Acer plants are important landscape trees around the world wideband most of which have high ornamental value. Hut few of the germplasm resources was utilized, and much problem also existed in these plants' crossbreeding work ,such as confusion of the hybrid varieties, less information of the genetic relation between different cultivars, etc. In this study, we analyzed the genetic relationships among 25 samples of Acer plants by inter-simple sequence repeat USSR) molecular-marked technique, and twelve ISSR primers were screened to assess the genomes of 25 samples of Acer plants. The result showed that a total of 84 DNA bands were amplified and 81 of which (96.43%) were polymorphic. The genetic identities among 25 plants varied from 0.405 to 0.952 with an average of 0.627, indicating that the genetic similarity coefficient between different cullivars or species were relatively different. According to the Nei-Li genetic similarity of 0.578,UPGMA method cluster analysis indicated that these 25 samples were classified into 2 cluster groups, and they were classified into 6 subcluster groups with the genetic similarity of 0.646. The result, showed that the genetic relationship among 18 A. palmatum cultivars were close, and a correlation was found between genetic relationship and phenotype, such as leaf shape and leaf colour. Economic values of Acer plants were concluded as important ornamental trees. In future⪢people should paid more attention to collect and protect the gene resources, introduction and domestication, integrative research and development utilization, and in order to realize rational utilization and detailed research of plant resources. [ABSTRACT FROM AUTHOR]