ethylenediamine (EDA) as nitrogen source and ethanol as solvent was studied. It was found that the carbon dots (NR-EDA-CDs) could produce double emission peaks when the excitation wavelength was in the range of 310~370 nm. Therefore, a method for the detection of pH and water with a double ratio fluorescence probe based on a single luminant was established. The carbon dots were characterized by high-resolution transmission electron microscopy, X-ray diffraction, Raman spectroscopy, infrared spectroscopy and X-ray photoelectron spectroscopy. The results showed that the carbon dots are spheroidal in shape, with an average particle size of 3. 6 nm and obvious graphite structure. It was found that the carbon dots has good stability, photobleaching resistance and salt tolerance at the excitation wavelength of 365 nm. In phosphate buffer solution, pH in the range of 4. 15~8. 55 has a good linear relationship with the fluorescence intensity ratio of 581 and 449 nm (F581/ F449), and its coefficient (R²) is 0. 986. The pH ratio fluorescent probe has the characteristics of wide linear range and good reversibility. At the same time, it was found that the content of water in DMSO is linear with F485/ F585 in the range of 0. 6%~50% (V (H2O) / V (DMSO)), R² is 0. 996, and the detection limit is 0. 18% (V (H2O)/ V (DMSO)) . The double ratio fluorescent probe provides a new method for the detection of pH and water. [ABSTRACT FROM AUTHOR]

This study aimed to obtain a lentinan-specific fluorescent probe for the in situ visualization of lentinan. A carbohydrate-binding module with potential lentinan-binding capacity was discovered in a β-1,3-D-glucanase by using the bioinformatics techniques, and further recombinantly expressed. The binding specificity of the expressed protein, which was named as LBM was determined by the microtiter plate assays. The results indicated that LBM displayed a desired specificity for lentinan. The protein exhibited positive binding signals to lentinan, while could not bind to several examined polysaccharides including barley β-glucan, curdlan, or konjac glucomannan. Furthermore, the first lentinan-specific fluorescent probe was successfully constructed by fusing LBM with a green fluorescent protein EmGFP, based on which the in situ visualization of lentinan in Lentinula edodes was realized. It was shown that lentinan was not only presented in the mycelial cell wall, but also amorphously distributed in the interstitial space and inside the cell. The construction of the EmGFP-LBM provided a promising tool for the in situ visualization of lentinan. [ABSTRACT FROM AUTHOR]

Copyright of Journal of Chinese Institute of Food Science & Technology / Zhongguo Shipin Xuebao is the property of Journal of Chinese Institute of Food Science & Technology Periodical Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Tiangong University is the property of Journal of Tianjin Polytechnic University and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Chongqing University of Technology (Natural Science) is the property of Chongqing University of Technology and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

AIM: To investigate the mechanism by which wogonin (WOG) induces ferroptosis in collagen-induced arthritis rat fibroblast-like synoviocytes (rat CIA-FLS cells) through the nuclear factor E2-related factor 2 (NRF2)/heme oxygenase-1 (HO-1) signaling pathway. METHODS: Rat CIA-FLS cells were divided into: control group, low, medium, and high dose of (25, 50 and 100 μmol/L) WOG group, ferroptosis inhibitor (LIP-1) group, LIP-1 + high dose WOG group, HO-1 agonist cobalt protoporphyrin (COPP) group, and COPP + high dose WOG group. CCK-8 assay was used for cell viability. Crystal violet staining was used for for cell morphology. The levels of oxidative stress markers glutathione (GSH), malondialdehyde (MDA), and superoxide dismutase (SOD) were measured. DCFH-DA fluorescent probe was used to detect the intracellular reactive oxygen species (ROS) content as well as Western blot to detect the protein expression levels of Kelch-like ECH-associated protein 1 (KEAP-1), NRF2 and HO-1. RESULTS: Compared with the normal control group, administration of WOG treatment resulted in a significant decrease in CIA-FLS cell viability (P< 0. 01, a significant increase in the level of oxidative stress (P<0. 01), a significant increase in the content of ROS (P< 0. 01), a significant decrease in the level of expression of NRF2 and HO-1 proteins (P<0. 01), and a significant increase in the level of KEAP-1 (P<0. 01) in the rat. Compared with the WOG group, the LIP-1-treated group showed a significant increase in cell viability (P<0. 01, a significant decrease in the level of oxidative stress (P<0. 01), and a significant decrease in the content of ROS (P<0. 01). Compared with the WOG group, the addition of COPP resulted in a significant increase in the protein expression levels of NRF2 and HO-1 (P<0. 01) and a significant decrease in KEAP-1 levels (P< 0.01). CONCLUSION: WOG can induce ferroptosis in rat CIA-FLS cells by promoting oxidative stress through the NRF2/HO-1 signaling pathway. [ABSTRACT FROM AUTHOR]

A near-infrared region (NIR) ratiometric fluorescent probe Cy-P based on hemicyanine was rationally designed, prepared and utilized for the detection of exogenous and endogenous peroxynitrite (ONOO-) in living cells. The research results show that Cy-P itself displays NIR emission owing to the large π-conjugated system. With the addition of the strong oxidizer ONOO-, the π-conjugated system of Cy-P is destroyed, and a new substance with blue fluorescence is formed, leading to 268 nm blueshift of fluorescent emission. The linear detection range of the probe Cy-P to ONOO- is 0-15 μmol/L, and the detection limit is 13 nmol/L. The probe exhibits superior selectivity for ONOO- over various potentially interfering biological analytes. Due to the low cytotoxicity, the prepared probe is suitable for imaging detection of exogenous and endogenous ONOO- in living cells. [ABSTRACT FROM AUTHOR]

BACKGROUND: Myocardial cell hypoxic injury is closely associated with oxidative stress and ferroptosis.Previous studies have shown that aloin has various effects such as antioxidant, anti-inflammatory, and anti-tumor activities. OBJECTIVE: To investigate the effects of aloin on oxidative stress and ferroptosis in hypoxia-induced H9C2 cells. METHODS: A hypoxia model was established using H9C2 myocardial cells.Firstly, cell viability was determined to confirm the lack of cytotoxicity of aloin and to determine its optimal therapeutic concentration.Subsequently, the effects of aloin on hypoxia-induced lactate dehydrogenase release, reactive oxygen species production, and mitochondrial oxidative stress in H9C2 cells were evaluated using assay kits, dihydroethidium fluorescent probes, and MitoSOX?Red fluorescent probes, respectively.To verify the effect of aloin on ferroptosis, intracellular Fe2+content and lipid peroxidation level were detected using fluorescence staining and flow cytometry, respectively.Then, the expression levels of ferroptosis regulatory factors glutathione peroxidase 4, acyl-CoAsynthetase long-chain family member 4, and nuclear factor E2-related factor 2 were detected using western blot assay and real-time fluorescence quantitative PCR techniques.Finally, the role of ferroptosis in aloin-mediated myocardial protection was further confirmed by using the ferroptosis inducer Erastin. RESULTS AND CONCLUSION: (1) Compared with the control group, the viability of H9C2 cells in the hypoxia group was significantly decreased, lactatedehydrogenase release, reactive oxygen species level, mitochondrial oxidative stress degree, Fe2+content, and lipid peroxidation degree were significantlyincreased, while glutathione peroxidase 4 and nuclear factor E2-related factor 2 mRNA and protein expression levels were significantly decreased, and acyl-CoA synthetase long-chain family member 4 mRNA and protein expression were significantly increased (all P＜0.05). (2) Compared with the hypoxia group, both low and high doses of aloin reversed the changes in above indicators (all P＜0.05). (3) Compared with the hypoxia+aloin group, the hypoxia+aloin+Erastin groupshowed a significant decrease in H9C2 cell viability and a significant increase in lactate dehydrogenase release (both P＜0.01). The results indicate that aloin has a protective effect on hypoxia-treated H9C2 cells in a dose-dependent manner, mainly achieved by inhibiting oxidative stress and ferroptosis. [ABSTRACT FROM AUTHOR]

In situ fluorescence imaging technology offers an efficacious method for garnering biochemical information at cellular and organismal scales. Prolonged in situ labeling techniques, using solid-state fluorescent probes, are particularly suited for precise imaging and surgical navigation during tumor resections. However, the inherent challenges, including extended research cycles, high costs, and substantial resource utilization, often preclude their inclusion in undergraduate teaching. To surmount these obstacles, we transformed the latest research findings into a "virtual simulation experiment of tumor in situ fluorescent labeling technology design and exploration" utilizing the U3D three-dimensional virtual reality technology platform. Through an interactive 37-step operation and exploratory teaching approach centred on crucial scientific issues, we aim to hone students' practical skills, foster scientific thinking and innovative awareness, actively guide the development of their scientific literacy, and instil values pertinent to scientific research. [ABSTRACT FROM AUTHOR]

A metal-organic framework complex {[Zn2 (OH) ( TZI)·2H2O]·6DMF} n (1) ( TZI = 5-tetrazolylisophthalic acid) was successfully isolated under solvothermal conditions. The structure of the complex was characterized by single crystal X-ray diffraction, elemental analysis, infrared spectroscopy, thermogravimetric analysis, powder X-ray diffraction. Single crystal X-ray diffraction study reveals that 1 displays a 3D structure with channels, where exist uncoordinated carboxylate groups and coordinated water molecules. Subsequently, through post-synthesis method, a Eu(III)@ 1 with red fluorescence emission was obtained by introducing Eu(III) into framework of 1. Under 275 nm wavelength excitation, Eu(III)@1 possesses two groups of fluorescence emission. In the first group, the wide peak at 418 nm is the fluorescence emission of 1, which origins from the absorption and emission of the organic ligand. While, the second group of five emission peaks is the red characteristic emission peak of Eu(III) ion, with main peak of 619 nm. The results of anti-interference experiments of other components in urine show that Eu(III)@1 fluorescent probe is very sensitive to the biomarker 1-naphthol, and can lead to red fluorescence quenching (619 nm), accompanies by cyan fluorescence enhancement (465 nm), and has high selectivity for 1-naphthol. Fluorescence titration results show that the detection limits of Eu(III)@1 for 1-naphthol based on fluorescence emission peaks at 465 and 619 nm are 6. 95 μmol / L and 1. 75 mmol / L, respectively. [ABSTRACT FROM AUTHOR]

Copyright of Food Research & Development is the property of Food Research & Development Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

This study presents a research-oriented comprehensive chemistry experiment project. Specifically, a fluorometric sensor based on a nickel nanocluster (NiNC)-europium complex (denoted as NiNCs-Eu3+) was developed for the detection of tetracyclines (TCs). This experiment includes the preparation and characterization of NiNCs-Eu3+, performance analysis of the response to TCs, construction of the paper-based sensor, and investigation of its visual identification. Overall, this "visible" integrated chemistry experiment is expected to promote the development of comprehensive experimental skills and research capabilities of students because it involves a strongly comprehensive process and simple operation and exhibits high feasibility. [ABSTRACT FROM AUTHOR]

Copyright of Journal of Food Safety & Quality is the property of Journal of Food Safety & Quality Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

The main clinical problems in treatment of oral cancer are tumor recurrence and metastasis after surgery. To improve the efficiency of resection and the prognosis of patients is a key to curing oral cancer. Fluorescence imaging, as an adjuvant in the diagnosis and treatment of oral cancer with no invasion and radiation, can provide real-time and ultrahigh-resolution graphics for preoperative tumor diagnosis, intraoperative tumor margin determination and postoperative tumor bed checking, which can help reduce the recurrent rate, enhance the survival rate and improve the life quality of patients. This article reviewed several typical fluorescent probes and discussed their characteristics, limitations and application prospects in clinic or research for oral cancer. [ABSTRACT FROM AUTHOR]

Copyright of Journal of Henan Agricultural Sciences is the property of Editorial Board of Journal of Henan Agricultural Sciences and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

In this study, a method for identifying residual antibiotics in food was developed based on chromium black T and europium ion as colorimetric and fluorescent probes (EBT/Eu3+) coupled with pattern recognition in machine learning. The changes in color and fluorescence of four antibiotics with the addition of EBT/Eu3+ were obtained using smart phone and fluorescence measurements. Assisted by pattern recognition, the four antibiotics in honey were successfully identified. This experiment is highly comprehensive; it integrates multiple knowledge points, such as twochannel probe preparation, color recognition based on smart phone, fluorescence measurements, machine learning, and identification of various antibiotics. [ABSTRACT FROM AUTHOR]

This work is aimed to construct a KYSE-150 cell line containing an inducible expressed ERK-SPARK kinase fluorescent sensor into the AAVS1 locus. The homologous recombination donor plasmid of the inducible ERK-SPARK expression cassette with 750 bp homology arms at both ends was constructed according to the DNA sequence design of the AAVS1 locus and the published base sequences of the ERK-SPAEK fluorescent probe. Secondly, three sgRNAs targeting the AAVS1 locus were designed using an online design tool, and cloned into the pX330 backbone plasmid. Three CRISPR/Cas9 targeting vectors capable of site-specific cutting of the AAVS1 locus were obtained. The homologous recombination donor plasmids and the expressed CRISPR/Cas9 targeting vectors were co-transfected into KYSE-150 cells, after Dox-induced expression for 24 h, puromycin resistance screening and flow cytometry were performed, and cell subpopulations resistant to puromycin and expressing GFP were obtained. Then PCR was used to identify the genotype, and Western blot was used to detect protein expression. Genomic DNA was extracted from the obtained cell population and the genotype was identified by PCR. The results showed that the size of the obtained fragment was consistent with the size of the inducible ERK-SPARK expression cassette. The results of protein expression detection showed that the cell population expressed the ERK-SPARK probe fusion protein. The results of fluorescent probe imaging experiments demonstrated that the cell line had a very small amount of leakage expression under the condition of no Dox-induced expression. After Doxinduced expression, the GFP fluorescent protein was evenly distributed in the cytoplasm without obvious aggregation into spots. After adding EGF to activate ERK kinase, the green fluorescence of GFP that was uniformly distributed in the cytoplasm gathered into high-brightness green droplets. A stable cell line that expressed the SPARK fluorescent probe after induction for detecting ERK kinase activity were successfully obtained, which may solve the problem that the unstable efficiency of the transiently expressed ERK-SPARK probe affected the accuracy of the experimental results, and may lay the preliminary research foundation for subsequent establishment of screening platform of a SPARK-based high-throughput protein kinase inhibitors. [ABSTRACT FROM AUTHOR]

The ability to realize logic calculations and applications of molecules from the nanoscale is an important part of miniaturization of electronic microprocessors. Herein, a novel fluorescent probe complex （BSA@SALPHENH2 ） is prepared by the interaction of bis-（salicyladehyde）-1,3-propylenedii-mine （SALPHENH2 ） with bovine serum albumin（BSA). The interaction of BSA with bis-（salicyladehyde）-1,3-propylenediimine is characterized by fluorescence titration experiment, synchronous fluorescence spectroscopy, site marker fluorescent probes and molecular docking. It is found that the conformation of BSA changes slightly in presence of bis-（salicyladehyde） -1, 3-propylenediimine, the quenching type is static quenching, and there is van der Waals forces and hydrogen bonding in Subdomain ⅢA （Site Ⅱ） of BSA. Additionally, the experiment results show that the complex has two fluorescence emission bands under the excitation of 280 nm due to hybrid fluorescence resonance energy transfer and electron exchange energy transfer between BSA and Zn2+ -bis-（salicyladehyde） -1, 3-propylenediimine Schiff base. Then, Modulating the system using different ions as the input signal to design a half subtractor and a binary molecular logic circuit containing one OR gate, one NAND gate, two NOT gates and three AND gates. Simultaneously, this combination provides a good reference model in exploring Dexter energy transfer in fluorescent experiments. [ABSTRACT FROM AUTHOR]

Copyright of Shipin Kexue/ Food Science is the property of Food Science Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Food Safety & Quality is the property of Journal of Food Safety & Quality Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Herein, we apply a state-of-the-art fluorescence detection technology from scientific research to teaching. By optimizing the molecular structure and changing the properties of fluorescent probes, we overcome the shortcomings of BODIPY (fluoroboron dipyrrole) fluorescent dyes, such as short emission wavelengths and poor hydrophilicity. A novel hypochlorite fluorescent probe with a fast response, high selectivity, and good sensitivity was synthesized through the design and improvement of the structure of the BODIPY fluorescent dye. The obtained structure of the fluorescent probe was characterized, and the fluorescence properties of the probe were measured. Through the study of the relationship between the probe structure and fluorescence properties, students will have a better understanding of the mechanism of fluorescent probe recognition and the basic principles of fluorescence and luminescence. The experimental part includes organic synthesis and purification, instrumental characterization of the products, and evaluation of the obtained results. This experiment is comprehensive and simple to perform. The methodical experimental procedure is beneficial for cultivating scientific thinking and the students' ability to connect theory with practice. Moreover, this experiment can improve their practical skills, and develop the ability to data process and perform literature research in Chinese and other foreign languages. [ABSTRACT FROM AUTHOR]

Copyright of Journal of Modern Laboratory Medicine is the property of Journal of Modern Laboratory Medicine Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Cu(I)-catalyzed azide–alkyne cycloaddition (CuAAC) click chemistry and fluorescent probe are hot research topics of organic chemistry. Introducing it into undergraduate laboratory could help to cultivate creativity of students. However, how to avoid the use of azides is a great challenge to promote CuAAC click chemistry in undergraduate education. Herein, we report a modified CuAAC click reaction experiment for undergraduate laboratory teaching, featuring improved safety and interestingness. 5,7-dimethyltetrazolo[1,5-a]pyrimidine can be tautomerized into azide form in solution. Elegantly using this property could achieve the CuAAC reaction between 5,7- dimethyltetrazolo[1,5-a]pyrimidine and 4-acetylene anisole for the generation of triazole compound 5, avoiding the toxicity and explosive risk from the operation of azide compound. The synthesized compound 5 could selectively recognize zinc ions as a fluorescent probe. It is potentially useful for quantitative analysis of zinc ion concentration. [ABSTRACT FROM AUTHOR]

As an important biologically active molecule, peroxynitrite (ONOO-) plays an important role in cellular signal transduction and homeostatic regulation. Precise and targeted detection of ONOO- in biological systems is crucial for in-depth exploration of its physiological and pathological mechanisms. A "turn-on" fluorescent probe naphthalamide derivative (NA) is designed based on 1, 8-naphthalene imide and ursodeoxycholic acid for specific detection of ONOO- in HepG2 cells. Probe NA is synthesized by three steps, the structures of intermediates and target compounds are confirmed by ¹H NMR and 13C NMR spectroscopy and HRMS. The optical properties of probe NA are explored. The green fluorescence of probe NA in the presence of ONOO- is significantly increased compared to its fluorescence in the absence of ONOO-. The fluorescence intensity and concentrations of ONOO- shows a good linear relationship in the range of 0. 0 to 14. 0 μmol/L, with the detection limit of 0. 27 μmol/L. In addition, probe NA shows excellent selectivity toward ONOO-, good biocompatibility, and the fluorescence intensity is almost independent to the pH of the environment. Finally, probe NA is applied to monitor ONOO- in living cells. The results of cell fluorescence imaging exhibit that probe NA possesses good targeting ability to HepG2 cells, and can detect exogenous and endogenous ONOO in HepG2 cells, which demonstrates the great potential of in the application of ONOO- detection in vivo. [ABSTRACT FROM AUTHOR]

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In this paper, carbon quantum dots was synthesized by one-step hydrothermal method using camphor leaves powder as the precursor, and a fluorescent method for Fe3+ detection was established by using it as the fluorescent probe. The results show that, at the excitation wavelength of 320 nm, the ratio of fluorescence intensity of CQDs solution after adding Fe3+ to CQDs solution without Fe3+ addition F/F0 and Fe3+ concentration shows a good linear relationship in the range of 2.72×10-5～1.00×10-4 mol/L(R²=0.9912), and the limit of detection is 8.16 μmol/L. When it is applied to the determination of iron content in iron supplements, the recovery rates of standard addition is 93.08%～105.05%. The preparation method of the carbon quantum dots is simple and fast, the raw material is environmentally friendly and easy to obtain, the method for Fe3+ detection has good linearity, high sensitivity and good selectivity, and the carbon quantum dots can be used for the analysis of iron content in actual iron supplements. [ABSTRACT FROM AUTHOR]

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Biothiols, such as cysteine(Cys), homocysteine (Hcy) and glutathione (GSH) play a central role in intracellular regulation of redox homeostasis and maintenance of cell functions.In this work, a fluorescent probe based on TTCNPy nanoparticles was been synthesized for the detection of biothiols.This probe had a near-infrared (NIR) fluorescence (λem=727 nm) and a large Stokes shift (260 nm) .It can sensitively detect biothiols in buffer solution.The detection limits of Hcy, Cys and GSH were calculated to be 0.479,0.429,0.480 μmol, respectively.Moreover, this probe had high selectivity and anti-interference ability due to the strong nucleophilic attack ability of the sulfhydryl group in biothiols which can destroy the conjugated structure and subsequently quench the fluorescence of probe.This probe had the advantages of simple synthesis, high sensitivity, high selectivity and low cytotoxicity, and can be used to detect biothiols in solution and living cells. [ABSTRACT FROM AUTHOR]

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