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Hyperbolic thin-shell cooling towers have complicated vibration modes, and are very sensitive to the effects of group towers and wind-induced vibration. The aero-elastic models of cooling tower in wind tunnel tests are usually designed based on the method of stiffness simulation by continuous material structure. However, the method has some shortages in actual engineering application, so the so-called "equivalent beam-net design method" of aero-elastic model of cooling tower is proposed in this paper. Reduced-scale stiffness algorithm of cooling tower model is formulated firstly, and corresponding modification and simulation methods about aerodynamic effects of Reynolds and Strouhal number are also suggested. An aero-elastic model of 1 : 200 ratio of cooling tower was manufactured using beam-net method, then in Tongji university TJ-3 wind tunnel, dynamic response tests were carried Out. It can be confirmed that vibration modes and aerodynamic parameters, wind-vibration factors and testing feasibility meet the requirement of wind tunnel tests, the proposed method can avoid the short-ages of traditional method. [ABSTRACT FROM AUTHOR]

Aim: To prepare specific single-epitope antibody using immunomagnetic beads coated with multiple antigen peptides(MAP)., Methods: Eight-branch MAP is synthesized by Fmoc SPPS. After immunizing the BALB/c mice with the MAP, polyclonal antiserum was obtained. Then, the immunomagnetic beads (IB) were prepared by coating with the MAP in covalent bond form and were used to purify the specific single-epitope antibody from the antiserum of immunized animals., Results: The titer of antiserum was 1:12,800, which showed the strong immunogenicity of MAP. The best coating concentration of MAP was 100 mg/L and the max coating efficiency was 79%. After purifying specific antibody from polyclonal antiserum using the IB, the obtained antibodies only interacted with one epitope rather than other epitopes. The purity of the antibody reached 95% and the mean recovery rate was 5.8%., Conclusion: The beads coated with MAP can be used to quickly isolate specific single-epitope antibody which is similar to monoclonal antibody. This method provide a convenient shortcut in preparing small amount of antibody with high specificity.

To identify up-regulated genes in adult rat cardiac fibroblasts (CF) induced by angiotensin II (Ang II), suppression subtractive hybridization (SSH) was performed between the CF stimulated by Ang II (tester) and unstimulated CF (driver) to generate subtractive cDNA library. The library was screened with dot blots hybridization to further verify the differentially expressed cDNA clones. Partial positive clones (19 up-regulated genes) were sequenced and BLAST analyzed. Twelve up-regulated genes related to extracellular matrix, cell cycle, intracellular signal transduction, cell cytoskeleton, cell metabolism and 7 new expressed sequence tags (EST) were acquired (GenBank accession number: CN382808, CN382809, CN382810, CN382811, CN382812, CN382813, CN382814). Our data reveal that SSH is a powerful technique of high sensitivity for the detection and cloning of up-regulated genes expressed in CF induced by Ang II, which may be helpful to clarify the mechanism of cardiac remodeling.