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Objective: To establish a method for the determination of two perfluorinated compounds in urine by liquid chromatography-tandem mass spectrometry. Methods: In November 2022, urine samples were extracted by acidic methanol, purified by WAX solid phase extraction column, and eluted with methanol water, then Waters ACQUITY UPLC BEH C18 column (100 mm×2.1 mm, 1.7 μm) was used with 1.0 mmol/L ammonium acetate solution and methanol as mobile phase. The gradient elution was carried out, the detection was carried out by electrospray negative ion multiple response monitoring (MRM) mode, and the quantitative method was internal standard method. Results: Perfluorooctanoic acid and perfluorooctane sulfonic acid had a good linear relationship in the concentration range of 0.5-50.0 μg/L, and the correlation coefficient was >0.999. The limit of detection was 0.017 μg/L, and the limit of quantitation was 0.005 μg/L. The average recoveries were 96.3% and 101.8%, respectively. Days of precision were 3.5%-6.2% and 3.1%-7.4%, respectively, daytime precision were 4.3%-6.8% and 4.7%-8.1%, respectively. Conclusion: The established method of liquid chromatography-tandem mass spectrometry is high sensitivity and accuracy, and is suitable for the determination of perfluorooctanoic acid and perfluorooctane sulfonic acid in human urine.

Perfluorinated and polyfluoroalkyl substances (PFASs) are a new type of persistent organic pollutants(POPs) that are widespread in the environment and organisms and have many aspects of toxicity. Some experimental and epidemiological findings have shown that PFASs exposure is associated with ovarian cancer in women. In recent years, domestic and foreign reports on the relationship between PFASs and ovarian cancer have been increasing, among which the toxicology, epigenetics and the corresponding carcinogenic mechanism of PFASs exposure have become an important topic of discussion. This paper reviews the latest research progress on the relationship between PFASs and ovarian cancer, and discusses the existing problems and future research directions of PFASs. [ABSTRACT FROM AUTHOR]

Polyfluorinated chemicals (PFCs) are a series of endocrine disruptors (EDCs) generated from industrial production and existing in environment of daily life. PFCs can be accumulated in animal tissues, and perform reproductive toxicity through different mechanisms such as disrupting steroidogenesis, inducing apoptosis of reproductive cells and acting as antagonism of hormone receptors. In male, PFCs were found to be associated with the decreased sperm quality and concentration, and the increased rate of sperm deformation, and to disturb sexual hormones such as testosterone. In female, PFCs increased in blood or follicular fluid were related to the decreased fertility. Moreover, the exposure to PFCs during pregnancy may affect the health and fertility of offspring. The molecular mechanisms of this kind of chemicals have been studied in many animal experiments. In conclusion, we should pay attention to the reproductive toxicity of PFCs. [ABSTRACT FROM AUTHOR]

Objective: To analyze the health hazards of fluorine and its inorganic compounds to workers exposed to fluorine, and to provide technical support for the protection of workers exposed to fluorine and the revision of occupational disease diagnostic standards. Methods: In January 2019, 677 workers exposed to fluorine in a fluorine chemical company in Hunan Province were selected by cluster sampling, and occupational health examination was conducted. The suspected occupational poisoning workers were diagnosed as occupational diseases, which were divided into poisoning group and non-poisoning group. T test and Pearson χ(2) test were used for statistical analysis. Results: A total of 73 occupational chronic fluorosis patients were diagnosed. 93.15% (68/73) of the skeletal lesions were multiple, and the most frequent sites were the upper tibia and fibula. 35.00% (21/60) , 50.00% (18/36) and 58.82% (10/17) of the tibia, fibula, ulna and radius had periosteal changes only on one side. Other abnormal results were mainly dental calculus (60.71%, 411/677) , fatty liver (48.89%, 331/677) , abnormal electrocardiogram (44.17%, 299/677) , decreased bone mass (33.53%, 227/677) and increased ALT (13.15%, 89/677) . Compared with non-poisoning group, the age, length of service exposed to fluoride and fatty liver detection rate of poisoning group were higher, and the differences were statistically significant ( P <0.01) . Conclusion: The changes of long bone caused by inorganic fluoride only occur in one side, so the basic location of occupational health examination should include bilateral long bone to avoid missed diagnosis. It is difficult to diagnose occupational poisoning with single slight periosteal ossification, it is suggested that the standard should be revised to specify the degree.