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The study was to analyze six common agricultural and sideline products in southern China, peanut vines, evaluated pitaya vines, banana leaves, sea rice straw, zea mays straw and corn straw nutritional value. The experiment adopted a single-factor randomized design and sample materials were divided into three groups, and routine nutrient composition determination and simulated rumen in vitro fermentation experiment were carried out. The results showed that peanut vines had the highest CP content (P<0.05), banana leaves had the highest CF content (P<0.05), sea rice straw had the highest crude fiber content (P<0.05), and pitaya vines had the lowest NDF and ADF (P<0.05). The RFV, QI, GI, DE, ME and NEg of pitaya vines were significantly higher than those of other five samples (P<0.05). The highest gas production was obtained from pitaya vines after liquid external fermentation in rumen of Leizhou goats (P<0.05). The content of NH3-N was zea mays straw > pitaya vines > peanut vines > sea rice straw > corn straw > banana leaves. The IVDMD was pitaya vines > zea mays straw > corn straw > sea rice straw > peanut vines > banana leaves. The study indicates that nutritional value, feeding value and in vitro fermentation degradation results of six agricultural by-products are different, and affiliation function integrally assesses nutritional value in order of pitaya vines > peanut vines > zea mays straw > corn straw > sea rice straw > banana leaves. [ABSTRACT FROM AUTHOR]

PURPOSE: To observe the effects of endothelial progenitor cells (EPCs) on bone formation of tissue engineered bone in dogs. METHODS: Bone marrow stromal cells (BMSCs) and EPCs were derived from dog bone marrow and cultured in different medium in vitro. They were seeded on demineralized bone matrix (DBM) to build tissue engineered bone, then the construct was implanted into the fasciae of latissimus dorsi muscle, the degree of bone formation was analyzed with imaging and histological methods at different time points. SPSSI3.0 software package was used for statistical analysis. RESULTS: 4, 8, 12 weeks after operation, X-ray film showed bone mineral density (BMD) in the EPCs group was significantly higher than the control group, and there was significant difference between the two groups(P<0.05); Histological examination revealed that the degree of bone formation in the EPCs group was higher than the control group, the new bone area and blood vessel area between the two groups were significantly different(P<0.05). CONCLUSION: EPCs can promote bone formation and accelerate new bone formation in tissue-engineering bone. Supported by National Natural Science Foundation of China (30872896) and Natural Science Foundation of Shandong Province (Y2008C77). [ABSTRACT FROM AUTHOR]

PURPOSE: To investigate the effect of bone marrow stromal cell sheet on the formation of tissue-engineered bone in dogs. METHODS: Demineralized bone matrix (DBM) were prepared from homologous bone. DBM was constituted with recombination human bone morphogenetic protein-2(rhBMP-2). And bone marrow stromal cells(BMSCs) were isolated from iliac bone of dogs with the method of density gradient centrifugation in vitro. BMSCs induced by osteogenic DMEM at passage 3 were incubated in the temperature -responsive culture dish to form BMSCs cell sheet. BMSCs cell sheet combined with DBM/rhBMP-2/BMSCs was implanted around the vessels of latissimus dorsi muscle in the experimental side, and DBM/rhBMP- 2/BMSCs without BMSCs cell sheet was implanted around the vessels of latissimus dorsi muscle in the control side. 4,8,12 weeks after operation, the ectopic bone formation was investigated by histological observation. The Data were analyzed by two independent samples £--test with SPASS 13.0 software package. RESULTS: Osteogenesis result was experimental side>control side. Newly formed bone tissue of two sides was significantly different (P<0.05). 12 weeks after operation, a large number of lamellar bone and Haversian system formed in the experimental side, with red bone marrow in the bone marrow cavity. Lamellar bone formed in the control side without Haversian system and red bone marrow. CONCLUSION: BMSCs cell sheet could promote the formation of tissue-engineered bone with dense lamellar bone and Haversian system. Supported by National Natural Science Foundation of China (30872896) and Natural Science Foundation of Shandong Province (Y2008C77). [ABSTRACT FROM AUTHOR]

To investigate the growth situation of bone marrow stroma stem cells (BMSCs) cell-sheet and endothelial progenitor cells (EPCs) on demineralized bone matrix (DBM) and explore the effect of DBM/BMSCs cell-sheet and DBM/EPCs complex on the construction of functional and vascularized tissue -engineered bone in mongrel. METHODS: BMSC cell-sheet was prepared with cell-sheet engineering approach, EPCs were isolated from canine bone marrow and DBM was prepared from homologous bone. BMSCs cell-sheet and EPCs were inoculated on DBM respectively and observed by inverted phase contrast microscope and scanning electron microscope. The porosity of DBM was measured. RESULTS: The DBM/BMSCs cell-sheet and DBM/EPCs complex were successfully constructed. The BMSCs cell-sheet and EPCs adhered to DBM well and grew rapidly. CONCLUSIONS: The BMSCs cell-sheet and EPCs have good biocompatibility with DBM. The complex of DBM/ BMSCs cell-sheet, DBM/EPCs could provide good conditions to acquire functional and vascularized tissue-engineered bone. [ABSTRACT FROM AUTHOR]

Objective: To investigate the effect of integrative Chinese and Western medical therapy for radiative pneumonia (RP)., Methods: Eighty patients were randomly assigned to two groups. The treated group (48 patients) was treated by Shenqi Fuzheng Injection (SFI), antibiotics and short-term pulse therapy with high efficient hormone, while the control group (32 patients) was treated only by antibiotic and hormone pulse therapy. Changes of symptoms, signs, quality of life and subsets of T-lymphocytes (CD3+, CD4+, CD8+ and CD4+/CD8+ ratio) were observed before and after 20-day treatment., Results: The chief symptom disappearance time was shorter (P < 0.05), Karnofsky performance score was better (P < 0.05) in the treated group after treatment as compared with those in the control group. Moreover, the immune function was improved in the treated group after treatment (P < 0.01)., Conclusion: SFI combined with antibiotics and short-term pulse therapy with high efficient hormone has a good effect for radiative pneumonia, it could also increase patients' quality of life as well.

Objective: To study MRI changes of vertebral metastasis and their value in differential diagnosis., Methods: MR films of 103 patients with vertebral metastasis confirmed clinically or pathologically were reviewed with all features recorded and analyzed., Results: 338 vertebrae were involved in 103 patients, including 82 in vertebral body only, 3 in appendix only and 253 in both. According to the shape of vertebral body and the characteristic abnormality, 335 vertebrae with body involved were divided into 4 types: Type I (97) with one single focus in the vertebral body, type II (102) with multiple foci with clear margin in the vertebral body, type III (16) with abnormal signal in the whole vertebral body and type IV (120) with abnormal signal in the whole or most part of vertebral body complicated with compression fracture. Among all these lesions, 114 showed concave superior and/or inferior edges and 116 protruding posterior and/or anterior borders. In 256 vertebrae with abnormal appendix, 238 showed abnormal pedicle of vertebral arch involving neighbouring part of vertebral body and 235 showed enlarged pedicle and other parts of the appendix. Soft tissue mass was showed around 133 vertebrae, with the center at the involved vertebrae on sagittal image. 130 pieces of these masses extended cranio-caudally within or a little beyond the width of a vertebral body., Conclusion: Vertebral metastasis is characterized by involving multiple vertebrae. Its diagnosis and differential diagnosis can be made definite in most patients according to the lesions distribution, change in vertebral shape and the characteristics of the soft tissue mass.