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A two-degree-of-freedom collision system with gap and elastic constraint was used as the research object, and the regional distribution and transitions of the periodic motion of the system under low frequency conditions were studied by numerical simulation.It was obtained that with the decrease of excitation frequency, the p/1 periodic motion occurs with grazing bifurcation transitions to （p+1）/1 periodic motion.When the number of collisions was large enough, the Chattering-impact characteristics were presented.Then a circuit model that was completely equivalent to the collision system was established and simulation experiments were carried out.The results show that the results generated by establishing the equivalent circuit are consistent with those generated by numerical simulations, and the equivalent circuit is more efficient in simulation experiments, which can realize fast modal transitions and parameter adjustments，making it more convenient and providing a research method for the study of nonlinear dynamics.

Compared to traditional gas separation methods， membrane separation technology has advantages in low cost， easy operation， and high separation efficiency. In this study， the whisker mullite hollow fiber membrane （M0） with excellent high-temperature resistance， high strength， and large flux was used as the supporting layer. The PDMS with controllable viscosity was used as the intermediate transition layer. The PDMS coated on the surface of M0 and covered the defects on the substrate. Subsequently， the UiO-66-NH2 nanoparticles were modified with amino groups via the hydrothermal method. Trimesoyl chloride was used as the oil-phase monomer， and cysteamine was used as the water-phase monomer for interfacial polymerization. Through this interfacial polymerization process， UiO-66-NH2 was loaded on the top layer of the composite membrane to form the M0-PUi composite membrane. The properties of the membrane before and after modification were characterized by FT-IR， XRD， SEM， and water contact angle of the membrane surface. The CO2/N2 separation performance of the membrane was tested using a self-made gas separation device. The results show that the CO2 permeance of the composite membrane reaches 2765.3 GPU at room-temperature and under 0.2 MPa operating pressure， and its separation selectivity for CO2/N2 is 3.2. The stable separation selectivity of the composite membrane for CO2/N2 is 3.2-3.4 after continuous use at 80 ℃ or 120 ℃ for 6 h.

Objective·To explore the effects and possible molecular mechanisms of nanoplastics (NPs) on severe asthma.Methods·A mouse model of severe asthma was established by using house dust mite (HDM) and lipopolysaccharide (LPS) co-stimulation. Polystyrene nanoplastics (PS-NPs) were instilled into the severe asthma mice′s airways. Subsequently, bronchoalveolar lavage fluid (BALF) was collected and lung tissue sections were prepared. Flow cytometry, hematoxylin-eosin (H-E) staining, periodic acid-Schiff (PAS) staining, immunohistochemistry, and terminal dexynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) staining, were used to observe the effects of PS-NPs on airway inflammation, mucus secretion, alveolar structure, and the proliferation and apoptosis of alveolar type Ⅱ epithelial cells (AT2 cells) in severe asthma mice. The CCK-8 assay and Annexin Ⅴ/PI double staining were performed to evaluate the effects of PS-NPs on the proliferation and apoptosis of the mouse AT2 cell line MLE-12. DNA damage in AT2 cells caused by PS-NPs was detected by using anti-γ-H2A.X immunofluorescence staining. The expression of genes in the ATR/Chk1/p53 signaling pathway was detected by real-time fluorescent quantitative polymerase chain reaction (qPCR), Western blotting, Tyramide signal amplification (TSA) multiplex immunofluorescence staining, and immunofluorescence co-localization, respectively. The ATR-specific inhibitor Ceralasertib (AZD6738) was administrated to MLE-12 cells in combination with PS-NPs to evaluate the recovery effect on cell proliferation and apoptosis.Results·Flow cytometry revealed that exposure to PS-NPs increased the total number of inflammatory cells and the number of each type of inflammatory cells in the BALF of mice with severe asthma, with a predominance of neutrophils. H-E and PAS staining showed significant increase in airway inflammatory cell infiltration and mucus secretion, as well as disruption of alveolar structure. In vitro, the CCK-8 assay demonstrated significant, dose-dependent inhibition of MLE-12 cell proliferation by PS-NPs. The Annexin V/PI double staining assay indicated a higher apoptosis rate of (56.20±3.84)% in PS-NP-exposed cells compared to (23.22±2.52)% in the control group. Immunofluorescence staining demonstrated that PS-NPs were phagocytosed by MLE-12 cells and localized around the nucleus. TUNEL staining confirmed enhanced apoptosis in AT2 cells in vivo. The immunofluorescence assay revealed that compared to the control group, the expression of the DNA damage marker γ-H2A.X increased in the experimental group. qPCR, Western blotting, and TSA multiplex staining results showed that PS-NP-induced elevated expression of mRNA and proteins was related to the ATR/Chk1/p53 pathway in MLE-12 cells. Moreover, immunofluorescence co-localization also confirmed the induction of ATR and p53 proteins in AT2 cells in vivo. The ATR-specific inhibitor Ceralasertib partially restored the PS-NP-induced inhibition of cell proliferation and enhancement of apoptosis in MLE-12 cells.Conclusion·NPs exposure leads to DNA damage in AT2 cells, activating the ATR/Chk1/p53 signaling pathway and exacerbating airway inflammation and alveolar damage in mice with severe asthma.

Background While the connection between monounsaturated fatty acid (MUFA) intake and non-alcoholic fatty liver disease (NAFLD) has received attention, research in Chinese populations remains scarce. Objective This study aims to evaluate the association between daily MUFA intake and the prevalence of NAFLD in a Chinese population and to estimate the probable threshold of MUFA intake for NAFLD risk. Methods This case-control study employed data from the Dryad database (https://doi.org/10.5061/dryad.8nn2j46), complying with Dryad's terms of service. We collected data from 534 NAFLD patients and 534 healthy controls who underwent medical tests at the First Hospital of Nanping Affiliated to Fujian Medical University between April 2015 and August 2017. A semi-quantitative food frequency questionnaire (SQFFQ) was used to collect individuals' typical food consumption information, and daily MUFA intake was computed. A thorough analytical technique, including baseline characteristics, multivariate statistics, non-linear relationship analysis, and subgroup research, was applied to study the correlation between daily MUFA intake and NAFLD prevalence. Multiple confounding factors, such as age, sex, lifestyle, and other dietary components, were evaluated to estimate the theoretical threshold of MUFA intake for NAFLD risk. Results In the adjusted model, daily MUFA intake was a significant risk factor for NAFLD (OR=1.04, 95%CI=1.02-1.07, P

Aiming at the problems of existing time synchronization accuracy measurement methods based on clock pulse, such as complex measurement system composition, limited measurement scale by the number of instrument acquisition interfaces, and inability of measurement data to support real-time network management and control, a lightweight and scalable time synchronization accuracy measurement method called Virtual Scoping (VS) was proposed based on the centralized management and control architecture of time-sensitive networking (TSN). Ability to virtualize oscilloscope functions in the network control plane. Firstly, the parameter DriftTime was defined by the method, which could be used to accurately estimate synchronization accuracy. This parameter was calculated from protocol status data such as synchronization message transmission timestamps, residence delay, and link delay. Finally, the V-Scope tool was implemented in the network control plane and deployed in a prototype system built on the OpenTSN open-source project for experimentation. The results demonstrate that V-Scope exhibits only nanosecond-level errors compared to measurements obtained from professional testing equipment. Additionally, the feasibility and advantages of the VS method in supporting network management functions are discussed. As an example of accuracy optimization, a frequency offset compensation algorithm based on V-Scope is designed and implemented, which improves the synchronization accuracy of the prototype system by 32.3%.

ObjectiveTo investigate the clinical manifestations, molecular genetics and gonadal pathology characteristics of patients with disorders of sex development (DSD), and to summarize the clinical experience of identifying rare diseases from common symptoms.MethodsThe clinical data of 416 patients with DSD diagnosed and treated in the multidisciplinary center of DSD of Guangzhou Women and Children′s Medical Center from May 2018 to August 2023 were retrospectively analyzed, summarized and discussed.ResultsAccording to chromosome karyotype, 416 cases of DSD were classified into three types: 92 cases(22.1%) of abnormal sex chromosome karyotype, 285 cases(68.5%) of 46, XY karyotype and 39 cases(9.4%) of 46, XX karyotype. Among the 92 patients with abnormal sex chromosome karyotype, 59 cases were raised as males, 18 cases (30.5%) complained of short penis with hypospadias and cryptorchidism. The most common karyotype was 45, X/46, XY(58 cases, 63.0%).Among the 285 patients with 46, XY karyotype, 238 cases were raised as males, and 63 cases(26.5%)complained of short penis and hypospadias; 47 cases were raised as females, and 13 cases(27.7%) complained of inguinal mass. A total of 216 patients with 46, XY karyotype were subjected to whole exome gene detection, and 155 cases(71.8%) were found to have molecular pathogenesis with the clinical phenotype. Among the 39 patients with 46, XX karyotype, 19 cases were raised as males, and 8 cases(42.1%) complained of short penis and hypospadias. In the 18 cases of gonad biopsy, 17 cases showed testicular tissue in gonads. Whole exome sequencing was performed in 14 cases. NR5A1 gene heterozygous mutation, SRY gene mutation and SOX3 gene mutation were found in 2 cases, respectively(14.3%). Twenty cases were raised as females, and 14 cases(70.0%) complained of clitoral hypertrophy. Gonad biopsy was performed in 8 cases, with 7 cases of ovotestis(87.5%) and 1 case of NR5A1 gene heterozygous mutation(14.3%).ConclusionsThe etiologies of DSD are complex and diverse, and the clinical manifestations are various, which can be manifested as hypospadias, micropenis, cryptorchidism and other common symptoms of the urinary system. Different etiologies have different treatment options. Therefore, chromosome karyotype, molecular genetic testing and gonadal pathology can be used to clarify the cause of disease, especially for rare diseases, improve the detection rate, reduce the rate of missed diagnosis, and ensure reasonable treatment, especially sex selection.

The large number of network elements in the mega-constellations pose a severe challenge to the existing network architecture and technical system.Based on the concept and characteristics of constellation architecture, combined with the development of domestic and foreign satellite constellations and technology prediction, a multi-layer and multi-domain hybrid intelligent cooperative network architecture was proposed, which consist of four layers (perception plane, data plane, control plane, intelligent management plane) and three layers (resource layer, transport layer, service layer) , the architecture of constellation network management and control, technology reference model and protocol architecture were designed.At the same time, accorded to the construction and development trend of constellation, this paper expounded and analyzed the evolution trend of the key technologies, such as constellation network addressing and routing, network robust agile guarantee, differentiated service bearing, end-to-end reliable transmission, etc.The research results could support the development and construction of LEO giant constellation network in our country.

Diabetic ulcer (DU), one of the common and serious complications in patients with diabetes mellitus, often leads to infection, necrosis and amputation, and has a long and costly treatment period. Because of DU's unclear healing mechanism and the difficulty of delayed healing, its treatment and management have been a major challenge in clinical medicine. In recent years, the potential role of mitochondrial autophagy in DU has become a research hotspot with the in-depth study of mitochondrial autophagy mechanism. Previous studies have shown that mitochondrial autophagy is an important intracellular self-repair mechanism that plays a crucial role in maintaining cellular health and functional stability. During the development of DU, mitochondrial autophagy plays multiple roles in attenuating oxidative stress and inflammatory responses, maintaining mitochondrial functional homeostasis, influencing cell proliferation and repair capacity during DU healing, promoting DU healing, and enhancing antimicrobial capacity. In this paper, we illustrate the multiple roles played by mitochondrial autophagy in DU prevention and treatment, as well as the potential applications of mitochondrial autophagy in DU therapy. It is expected to provide a basis for the clinical application of mitochondrial autophagy in DU treatment, and provide more effective strategies and solutions for the treatment of DU.

In the context of achieving “carbon peaking and carbon neutrality”, the low-carbon transformation of the energy system is the development direction in the future. Hydrogen, known for its high calorific value and low pollution, has received extensive attention in recent years. Based on the carbon emission flow theory, a bi-level optimization operation model of multi-integrated energy system with hydrogen (H2-IES) is proposed considering dynamic carbon emission factors. At the upper level, an economic dispatch model is established by the main energy grid based on the principle of optimal benefit, and the energy prices and carbon emission factors of each park are determined and distributed to the lower level. At the lower level, a multi-park low-carbon cooperative operation model is established based on the Nash negotiation theory, and the adaptive alternating direction method of multipliers (A-ADMM) is used for distributed solution to determine the energy demand of each park and provide feedback to the upper level. The coordinated operation of both levels is realized in multiple iterative interactions. To equitably distribute the benefits of cooperation, a revenue distribution method based on comprehensive bargaining power is proposed. The analysis of a case study shows that the bi-level optimization method proposed in this paper can realize the coordinated operation between the upper and lower levels, and take into account the low-carbon and economical properties of multi-parks operation. Because the income is reasonably distributed, the enthusiasm of parks to participate in cooperation can be guaranteed.

Objective To explore the impact of the sialic acid binding lectin-E (Siglec-E) on the inhibitory properties of parthenolide(PTL) against lipopolysaccharide (LPS)-induced M1 polarization of microglia(BV2). Methods ① Single cell sequencing data of Siglece related mouse brain tissue was obtained from Gene Expression Omnibus (GEO) database and divided into the WT group (n=3) and the Siglece-/- group (n=4). The microglia cells were screened, and the enrichment analysis was performed to analyze related differential genes and pathways. BV2 cells were constructed by the shRNA interference technique and were divided into NC-shRNA and Siglece-shRNA to detect the expression level of Siglec-E (Siglece). ② NC-shRNA and Siglece-shRNA cells were respectively divided into the Control group, LPS group, PTL group and PTL+LPS group (n=3). The mRNA levels of markers of M1 polarization in microglia, iNOS, IL-1β and IL-6, were detected by RT-qPCR. Siglecefl/fl and Cx3cr1cre mice were mated to obtain microglia-specific Siglece deletion (Siglecefl/fl×Cx3cr1cre) mice, and LPS-induced neuroinflammation model was established. ③ Nine WT and Siglecefl/fl×Cx3cr1cre male mice were assigned to the Control group, LPS group and PTL+LPS group (n=3). RT-qPCR, immunofluorescence assay and Western blotting were used to verify the knock-out effect and polarization-related pathways, and to investigate the mechanism of Siglec-E affecting PTL inhibition of M1 polarization of microglia. Results Compared with the NC-shRNA group, the expression of Siglec-E in the Siglece-shRNA group was significantly decreased (P < 0.01), indicating that the Siglec-E knock-down cell model was successfully established. With the stimulation of LPS, mRNA levels of iNOS, IL-1β and IL-6 were significantly up-regulated compared with the Control group both in shRNA cells and Siglece -shRNA cells (P < 0.01). With the influence of PTL and LPS, the markers of M1 polarization in NC-shRNA cells mentioned before were significantly decreased (P < 0.05), while for Siglice-shRNA cells, there were no significant changes in the markers of M1 polarization. PTL inhibited the phosphorylation of JNK and IκB protein (P < 0.01) and the nuclear translocation of NF-κB in BV2 cells, down-regulated Siglec-E, and weakened the inhibitory effect. Compared with mice in the WT group, the expression of Siglec-E in microglia of Siglecefl/fl×Cx3cr1cre mice was decreased significantly (P < 0.01), and the inhibitory effect of PTL on the phosphorylation of NF-κB in microglia of Siglecefl/fl×Cx3cr1cre mice was also decreased. Conclusion The absence of Siglec-E in microglia attenuates the inhibition of M1 polarization by the MAPK/NF-κB pathway targeted by PTL.

The Qinghai-Xizang Plateau has complex terrain and climate， which is a great challenge to the airdrop parachute landing and aviation safety.This research focuses on Qinghai-Xizang Plateau wind field simulation in boundary layer based on numerical calculation method.Firstly， the study built a WRF-LES system and scaled down to 40 m horizontal resolution based on the large eddy simulation （LES） scheme of WRF （Weather Research and Forecasting） model， and undertook the application study of large eddy simulation on the Qinghai-Xizang Plateau.Based on a strong wind case over the Qinghai-Xizang Plateau， the impacts of LES scheme and terrain elevation data on wind field simulation were evaluated through sensitivity tests.Then， the parameters in the standard sub-grid turbulent stress models of LES scheme were analyzed， and the optimal schemes for wind field simulation on the Qinghai-Xizang Plateau was obtained.Finally， a batch test was conducted to verify the applicability of the optimal schemes to the Qinghai-Xizang Plateau wind field simulation.The test results show that the WRF-LES system with a resolution of 40 m can simulate more precise and accurate wind field information， and the MAE （Mean Absolute Error） of simulated wind speed is reduced by 1.4 m·s-1 and the RMSE （Root Mean Square Error） is reduced by 1.81 m·s-1 compared with the ACM2 scheme； The high-precision ASTER terrain data can also improve the effect of wind field simulation， and the error is approximately deduced by 0.2 m·s-1； The LES scheme that use 1.5-order turbulent flow energy scheme and set parameter coefficient 0.1 has the best simulation result， and the MAE is 1.56 m·s-1 and RMSE is 2.06 m·s-1； The batch test verifies that the large eddy simulation scheme is fit for the wind simulation on the Qinghai-Xizang Plateau， and the wind field simulation results in the 40 m resolution is significantly better than in the mesoscale resolution.The result shows that WRF-LES system can provide accurate wind field information for the parachute landing on the plateau.

Objective Sb deposits are characterized by simple mineral assemblage. The ore-forming ages, sources of ore-forming materials, and genesis of Sb deposits are controversial owing to the absence of suitable minerals for analysis. Sb resources in the South China Sb metallogenic region account for over 83% of the national total, with the Dian–Qian–Gui Sb belt in the southwest being an significant component of this region. Methods Taking the Longlin–Xilin Sb–Au mining district of western Guixi in the central part of the Dian–Qian–Gui Sb belt as an example, this paper systematically summarizes the ore-bearing strata, lithology of ore-bearing wall rocks, ore-bearing structures, and the coexistence relationship of Au and Sb deposits in 86 ore deposits (points) in the area. Combined with the geological characteristics of three typical deposits (Maxiong, Longtan, and Mahao) and the spatiotemporal distribution of Jurassic felsic intrusions, the inherent connection between Sb mineralization and clastic rocks and felsic intrusions was explored. Results (1) Statistics and field works show that the most favorable ore-bearing stratum in the Longlin–Xilin mining district is the Lower Devonian Yujian Formation (D1y) , followed by the Lower Triassic Luolou Formation (T1Ll) and the Middle Triassic Banna Formation (T2b). The lithologies most conducive to mineralization are carbonaceous shale, pyrite-rich sandstone, and siltstone. The Sb content in these strata or lithologies is tens or even hundreds of times higher than the crustal abundance, which has the potential for Sb mineralization. (2) Within the NWW–SEE trending Nongsang–Shijiazhai fault zone in the Longlin area, the middle and late Jurassic felsic intrusions, which have consistent spatiotemporal occurrences with Sb and Au deposits, can directly contribute to antimony mineralization (as sources of Sb and S) and indirectly influence it (as a heat source), both favoring the formation of antimony deposits. (3) Statistical results show that Sb, Au, and Sb–Au deposits account for 48%, 46%, and 6% in the Longlin–Xilin district, respectively. This suggests that the ore-forming fluids for Sb and Au in the study area may originate from different sources. We also can not rule out the possibility that Sb and Au deposits derive from the same fluid. In the latter case, the precipitation of stibnite consumes H2S in the ore-forming fluid, destabilizing the Au complex in the solution and resulting in localized Au precipitation. This competition between Sb and Au in the fluid for H2S leads to a negative correlation in the grades of Sb and Au in coexisting deposits. (4) The study area experienced NS-striking compression in the Indosinian period, followed by the NW–SE shortening in the middle–late Jurassic. The intersection of NWW–SEE and NE–SW faults is the favorable ore-bearing space. The NWW–SEE faults displayed strike-slip movement in response to the NW–SE shortening, whereas the NE–SW faults exhibited transpression. Consequently, the NE–SW faults are less conducive to Sb mineralization compared to the NWW–SEE faults. The distribution direction of the NWW–SEE Douhuang–Xilin fault aligns with the axial direction of the main folds in the area, with most fault planes trending northward, displaying horizontal scratches, silicification, and extensional characteristics. The intersection of the Dohuang–Xilin fault and the NE–SW fracture exhibits significant Sb anomalies. Conclusion Based on the above studies, the promising areas we propose for Sb prospecting in Longlin–Xilin mining district are (1) Black shale and pyrite-rich siltstones of the Yujiang Formation in the core of the Xinzhou anticline as the key strata; (2) The periphery of the concealed intrusions within the NWW–SEE Nongsang–Shijaizhai fault (Longlin County) and the intersection area of the NWW–SEE Douhuang–Xilin fault and the NE–SW fault as the favorable areas. [ Significance ] The findings provide new insights into the genesis and metallogenic regularities of Sb–Au deposits in the study area, enriching the theoretical understanding of Au mineralization processes.

Cutting fluid wastewater contains a large amount of emulsified oil. If it is discharged directly without treatment, it will cause environmental pollution and water ecosystem destruction. In order to efficiently separate oil-in-water emulsion wastewater and reduce membrane fouling, polyvinylidene fluoride (PVDF) hollow fiber membrane with good membrane-forming property, stable mechanical properties and good chemical resistance was used as the support, the amino group of cysteamine was introduced to the membrane surface, and then amidated with the carboxyl group of methacrylic acid, and followed by forming a cyclic polymer functional layer through a free radical polymerization reaction, thus PVDF-based composite membrane with good hydrophilicity, large flux and high oil-water separation rate was obtained. The physical and chemical properties of the modified films were characterized by infrared spectroscopy (IR), scanning electron microscopy (SEM) and water contact angle. The separation effect of n-hexane-in-water emulsion and cutting fluid wastewater was tested by self-made cross-flow filtration device. The results show that the water contact angle (WCA) of the original membrane (M0) is 91.95°, while the WCA of the modified membrane M1, M2 and M3 decrease to 65.78°, 51.08° and 71.70°, respectively, indicating that the hydrophilicity of the modified membrane is improved. The permeation fluxes of M0, M1, M2 and M3 for n-hexane-in-water emulsion are 37.34, 58.40, 81.57, 44.55 L·m-2·h-1, respectively. The water fluxes for cutting fluid and wastewater separation are 7.41, 11.96, 24.36, 10.80 L·m-2·h-1, respectively. The fluxes of the original membrane and the modified membrane in separating high concentration cutting fluid wastewater decrease, but the permeation flux of the modified membrane is obviously higher than that of the original membrane, and the permeation flux of M2 modified membrane is the best, which indicates that the modified membrane has the best hydrophilicity and anti-pollution ability, and is expected to be used in the treatment of industrial oily wastewater.

[Objective]The usual agricultural machinery navigation focuses on the tracking accuracy of the tractor, while the tracking effect of the trailed implement in the trailed agricultural vehicle is the core of the work quality. The connection mode of the tractor and the implement is non-rigid, and the implement can rotate around the hinge joint. In path tracking, this non-rigid structure, leads to the phenomenon of non-overlapping trajectories of the tractor and the implement, reduce the path tracking accuracy. In addition, problems such as large hysteresis and poor anti-interference ability are also very obvious. In order to solve the above problems, a tractor-implement path tracking control method based on variable structure sliding mode control was proposed, taking the tractor front wheel angle as the control variable and the trailed implement as the control target.[Methods]Firstly, the linear deviation model was established. Based on the structural relationship between the tractor and the trailed agricultural implements, the overall kinematics model of the vehicle was established by considering the four degrees of freedom of the vehicle: transverse, longitudinal, heading and articulation angle, ignoring the lateral force of the vehicle and the slip in the forward process. The geometric relationship between the vehicle and the reference path was integrated to establish the linear deviation model of vehicle-road based on the vehicle kinematic model and an approximate linearization method. Then, the control algorithm was designed. The switching function was designed considering three evaluation indexes: lateral deviation, course deviation and hinged angle deviation. The exponential reaching law was used as the reaching mode, the saturation function was used instead of the sign function to reduce the control variable jitter, and the convergence of the control law was verified by combining the Lyapunov function. The system was three-dimensional, in order to improve the dynamic response and steady-state characteristics of the system, the two conjugate dominant poles of the system were assigned within the required range, and the third point was kept away from the two dominant poles to reduce the interference on the system performance. The coefficient matrix of the switching function was solved based on the Ackermann formula, then the calculation formula of the tractor front wheel angle was obtained, and the whole control algorithm was designed. Finally, the path tracking control simulation experiment was carried out. The sliding mode controller was built in the MATLAB/Simulink environment, the controller was composed of the deviation calculation module and the control output calculation module. The tractor-implement model in Carsim software was selected with the front car as a tractor and the rear car as the single-axle implement, and tracking control simulation tests of different reference paths were conducted in the MATLAB/Carsim co-simulation environment.[Results and Discussions]Based on the co-simulation environment, the tracking simulation experiments of three reference paths were carried out. When tracking the double lane change path, the lateral deviation and heading deviation of the agricultural implement converged to 0 m and 0° after 8 s. When the reference heading changed, the lateral deviation and heading deviation were less than 0.1 m and less than 7°. When tracking the circular reference path, the lateral deviation of agricultural machinery tended to be stable after 7 s and was always less than 0.03 m, and the heading deviation of agricultural machinery tended to be stable after 7 s and remained at 0°. The simulation results of the double lane change path and the circular path showed that the controller could maintain good performance when tracking the constant curvature reference path. When tracking the reference path of the S-shaped curve, the tracking performance of the agricultural machinery on the section with constant curvature was the same as the previous two road conditions, and the maximum lateral deviation of the agricultural machinery at the curvature change was less than 0.05 m, the controller still maintained good tracking performance when tracking the variable curvature path.[Conclusions]The sliding mode variable structure controller designed in this study can effectively track the linear and circular reference paths, and still maintain a good tracking effect when tracking the variable curvature paths. Agricultural machinery can be on-line in a short time, which meets the requirements of speediness. In the tracking simulation test, the angle of the tractor front wheel and the articulated angle between the tractor and agricultural implement are kept in a small range, which meets the needs of actual production and reduces the possibility of safety accidents. In summary, the agricultural implement can effectively track the reference path and meet the requirements of precision, rapidity and safety. The model and method proposed in this study provide a reference for the automatic navigation of tractive agricultural implement. In future research, special attention will be paid to the tracking control effect of the control algorithm in the actual field operation and under the condition of large speed changes.

ObjectiveTo investigate the implementation of the standards （formula foods and complementary foods for infants and young children）， the contents of glucose， galactose， fructose， lactose， sucrose and maltose in formula foods and complementary foods for infants aged 0-36 months， and provide data support for the management of food safety standards related to infant formula and infant complementary food in China.MethodsBased on the principle of random sampling， 83 formula food and complementary food for infants and young children， including infant formula foods， older infant formula foods， young children formula foods， cereal complementary foods and canned foods for infants and young children， were collected from the domestic market， the content of glucose， galactose， fructose， lactose， sucrose and maltose were determined by ion chromatography-pulse ampere method and the results were analyzed.ResultsThe median of total sugar content in formula foods for infants and young children was 433.43-476.99 g/kg； the proportion of lactose in all kinds of products was higher than 95%， and the content from high to low was infant formula， older infant formula and formula for young children. The detection rate of sugar in infant cereal supplementary food was 36.8%， the median content was 0-28.35 g/kg； the medians of lactose and sucrose were 84.30 and 70.17 g/kg； while the third quartile（Q3） of sucrose was 53.43 g/kg. There was no significant difference in the content of added sugar between domestic and imported formula foods（P>0.05）.ConclusionThe lactose content in formula foods for infants and young children sold in China is higher than that of other sugars. The content of total sugar in cereal supplementary food is low， but the content of sucrose in biscuit should be concerned. It is suggested that enterprises should strictly control added sugar， and government departments should continue to push the implementation of the sugar content regulation in the newly released national standards.

Nonalcoholic fatty liver disease (NAFLD), the most common chronic liver disease, represents a spectrum of nonalcoholic hepatic steatosis, nonalcoholic steatohepatitis, liver cirrhosis and hepatocellular carcinoma. Recent studies have revealed that the gut-liver axis is associated with the development of NAFLD. Intervention measures such as exercise, diet, microecological preparations, antibiotics, fecal microbiota transplantation and bacteriophage can alleviate NAFLD by improving intestinal microbiota. We reviewed recent advances in the relationship and mechanism between intestinal microbiota and NAFLD as well as treatment of NAFLD targeting the intestinal microbiota, and summarized that intestinal microbiota is closely related to NAFLD, and the regulation of intestinal microbiota can be used as a new target for NAFLD treatment, which will provide new ideas and references for clinicians in the diagnosis and treatment of NAFLD.

ObjectiveTo observe the effect of Jin's three-needle therapy combined with standing digital OT training on upper extremity motor function of patients with hemiplegia after stroke.MethodsA total of 60 patients with upper extremity hemiplegia after stroke in the inpatient department of acupuncture-moxibustion and rehabilitation of Guangdong Second Traditional Chinese Medicine Hospital from January to June 2021 were divided randomly into control group and observation group, with 30 cases in each group. Both groups received routine medication and routine rehabilitation training. The control group received Jin's three-needle therapy on the basis of routine medication and routine rehabilitation training, and the three-needle points were selected at the contralateral temporal, the affected shoulder and hand [Quchi (LI11), Waiguan (TE6), Hegu (LI04)], Baihui (DU20) and Shuigou (DU26) acupoints. The even reinforcing-reducing method was conducted, and when the patients brought about the desired sensation, the needle was retained for 30 minutes, once a day, six times a week, lasting for eight weeks. The observation group received stan-ding digital OT training on the basis of the control group, 20 minutes a time, once a day, six times a week, lasting for eight weeks. Before treatment and eight weeks after treatment, Fugl-Meyer assessment-upper extremity (FMA-UE) was used to evaluate upper extremity motor function; Wolf motor function test (WMFT) was used to quantify the upper extremity motor ability; modified Barthel index (MBI) was used to assess the ability of activities of daily living.ResultsCompared with that before treatment, the FMA-UE, WMFT and MBI scores of both groups increased significantly after treatment (PP

ObjectiveTo investigate the capacity of silkworm cocoon water (SCW) to mitigate the level of oxidative stress in aged rats.MethodsThe samples were processed by simulating the traditional SCW production process, and the SCW components were examined. The 16-month-old rats were randomly divided into 3 experimental groups that were treated with different doses of SCW, and 1 negative control group according to serum malonaldehyde (MDA) content, with 10 rats in each group. Experimental groups were fed SCW at doses of 500, 250, 125 mg/kg body weight, respectively, and the negative control group was given pure water, once a day, for 30 days. At the end of the experiment, the contents of lipid oxidation products (MDA), protein oxidation products (carbonyl), antioxidant enzyme activities (SOD, CAT, GSH-Px), and antioxidant substances (GSH) were measured.ResultsThe crude protein content of SCW was 1 640 mg/100 g, the free amino acid content was 18 mg/100 g, and the hydrolyzed amino acid content was 1 700 mg/100 g. Serine (30.59%), aspartate (18.82%), glycine (10.00%), and threonine (8.24%) were the main hydrolyzed amino acids in SCW. Compared with the negative control group, SCW reduced the contents of MDA and protein carbonyl in the serum and tissue (P < 0.05), but increased the contents of GSH in the serum and tissue, the activity of SOD in tissue, and the activity of CAT and GSH-Px in the serum and tissue of aged rats (P < 0.05).ConclusionSCW has the effect of improving oxidative stress in aged rats.

【Objective】In order to improve the growth and immune performance of Hainan black goat, a new type of immune synergist was developed with Noni (Morinda citrigolia L.) as the material to promote the healthy breeding and sustainable industry development of Hainan black goat.【Method】Fifty-four healthy Hainan black goats were selected and randomly divided into 6 groups. The experimental period was 60 d, and the growth and immune performance of each group were measured on the 0 d, 30 d and 60 d of the experiment, respectively.【Result】On the 60th day of the experiment, the average body weight and daily gain of the high-dose Noni group were significantly different from those of the Astragalus polysaccharide and other groups, and increased with the increase of the supplemental dose. In addition, there was no significant difference in serum total protein and globulin between Astragalus p. group and Noni group. On the 30th day and 60th day of the test, the serum levels of IgG, IL-2 and IFN-γ in the high-dose Noni group were slightly higher than those in the other treatment groups, which were significantly different from those in the control group, and they were increased with the increase of the additive dose.【Conclusion】Adding 20 g Noni powder every day and feeding for 60 days is the ideal feeding scheme, and the daily gain of Hainan black goat can reach 97.27 (±20.87) g. The levels of IgG, IL-2 and IFN-γ are 49.17 (±3.52) g/L, 136.24 (±12.77) ng/L and 112.72 (±10.94) ng/L, respectively. Noni can significantly improve the growth and immune performance of Hainan black goat. Therefore, Noni has a broad application prospect in the green and healthy breeding and industrial development of Hainan black goat.

In order to study the effects of the surface microstructure of micro arc oxidation (MAO) coating on the structure and properties of MAO/ diamond ⁃like carbon (DLC) film composite coating, MAO coatings with different roughnesses were prepared on the surface of magnesi⁃ um alloy by voltage regulation, and then MAO/ DLC composite coatings were constructed by unbalanced magnetron sputtering. The microstructure, roughness, thickness, mechanical properties, friction and wear properties and corrosion resistance of the coatings were studied by scanning electron microscope (SEM), electron energy dispersive spectroscopy (EDS), surface roughness tester, scratch tester, friction and wear tester and electrochemical workstation. Results showed that when the roughness of MAO coating increased gradually from 0.19 μm to 0.57μｍ, the surface porosity of MAO coating increased from 9.429% to 12.924%, the friction coefficient increased from 0.53 to 0.66, and the self⁃corrosion potential shifted negatively from -1.571 V to -1.595 V. Moreover, the roughness of 3.1 μm thick DLC film deposited on the surface increased from 0.22 μm to 0.58 μm. The surface porosity increased from 1.553% to 6.074%, the friction coefficient increased from 0.24 to 0.45, the wear rate increased from 2.01×10-5 mm3/(N·m) to 8.41×10-5 mm3/(N·m), and the self ⁃ corrosion potential shifted positively from -1.547 V to -1.486 V. Meanwhile, the interfacial bonding strength of the composite coating increased from 5.51 N to 7.89 N, and the bonding force increased by 43%. Overall, the surface roughness of MAO coating determined the interface bonding strength with DLC coating. Increasing the surface roughness could significantly improve the interface bonding strength of composite coating, however it would also increase the surface porosity, resulting in the deterioration of corrosion / wear protection performance of MAO coating and its composite coating.

ObjectiveThe previously established 38-plex InDel system was optimized and its performance was validated according to the Scientific Working Group on DNA Analysis Method （SWGDAM） application guidelines. The ancestry inference accuracy of individuals from East Asian， European， African and mixed populations was verified.MethodsDNA standard sample 9947A was used as the template to establish the optimal amplification conditions by adjusting primer balance， Mg2+ final concentration and optimizing PCR thermal cycle parameters and amplification volume. The allelic dropout， nonspecific amplification and whether the origin of the inferred samples matched the known information were compared to evaluate the performance of this system.ResultsThe optimal dosage of this system was 0.125-2 ng DNA template. The results of InDel typing were accurate， the amplification equilibrium was good， and the species specificity was good. This system showed certain tolerance to DNA samples including the inhibitor such as hemoglobin （≤80 μmol/L）， indigo （≤40 mmol/L）， calcium ion （≤1.0 mmol/L）， and humic acid （≤90 ng/μL）. The system enabled the direct amplification of DNA from saliva and blood on filter paper， and the results of ethnic inference were accurate. The system successfully detected the mixed DNA sample from two individuals. The test results of the system for common biological materials in practical cases were accurate.ConclusionThe results of the 38-plex InDel system are accurate and reliable， and the performance of the system meets the requirement of the SWGDAM guidelines. This system can accurately differentiate the ancestry origins of individuals from African， European， East Asian， and Eurasian populations and can be implemented in forensic practice.

In order to ensure the stable operation of the miniature gastrointestinal robot inside the human body, a new type of wireless power transmission (WPT) system is presented. To fulfill the requirements of the work energy consumption, posture stability, energy supply stability, and size limitation of the intestinal robot, the design refers to the calculation of electromagnetic field theory and the optimization results of the spindle-shaped three-dimensional receiving coil model which based on the principle of electromagnetic field resonance. Accordingly, the transmitting coil adopts four sets of solenoid coils to generate a uniform alternating magnetic field. With the receiving coil integrated inside, the optimized transmitting coil can generate a stable energy greater than 500 mW for a robot to work through mutual inductance. Relevant experimental verification indicates that performance of the wireless energy supply system satisfies the working requirements of the robot in the complex environment inside the intestine.

This paper analyzes the main causes of accidents during wellbore construction and the existing problems of safety monitoring technology, puts forward safety monitoring robot technology for wellbore construction based on negative pressure adsorption, and analyzes the basic functions of safety monitoring robot in detail. The research goal of wellbore construction safety monitoring robot based on negative pressure adsorption is proposed from the robot body structure, walking mechanism, adsorption mechanism, control, communication, intelligent sensing, identification of dangerous sources, identification of explosive detonators, software platform development, etc. The technical device of wellbore construction safety supervision robot based on negative pressure adsorption is designed, the characteristics of the technology are defined.

【Objective】 Increasing and stabilizing carbon in coarse-textured soils is important to improve their stability and fertility. The purpose of this paper is to investigate how soil amendment with organic materials and clay minerals can achieve these goals. 【Method】 We took spinach as a model crop, and compared eight treatments by amending the soil with woody peat (HW), biochar (HB), lignite (HC), humic acid (HH), woody peat + bentonite (HWb), woody peat + illite (HWa) and woody peat + kaolinite (HWg), respectively. Not amending the soil was taken as the control (H0). In each treatment, we measured crop growth as well as the changes in soil physical and chemical properties. 【Result】 Compared with H0, HW, HB, HC and HH improved the growth and yield of the spinach, as well as physical and chemical properties of the soil. In particular, HB increased the net photosynthetic rate, plant height and leaf of the spinach during the harvest period by 21.5%, 23.5%, 69.5% and 70.9%, respectively, and soil organic matter and total nitrogen content by 236.2% and 20.7%, respectively, compared to H0. In comparison, HH reduced soil pH by 16.8% and increased crop yield by 4.7% during the harvest period, compared to H0. Combined with clay mineral amendment, HWb, HWa and HWg increased crop yield by 22.7%, 9.0%, and 9.0%, respectively, and soil organic matter by 33.5%, 2.4%, and 27.0%, respectively, compared with HW without clay mineral amendment. 【Conclusion】 Amending the soil with organic materials alone, biochar was most effective in improving soil fertility and crop yield. For combined amendment with organic materials and clay minerals, amending the soil with woody peat and swelling and shrinking clay mineral bentonite at 2:1 ratio worked best for boosting crop growth while in the meantime improving soil fertility.

Objective To synthesize ethylene glycol chitosan-diethylenetriamine pentaacetic acid (GC-DTPA), and evaluate its cytocompatibility and systemic toxicity within the dose range of routine use. Methods DTPA was grafted onto ethylene glycol chitosan (GC) by organic synthesis and chemical cross-linking method, and was subsequently characterized with proton nuclear magnetic resonance (1H NMR) and Fourier transform infrared (FT-IR) spectroscopy. CCK-8 assay was used to detect the effects of different concentrations of GC-DTPA on the proliferation of ICE-6 cells. In addition, 80 healthy SPF SD rats (aged 6~8 weeks, body weight 180~220 g) were randomly divided into 4 groups (n=20): control group (given normal saline), and experimental groups (60, 90 and 120 mg/kg GC-DTPA, respectively by intragastric gavage). The change of body weight and survival rate of rats in each group were observed. Blood routine test and blood biochemical examination were performed in the rats on the 14th day after gavage with aid of automatic blood humoral and biochemical immunoassay analyzers respectively. Then, the rats were sacrificed and the pathological changes of organs were observed after HE staining. Results FT-IR spectroscopy and 1H-NMR suggested that DTPA was successfully grafted onto GC, and GC-DTPA exhibited excellent cytocompatibility, with cell viability all above 90% after its treatment at different concentrations. In 14 d after administration, no significant differences were seen in the results of blood routine and blood biochemical tests, or in pathological changes of organs and tissues in each group. Conclusion The GC-DTPA synthesized by chemical synthesis and cross-linking method shows no obvious cytotoxicity, and no systemic toxicity is seen even after oral administration dose up to 120 mg/kg

ObjectiveAn online fully automated alkaline hydrolysis method for the determination of chloropropanol esters （MCPDE） and glycidyl esters （GE） in edible oils and fats was developed， and the effects of vegetable oil type and temperature on the conversion of GE to 3-chloro-1，2-propanediol （3-MCPD） were investigated.MethodsBased on the basic principles of AOCS Cd 29c-13， the samples were subjected to hydrolysis， chlorination， liquid-liquid extraction and derivatization on a fully automated sample pretreatment platform， and then separated by a DB-5MS column after injection. The data was determined by multiple reaction monitoring mode （MRM） coupled with the internal standard method.ResultsThe efficiency of GE conversion to 3-MCPDE in the five vegetable oils was significant different. However， the effect of temperature was relatively greater. The alkali hydrolysis time was optimized， and the hydrolysis of MCPDE and GE was basically completed when the hydrolysis was carried out for 7 min at 10℃. The limits of detection for 2-chloro-1，3-propanediol （2-MCPDE）， 3-chloro-1，2-propanediol （3-MCPDE） and GE were all 5 μg/kg calculated by their free forms. There was a good linear relationship between the concentration and ratio of the response ranged from 0 to 0.4 μg/mL. When spiked from 20 to 2 500 μg/kg， the recoveries of MCPDE and GE ranged from 90.3% to 109.7% with the relative standard deviation （n=6） of 1.3%-7.7%， indicating that the precision was better than that of the manual procedure. The method was comparable with the Chinese national standard method for food safety under reviewed and was successfully applied to the determination of FAPAS proficiency test samples.ConclusionThe novel automatic procedure has the advantages of simple operation and high sensitivity， which reduces the labor intensity， improves the detection efficiency and repeatability compared with the reported manual procedure， and can better meet the requirement for quantification of MCPDE and GE in edible oils and fats.

In order to solve the problem that the data distribution of the source domain and the target domain of rolling bearing is different in the variable working condition environment and the samples of the target domain do not contain labels, a fault diagnosis model of the rolling bearing based on the deep adaptive transfer learning network (DATLN) is proposed. Firstly, a domain-shared characteristic extraction network is built, and multiscale convolutional neural network (MSCNN) is used to suppress noise interference, so as to effectively extract local fault information contained in vibration signals. Secondly, combined with a bi-directional long short-term memory network (BiLSTM), the temporal characteristics in the local fault information are further learned. Finally, transfer learning is introduced to build a domain adaptive module with domain adversarial (DA) training combined with adaptive joint distribution (AJD) metrics. By maximizing the domain classification loss and minimizing the AJD distance, the source and target domain characteristic samples are aligned. The anti-noise experiment and transfer experiment are carried out on the open source CWRU data set and the measured data set of the mechanical fault platform respectively. The anti-noise experiments show the following points. ① The identification accuracy of MSCNN-BiLSTM network is above 99% in the noise-free environment, which shows that MSCNN-BiLSTM network has a good characteristic extraction capability. ② The identification accuracy of MSCNN-BiLSTM, LeNet-5, MSCNN and BiLSTM decreases with the increase of noise intensity. ③ Under the noise environment of 3, 5 and 10 dB, the average identification accuracy of MSCNN-BiLSTM network is higher than that of LeNet-5, MSCNN and BiLSTM networks, indicating that MSCNN-BiLSTM network has better anti-noise interference performance. ④ The MSCNN-BiLSTM network converges first with less fluctuation in both the noise-free environment and the 3 dB noise environment. The transfer experiments show the following points. ① The average identification accuracy of DA+AJD method is 97.36% on unlabeled target domain dataset, which is higher than that of Baseline, transfer component analysis(TCA) and domain adversarial neural network (DANN). ② On the test set confusion matrix, only one sample of the DA+AJD method is incorrectly identified, indicating that the DA+AJD method based on domain adaptation has better fault transfer diagnosis performance. ③ The t-SNE algorithm is used to visualize the processed source and target domain characteristic samples. The DA+AJD method only has a small number of rolling element fault and outer ring fault characteristic samples in the target domain that are incorrectly aligned to the inner ring fault characteristic samples area in the source domain. This result indicates that the DA+AJD method can effectively reduce the edge distribution and conditional distribution differences between the source domain and the target domain, and thus achieves better characteristic sample alignment.

Objective To investigate the effects of high expression of high mobility group protein B1 (HMGB1) on the invasion ability of glioblastoma (GBM) and the efficacy of temozolomide (TMZ) therapy. Methods The HMGB1 sequencing data of 319 GBM samples from TCGA database were gained for differential expression and survival analysis. Immunohistochemistry (IHC) was adopted to assess the expression of HMGB1 in 4 samples (3 glioma samples, with WHO grade Ⅱ, Ⅲ and Ⅳ, respectively, and 1 para-tumoral brain tissue as control) which were collected from our hospital's biobank (inpatients from May to October 2019, male, aged 40~88 years, median 58.5 years, being confirmed as glioma by histopathological examination after tissue resection, without radiotherapy or chemotherapy prior to surgery). Moreover, lentiviral vector was transfected to construct HMGB1 silenced GBM cell lines (U251 and LN229). Western blotting was performed to detect HMGB1 expression; CCK-8 and transwell assays were adopted to analyze the effect of HMGB1 on cell proliferation and invasion ability. Finally, the role of HMGB1 in TMZ therapy was evaluated by CCK-8 assay (TMZ powder was dissolved in dimethyl sulfoxide to prepare 100 mmol/L storage solution, and the final concentration used was 250, 500, 1 000 and 1 500 μmol/L, respectively). Results TCGA database showed that HMGB1 mRNA level was significantly upregulated in glioma tissue (P < 0.01), increasing with WHO grade, and was negatively correlated with the prognosis of patients (P < 0.01). HMGB1 silenced GBM cells had decreased proliferation (P < 0.05) and inhibited invasion ability (P < 0.01), and were more sensitive to TMZ therapy. Conclusion HMGB1 is remarkably up-regulated in GBM cells, and its high expression contributes to the enhanced invasion ability and temozolomide resistance of GBM cells.

Single-cell multi-omics sequencing is a new technology to accurately analyze cell heterogeneity and gene regulatory network at single-cell resolution, and provides a brand new perspective of pathway analysis on cell differentiation, interaction and heterogeneity. Cardiac regeneration is an important research direction for reversing myocardial injury and remodeling. At present, single-cell omics sequencing technologies have been wildly applied in the field of cardiac regeneration. Great achievements and prospects have been made in the following aspects: landscape of cardiomyocyte proliferation under physiological and pathological conditions; cardiomyocyte differentiation trajectory and interaction of non-cardiomyocytes on cardiac regeneration; new evaluation system for cardiomyocyte proliferation based on consecutively monitoring cell cycle molecular markers; molecular switch regulating cardiomyocyte proliferation. This review summarizes the latest research and progress in the field of cardiac regeneration and discusses the application and development direction of single-cell technologies in the field in order to provide positive and reasonable reference for the future research.

The similar scale model experiment can provide some reference for the research and development of naval gun weapons. This paper takes the naval gun bracket as an example to explain. Based on the dimensional analysis, equation analysis and finite element method, the modal parameter similarity relationship between the original model for bracket and the shrinkage ratio model is established. The results show that the error of predicting the natural frequency of the original model based on similarity relation is less than 2% comparing with the results of finite element numerical simulation, and the error is less than 10% comparing with the experimental results, and the mode of shrinkage model is close to that of the original model. It is proved that the theoretical method in this paper is feasible and practical in engineering. Therefore, the vibration characteristics of the original model can be estimated by analyzing the vibration characteristics of the carrier shrinkage ratio model, which provides a reference for ship gun designers.

The overburden movement law of the upward fully mechanized working face is different from that of the horizontal working face. The roof collapses frequently, which brings a great test to the stability of the working face support. The existing research on the safe mining of the upward fully mechanized working face mainly focuses on the influence of the change of the mining angle on the overburden movement law, and there is a lack of systematic research on the force characteristics of the roof and floor. In order to solve the above problems, taking the 8102 upward fully mechanized working face in Ruilong Mine as the background, the UDEC numerical simulation software is used to analyze the overburden movement law and roof fracture characteristics at different advancing distances of the upward fully mechanized working face. In the upward working face, affected by the inclination angle and mining method, the bottom coal stress is most concentrated, and the working face has obvious characteristics of initial pressure and periodic pressure. Compared with the nearly horizontal fully mechanized working face, the periodic pressure step is obviously reduced, the peak strength of the overburden is relatively low, the roof is not easy to form a structure, the pressure is more frequent, and the mine pressure appears more intense. The step distance of the first collapse of the direct roof of 8102 working face is 25 m, the step distance of the initial pressure of the basic roof is 40 m, and the step distance of the periodic pressure is 10-15 m. The existing calculation methods of using the roof-support mechanical relationship to determine the working resistance of support are relatively cumbersome. Many methods are not practical when applied to the engineering site. By comparing the advantages, disadvantages and practicality of several commonly used support working resistance calculation methods, it is concluded that the dynamic load calculation method is the most suitable method for calculating the working resistance of the support according to the actual working conditions of 8102 working face. The method has the characteristics of accurate calculation results and easy selection of parameters. It is determined that the maximum working resistance of the support of this working face is 6 359 kN/frame, and the support of this working face with a working resistance greater than 7 066 kN can meet the support requirements. The results of engineering application have proved the correctness of using dynamic load calculation method to calculate the working resistance of the support of 8102 working face.

Intracranial hemorrhage (ICH) is one of the most common cerebrovascular disease. At present, there is still a lack of drugs with definite curative effects for the treatment of intracranial hemorrhage. Microglia is a type of immune cell that specifically exists in the central nervous system. In the acute phase of intracranial hemorrhage, microglia is rapidly activated and can dynamically transform between M1 phenotype (pro ⁃ inflammatory) and M2 phenotype (repair). These two different polarization states suggest that microglia plays a complicated role in the pathophysiological process of secondary neuroinflammation after intracranial hemorrhage. Therefore, this is also an important target for exploring drugs for the treatment of intracranial hemorrhage. This article reviews the surface markers of microglia after intracranial hemorrhage, polarization phenotype, phagocytic hematoma, and potential connections with neurons, astrocytes, and oligodendrocytes, in order to find the possibility of treating intracranial hemorrhage by regulating the function of microglia. doi：10.3969/j.issn.1672⁃6731.2021.02.003

Gas drainage by drilling is the main method of gas drainage. Based on this, gas drainage drilling with different diameters has been carried out. The study on the evolution characteristics of the surrounding plastic zone during the process shows that under the influence of drilling disturbance, expansion and deformation occurs around the hole, resulting in plastic zone, elastic zone and original stress zone; at the same time, the distribution of tangential stress around the five boreholes successively experienced a process of sharp increase, slow decrease until stress equilibrium. The larger the hole diameter, the farther the maximum point of stress around the hole is from the center of the hole; and it is found that the larger the hole diameter, the wider the width of the plastic zone. That is to say, the wider the pressure relief range around the borehole, the more adsorbed gas will be resolved into the free state, resulting in the expansion of the gas drainage radius; at the same time, according to the field test, it is found that when the width of the borehole plastic zone increases, the effect of gas extraction is significantly improved with the increase of pressure relief range around the hole.

Recent progress in studies on quantum chromodynamics (QCD) phase transition and related critical phenomena within the functional renormalization group (fRG) approach were reviewed, including the nonperturbative critical exponents and baryon number fluctuations, which are pertinent to the critical end point (CEP) in the QCD phase diagram. The fRG is a nonperturbative continuum field approach, in which quantum thermal fluctuations are successively integrated with the evolution of the renormalization group (RG) scale. Different methods of finding solutions to the flow or fixed-point equations of a nonperturbative effective potential have been discussed, for example, the Taylor expansion, expansion of the spatial dimension ε=4-d, and the recently proposed direct solution of the global potential. Furthermore, the baryon number of fluctuations is relevant to the critical phenomena of the CEP. Both have been discussed, and one explores the underlying reasons for the observed non-monotonic dependence of the kurtosis of the net proton number of distributions on collision energy in experiments.

BackgroundThe corrosion of the secondary circuit has remained a challenging problem influencing the security and efficiency of a nuclear power plant. In an actual operation, an amount of alkalizer is usually added into the secondary circuit water to adjust its pH value to alleviate the corrosion of the pipelines. However, the very complex real working condition results in a significantly inhomogeneous distribution of the pH values, and the enclosed nature of the secondary circuit have frustrated various efforts to control the precise pH values at some key sites inside the circuit. So far, pH values around such key locations have been roughly estimated by either external simulating experiments or by using patented commercial software of foreign companies. However, it is difficult for such external simulations to take into account the various important working conditions.PurposeThis study aims to develop a method without specific assumptions or uncontrolled approximations to calculate the distribution of pH values in the secondary circuit under its working conditions.MethodsFirstly, the complex and repeated structure inside the steam generator was simplified and simulated by using one direct tube model. Except for the length, the other geometrical dimensions, support plates as well as the tube material etc. were chosen to be as similar as possible to the actual ones. Then, all temperature-dependent equilibriums involving H+ in water solution of the secondary circuit were considered with additional consideration of the equilibriums of the alkalizers in the gas and liquid phases. As an essentially field quantity defined on the space of the secondary circuit, these pH values together with other relevant parameters, such as temperature, fluid velocity etc. were depended on the position coordinates, and were calculated by using the finite element method coded in the COMSOL package. Finally, the boundary conditions of flowing rate and temperature of the water at the inlet were set as 1.0 m·s-1 and 543.2 K, respectively, and the pressure at the outlet was set as ~6.8 MPa, a stepwise linear heat flux model was used to simulate the thermal energy transfer from the primary side to the secondary one. The bubbly-flow model was used to simulate the actual steam-water fluid in the secondary side, which was assumed to be in a steady state working condition.ResultsThe calculated pH field under the working conditions shows clearly an inhomogeneous distribution, e.g. ΔpH = ~ -0.6 from z = 0 to 3.8 m, due to the influences of the tube support plates, the temperature and the heat transfer, etc. The investigations on the ammonia/ethanolamine (ETA) binary alkalizer with different total concentrations of various NH3/ETA molar ratios show a better enhancing effect of ETA over ammonia for the pH value (ΔpH>~0.14), and reveal a saturation effect (molar ratio NH3:ETA≤ ~1:4).ConclusionsThe distribution of the pH values in the realistic working conditions can be calculated without resorting to empirical formulae and uncontrolled approximations. The developed method and the calculated results provide valuable information for solving the corrosion problem in the secondary circuit. The method and the model can be extended to simulate the more realistic conditions of a nuclear power plant.

In order to intuitively reflect the evolution process of the cracks on the surface of similar material samples and the location and shape of the cracks, sand, cement, gypsum and other materials are used to make similar material samples, and the non-contact strain measurement system is used to observe and study the uniaxial compression state, the whole process of the destruction of samples of similar materials. The test results show that compared with the traditional test method, the digital image correlation technology has obvious advantages. The full-field strain cloud image obtained by this method can better reflect the evolution characteristics of the cracks in the failure process of the similar material samples; the “X”-shaped deformation concentrated zone on the sample surface is taken to measure points, and the strain curve of each measuring point is analyzed by VIC-3D analysis software, and the deformation characteristics of the deformation concentrated zone on the sample surface are obtained; compared with the axial strain rate, the change characteristics of the surface strain rate of the specimen at each stage are obtained.

In this paper, the monitoring mechanism of the two main faults of contact heat and partial discharge of the mine switchgear is studied, the passive wireless temperature sensor monitoring technology based on SAW(surface acoustic wave) sensing technology and the partial discharge monitoring technology based on detecting impedance are proposed, and the monitoring implementation scheme is designed, the application in Shenhua Xinjiang Energy Company has achieved good results.

This paper analyzes the main causes of underground cable fire and the existing cable fire monitoring technology, puts forward the temperature monitoring technology based on the infrared non-contact type high-voltage cable in coal mines, expounds the working principle of the temperature monitoring technology for the infrared non-contact type cable, and clarifies the characteristics of the technology. The technology has achieved good results in the application of Shenhua Xinjiang Energy Co., Ltd.

To study the effect of tunnel section mutation on the time and temperature of smoke spreading to the air inlet and outlet, Pyrosim fire simulation software was used to establish different slope of the tunnel, length and location of sectional mutation of different tunnel sections. In addition, visibility, temperature and smoke spread measurement points are set at different spatial locations. The simulation results show that, the simulation results show that with the increase of roadway inclination, the inhibition effect of roadway mutation on flue gas spread decreases; the higher the intensity of the fire source, the higher the inhibition rate of the sudden change of roadway on the time of smoke spreading to the air inlet. However, the inhibition rate of the smoke to the air outlet time decreased. When the section length of the tunnel is 10 m and the distance from the fire source is 50 m, the effect of the section mutation on the smoke spread is the greatest.

In order to ensure stable and reliable operation of shaft boring machine, an shaft boring machine control system based on PLC was designed, and composition, working principle and software and hardware design schemes were introduced. The system uses two PLC units to control corresponding oil pump, cutting bar rotating motor and pushing and supporting cylinder according to working mode, and uploads state parameters to local operation platform or remote controlling computer for display. The practical application shows that the system runs well.

To study the buckling of bionics skew plates in the crane’s vein box girder,the dimensionless derivations of bucking balance differential equations for skew plates has been given when it is affected by the longitudinal load in the oblique coordinate,and the dimensionless equation of boundary conditions is also given. The calculation procedures are described in detail when the differential quadrature methods（ DQM） are used to solve the critical buckling load of simply supported skew plates. Finally,the elastic skew plate subjected to uniaxial loading condition is considered as the case study,and the DQM results are compared with the existing results to verify the accuracy of numerical solution procedures. At the same time,the affecting relation between the critical buckling load and structure parameters for the crane is discussed.

The conventional fire assay method to determine high grade gold has a long chemical procedure with many factors influencing the analysis result. Moreover, the ash blowing process for the Pb button volatilizes a large amount of Pb, causing serious pollution to the environment. In this paper, a method is described whereby the 20.0-100.0 g sample was split into small amounts and calcinated. The products of calcination were dissolved by 50% aqua regia and the residues were filtered. All the clean filtrates were loaded into a volumetric flask. An appropriate volume of solution was selected to extract Au with foam enrichment. After ashing the Au bearing foams, the Au was dissolved again by concentrated aqua regia. Then, the Au was measured by Flame Atomic Absorption Spectrometry (FAAS). The detection limit was 0.101 μg/g. Through verifications for a few national first grade standard materials and the analysis samples, the accuracy and precision met the relevant requirements. The sampling weight was from 20.0 g to 100.0 g which effectively improved the sample representativeness and extended the analysis content range for Au by FAAS. This method is also suitable for samples with Au contents from 50.0 μg/g to 550.0 μg/g.

Boolean logic can not describe fuzzy concepts, such as similarity degree. However, the similarity degree of two programs is usually used to determine that whether one program of these two is a variant of the other. To detect malware, a model for analysis of program maliciousness was put forward based on fuzzy reasoning. In this model, malicious characteristics of a program were abstracted using fuzzy pattern recognition firstly. Then the maliciousness of this program was quantitatively analyzed by probability method. Finally, whether the program is malicious was determined approximately using a fuzzy reasoning algorithm. Experimental results show that the prototype based on this model is good at detecting unknown malicious programs and malware variants.

Recent studies have shown that iron metabolism dysregulation and lipid peroxidation-induced ferroptosis, triggered by oxidative stress, play a key role in the development of aortic dissection. Dysregulated iron metabolism leads to excessive production of hydroxyl radicals due to abnormal iron levels and heme metabolism, while lipid peroxidation is linked to system Xc － dysfunction and accumulation of phospholipid hydroperoxides. These factors synergistically disrupt aortic homeostasis and drive ferroptosis in vascular cells, including endothelial and smooth muscle cells. Furthermore, disruptions in ferroptosis-related genes, along with risk factors such as smoking, epigenetic modifications such as protein methylation, and abnormalities in immune cells, particularly T cells, are closely linked to aortic dissection. Several small molecules and nanomaterials have shown potential in inhibiting ferroptosis in this context. This review elucidates the roles of ferroptosis in aortic dissection and proposes strategies for its targeted prevention and treatment.

Objective: To analyze the distribution characteristics of foot deformities and the biomechanical features of the feet among elderly diabetic patients in the community. Methods: A total of 3 148 elderly diabetic patients (6 296 feet) who participated in the diabetic foot screening program in Jinshan District, Shanghai, from March to May 2023 were included, with 1 357 males and 1 791 females, aged (70.7±6.0) years. According to arch deformities, the severity of hallux valgus, and the presence or absence of other toe deformities (hammer foot, mallet foot, and claw foot), the patients were grouped and compared in terms of biomechanical parameters such as plantar pressure, force-bearing area, foot axis angle, footprint length, and load ratio. Results: The the most common foot deformity was the combination of arch deformities, accounting for up to 41.0% (2 581/6 296), it was followed by hallux valgus at 21.8% (1 371/6 296) and other toe deformities at 13.4% (846/6 296). A total of 56.5% (3, 557/6 296) of the patients had at least one type of foot deformity, and 29.0% (1 032/3 557) presented with tow or more types of foot deformities. When grouped according to arch deformities, the patients in the low arch group, mild low arch group, normal arch group, mild high arch group, and high arch group was 343, 1 282, 3 715, 595, and 361 cases, respectively; and there were statistically significant differences in maximum pressure, mean pressure, force-bearing area, foot axis angle, anterior-posterior and lateral offsets of the center of plantar pressure, anterior-posterior foot load ratio, and medial-lateral load ratio among all the groups (all P <0.001). The high arch group exhibited the highest maximum and mean pressures on the plantar surface, with the smallest force-bearing area, while the opposite was true for the low arch group (all P<0.001). There were 4 925, 731, 640 cases in the no hallux valgus group, mild hallux valgus group, and moderate-to-severe hallux valgus group respectively when grouped according to the severity of hallux valgus. The moderate-to-severe hallux valgus group had the lowest medial load ( P =0.002) and the highest lateral load ratio ( P =0.001). Compared to the group without other toe deformities ( n =5 450), the group with other toe deformities ( n =846) exhibited higher maximum plantar pressure [(214.43±112.85) vs (202.88±113.63) kPa, P <0.001], a lower forefoot load ratio (54.35%±13.31% vs 55.94%±12.58%, P =0.001), and a higher hindfoot load ratio (45.65%±13.31% vs 44.06%±12.58%, P =0.001). Conclusions: Among elderly diabetic patients, the incidence rate of foot deformities is as high as 56.5%, with arch deformities being the most common. Foot mechanics analysis indicates that the high arch group has the highest maximum and average plantar pressures, but the smallest force-bearing area, while the opposite is true for the low arch group. Toe deformities can affect the patient's plantar load ratio.

Carotid atherosclerotic plaque is the main cause of ischemic stroke. In recent years, with the continuous innovation of novel imaging technologies, numerous classification standards for carotid plaques provide more powerful evidence for the features of carotid plaques and perioperative vascular assessment, as well as the reference for surgeons in choosing therapeutic decisions. Ultrasound is the preferred non-invasive and convenient screening tool for carotid stenosis. Invasive examinations such as CT angiography and magnetic resonance angiography are suitable for carotid stenosis patients to determine the plaque composition and stability, which can guide surgical decision-making and help to prevent serious cardiovascular and cerebrovascular adverse events. Advances in the treatment of carotid artery stenosis have focused on the improvement and innovation of vascular interventional devices and surgical procedures, including double-layer stents, coated stents and transcarotid artery revascularization. As technology continues to evolve, molecular imaging and more minimally invasive screening as well as therapies will be the way forward.

Tumors pose a significant global health concern and have long been a challenging issue in the medical field. Given its treatment dilemma, it is urgent to explore novel prevention and treatment strategies. Bilirubin, as a natural endogenous antioxidant, has the ability to inhibit the production of free radicals in the body, thereby alleviating the damage caused by oxidative stress to the organism. In recent years, the therapeutic effects of bilirubin in diseases mediated by oxidative stress and metabolic disorders have gradually gained widespread attention, demonstrating its potential therapeutic value for a variety of diseases. With further research, significant progress has also been made in the study of bilirubin in the field of oncology, suggesting its potential important role in the occurrence, development, and treatment of tumors. This article aims to review and summarize the recent advances in the study of bilirubin in the field of oncology, in order to provide new insights and guidance for the future directions of tumor diagnosis, prevention, and treatment.