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The dry Maillard reaction method was used to glycosylate rice bran globulin. On this basis, the noncovalent binding of glycosylated rice bran globulin with two polyphenols was studied. The interaction mechanism between them was characterized and analyzed by fluorescence spectroscopy, infrared spectroscopy, differential scanning calorimetry, etc. The results showed that the micromorphology of rice bran globulin was changed after glycosylation, and the anti-digestibility and antioxidant properties were improved. Quercetin and resveratrol could both cause fluorescence quenching of glycosylated proteins, and resveratrol caused a red shift in the emission peak of glycosylated proteins. In this non-covalent binding, hydrogen bonding/van der Waals forces played a major role. Fluorescence fitting data showed that glycosylated proteins could bind both resveratrol and quercetin simultaneously, with binding sites and constants of 0.014 7 and 0.002 23, respectively. The binding ability was lower than that of binding resveratrol or quercetin molecules separately. The particle sizes of glycosylated proteins and glycosylated protein-polyphenol non-covalent complexes were higher than that of rice bran globulin, and the maximum particle size occurred when glycosylated proteins were bound to two polyphenols simultaneously. In addition, the non-covalent binding of glycosylated proteins with two polyphenols could significantly improve the protein’s thermal stability and antioxidant capacity, and this improvement was situated between the glycosylated proteins binding to resveratrol and quercetin molecules alone. [ABSTRACT FROM AUTHOR]

Copyright of Shipin Kexue/ Food Science is the property of Food Science Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Clinical Nursing in Practice is the property of Journal of Clinical Nursing in Practice (Editorial Board, Shanghai Jiao Tong University Press) and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Shipin Kexue/ Food Science is the property of Food Science Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Food Research & Development is the property of Food Research & Development Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Practical Oncology Journal is the property of Journal of Practical Oncology Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Objective: To investigate the change and clinical significance of adenosine deaminase (ADA) in the serum of patients with autoimmune liver disease. Methods: The total ADA (tADA) and the activity changes of its isoenzyme ADA1 and ADA2 in serum of patients with autoimmune liver disease were detected by enzymatic kit, and the total ADA, ADA1 and ADA2 were analyzed by receiver operating curve (ROC). Active diagnostic value. The Spearman correlation was used to analyze the correlation among the indexes in patients with autoimmune liver disease. Results: Compared with the control group, the activities of tADA and ADA2 were significantly increased in the serum of patients with autoimmune liver disease (P<0.001), and the activity of ADA1 was increased to a certain extent (P=0.035). There were no significant differences in serum tADA, ADA1 and ADA2 activities among patients with different autoimmune liver disease subtypes. ROC analysis showed that serum tADA and ADA2 activities had diagnostic value, but ADA1 activity had no diagnostic value. When the cut-off value of tADA activity was 13.5 U/L, the diagnostic specificity was 93.3%, and the sensitivity was 81.2%. When the cut-off value of serum ADA2 activity was 9.5 U/L, the diagnostic specificity was 85.0%, and the sensitivity was 83.3%. However, ADA1 had no significant diagnostic value, ADA activity was significantly negatively correlated with albumin globulin ratio (r=-0.41, P=0.004), and had a certain degree of positive correlation with globulin level (r=0.34, P=0.018), was weakly positively correlated with ALT (r=0.29, P=0.042), and had no statistically significant correlation with other indicators. Conclusion: Serum tADA and ADA2 activities are significantly elevated in the serum of patients with autoimmune liver disease, and have a certain diagnostic value. [ABSTRACT FROM AUTHOR]

Copyright of Modern Food Science & Technology is the property of Editorial Office of Modern Food Science & Technology and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Practical Oncology Journal is the property of Journal of Practical Oncology Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Practical Oncology Journal is the property of Journal of Practical Oncology Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

In order to explore the nutritional and health value of buckwheat varieties, the variation of crude protein, total flavonoids, protein components and fruit characters of 56 different lines of buckwheat seeds were studied. The results were as follows:(1)Common buckwheat, rice tartary buckwheat and golden tartary buckwheat seeds had crude protein content average of 13.19%, 15.44% and 11.75%, respectively, total flavonoids average of 0.14%, 2.50%, and 2.09%, the average of albumin content of 5.22%, 6.13% and 4.56%, respectively, the average of globulin content of 1.29%, 1.15%, and 0.91%, respectively, the average of gliadin content of 0.42%, 0.58%, and 0.55%, respectively, and the average of glutenin content of 2.66%, 3.36%, and 2.80%, respectively. The protein component content orders of all types of buckwheat were in accordance with albumin > glutenin > globulin > gliadin.(2)Among the fruit traits, the variation coefficient of 1 000-fruit weight, fruit area and diameter of common buckwheat were the largest, and the variation coefficient of fruit perimeter, fruit length-width ratio, fruit length, fruit width and 50 mL volumetric weight of rice tartary buckwheat fruit were the largest.(3)Correlation analysis showed that there are significant or extremely significant correlations between crude protein content and length-width ratio and fruit length in common buckwheat, between crude protein content and fruit perimeter and fruit length in golden buckwheat, between crude protein content and fruit width, between total flavonoids content and fruit area, fruit width, fruit diameter and 50 mL volumetric weight in rice tartary buckwheat fruits.(4)Through the study, the buckwheat lines with high protein content or high flavonoid content, such as common buckwheat Guitian 1808-166, golden tartary buckwheat(Duoku74, Duoku78),black rice buckwheat(1906-136 and 43-2)were found. The results in this study have some guiding significance for the quality breeding of buckwheat and the development of new buckwheat products. [ABSTRACT FROM AUTHOR]

Copyright of Acta Agriculturae Zhejiangensis is the property of Acta Agriculturae Zhejiangensis Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Shipin Kexue/ Food Science is the property of Food Science Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

‘Guiduoku 75' bred by distant hybridization was selected to investigate the major agronomic traits, yield traits and quality traits in ratooning plants under the 40 cm(I), 20 cm(Ⅱ)and 10 cm(Ⅲ)of stubble height treatment. The results indicated that the plant height, shoot weight, grain number per plant, yield per plant and yield in ratooning plants were significantly altered by stubble height when stubble height was less than 40 cm. The values of plant height, shoot weight, grain number per plant, yield per plant and yield in ratooning plants under treatment I were significant or extremely significant higher than those of treatments Ⅱ and Ⅲ, suggesting that higher stubble height is beneficial for vegetative organ growth and yield formation in ratooning plants. ‘Guiduoku 75' seeds contained four protein components as albumine content > gluten content > prolamine content > globulin content. The total protein content and gluten content were significantly altered by stubble height, but the albumin content, prolamine content, globulin content were not when stubble height was less than 40 cm. The total flavonoid content in ratooning plants of ‘Guiduoku 75' ranged from 3.17% to 3.33%, that was little affected by stubble height. The ratooning plants of ‘Guiduoku 75' under 40 cm of stubble height treatment harvested 1.5 t·hm-2 seeds that contained 4.06% of albumin content, 3.26% of gluten content, 1.27% of prolamine content, 0.39% of globulin content and 3.33% of total flavonoid content, exhibiting high nutritional value and medicinal value. [ABSTRACT FROM AUTHOR]

Copyright of Food Research & Development is the property of Food Research & Development Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Food Research & Development is the property of Food Research & Development Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Food Research & Development is the property of Food Research & Development Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of China Brewing is the property of China Brewing Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

【Objective】This paper aimed to carry out the hybrid breeding of Huoshou black pigs and Berkshire pigs and provide a data basis for further breed selection.【Method】Ba-Huo pigs were acquired by means of orthogonal experiments(Berkshire pig♂×Huoshou black pig♀), and then the serum biochemical indexes of 30, 60, 90, 180 and 300 day-old Huoshou black pigs(HS) and Ba-Huo pigs(BH) were measured using fully automatic biochemical analyser, respectively. The developmental differences of serum biochemical indexes between Huoshou black pigs and Ba-Huo pigs were compared and analyzed. 【Result】The content of alanine aminotransferase(ALT), aspertate aminotransferase(AST) in the serum of Huoshou black pig was extremely significant higher than that of Ba-Huo pig at 60, 180 and 300 days(P<0.01), the total protein(TP) was significantly higher than that of Ba-Huo pig at 30 days(P<0.05), and triglyceride(TG) level was significantly higher than that of Ba-Huo pig at 180 days(P<0.01), the high-density lipoprotein cholesterol(HDL-C) and low density lipoprotein cholesterol(LDL-C) were significantly higher than that of Ba-Huo pig at 300 days(P<0.01). The serum contents of alkaline phosphatase(ALP), glucose(GLU) levels of Ba-Huo pigs at 30, 180, and 300 days were extremely significant higher than that of Huoshou black pigs(P<0.01), triglyceride(TG) levels of Ba-Huo pigs at 30, 90, 300 days were extremely significant higher than that of Huoshou black pigs(P<0.01), and total cholesterol(TC) contents were higher than that of Huoshou black pigs at 60, 180, 300 days(P<0.05). The albumin-globulin ratio(A/G) content of Huoshou black pig was higher than that of Ba-Huo pig at 30, 60 and 300 days(P<0.01), but lower than that of Ba-Huo pig at 90 and 180 days(P<0.01). The content of albumin(ALB), globulin(GLB) in serum of Huoshou black pigs were extremely significantly higher than that of Ba-Huo pigs at 90, 180 and 60, 300 days, respectively(P<0.01). 【Conclusion】The results indicated that serum ALT, AST and ALP indexes related to production traits, serum GLU, TC, TG, HDL-C and LDL-C indexes related to fat deposition ability, and serum TP, ALB, GLB and A/G indexes related to the immune ability of Ba-Huo pigs and Huoshou black pigs were significantly different at different days. It was suggested that there might be some differences in growth performance, fat deposition and immunity indexes between Ba-Huo pigs and Huoshou black pigs during the growth and fattening period. [ABSTRACT FROM AUTHOR]

Copyright of Food Research & Development is the property of Food Research & Development Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of China Brewing is the property of China Brewing Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Shipin Kexue/ Food Science is the property of Food Science Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Food Research & Development is the property of Food Research & Development Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

To get insight into globulin genes in Arachis duranensis, genome - wide identification and expression analysis of AdARA gene family were investigated using bioinformatics methods. Results showed that A. duranensis contained 9 globulin genes, physicochemical among the family members were not significant. Motif compose of the closely related proteins were similar. Phylogenetic of globulins revealed that evolutionary relationship of globulins accorded with genetic relationship between species. AdARA proteins were conservative in evolution process and under negative selection, but positively selected sites were identified at a clade. By analyzing RNA - seq transcrip-tome data of cultivar peanut, it showed that 15 arachin genes were expressed in seed, the expression of 4 arachin genes greatly exceeded the others, the expression level of 4 arachin genes were also high at Pattee stage 10. [ABSTRACT FROM AUTHOR]

Copyright of Chinese Journal of Oil Crop Sciences is the property of Oil Crops Research Institute of Chinese Academy of Agricultural Sciences and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Objective: To optimize the extraction process parameter of globulin Brucea javanica seeds, obtain the polypeptide components by enzymatic hydrolysis, and evaluate its cytotoxicity. Methods: The orthogonal experiment of L9 (33) was firstly applied to obtain the optimal parameter for globulin extraction. The protein was then primarily purified by isoelectric precipitation. Different kinds of proteases were applied to hydrolyze B. javanica globulin followed by ultrafiltration with the molecular weight cut-off of 3 kDa to collect the permeate. MTT assay was then conducted to evaluate the cytotoxicity of low molecular weight hydrolysates (S 3kDa) on B16 cell line. Results: The optimal process parameters of the globulins extraction were as follows: 5 % of NaCl concentration, 1.5 h of extraction time and solid liquid ratio of 1:12; The hydrolysates (S 3kDa) of B. javanica globulin produced by pepsin showed significantly high growth inhibitory activity on B16 cells, with the IC50 value of 1.08 p.g -mL"1 after 72 h incubation. Conclusion: The peptides of B. javanica globulin demonstrated specific cytotoxicity on melanoma cell line in vitro, suggesting antitumor peptides could be further obtained from B. javanica seeds. [ABSTRACT FROM AUTHOR]

Objective: To investigate the risk factors and construct a nomogram model for predicting the occurrence of cirrhotic portal vein thrombosis in patients combined with esophagogastric variceal bleeding (EVB). Methods: Clinical data on 416 cirrhotic PVT cases was collected from the First Hospital of Lanzhou University between January 2016 and January 2022. A total of 385 cases were included after excluding 31 cases for retrospective analysis. They were divided into an esophagogastric variceal bleeding group and a non-esophagogastric variceal bleeding group based on the clinical diagnosis. The esophagogastric variceal group was then further divided into an EVB group and a non-bleeding group. All patients underwent gastroscopy, serology, and imaging examinations. The risk factors of PVT combined with EVB were identified by univariate analysis using SPSS 26. The prediction model of cirrhotic PVT in patients combined with EVB was constructed by R 4.0.4. The prediction efficiency and clinical benefits of the model were evaluated by the C-index, area under the receiver operating characteristic curve, calibration plots, and decision curve. The measurement data were examined by a t -test or Mann-Whitney U test. The counting data were tested using the χ (2) test or the Fisher exact probability method. Results: There were statistically significant differences in the etiology, Child-Pugh grade,erythrocyte count, hematocrit, globulin, and serum lipids between the esophageal and non-esophageal varices groups ( P < 0.05). There were statistically significant differences in etiology, erythrocyte count, hemoglobin, hematocrit, neutrophil percentage, total protein, globulin, albumin/globulin, urea, high-density lipoprotein cholesterol, calcium, and neutrophil lymphocyte ratio (NLR) between the EVB and non-bleeding groups ( P < 0.05). Multivariate logistic regression analysis showed that etiology ( OR = 3.287, 95% CI : 1.497 ~ 7.214), hematocrit ( OR = 0.897, 95% CI : 0.853 ~ 0.943), and high-density lipoprotein cholesterol ( OR = 0.229, 95% CI : 0.071 ~ 0.737) were independent risk factors for cirrhotic PVT patients combined with EVB. The constructed normogram model predicted the probability of bleeding in patients. The nomogram model had shown good consistency and differentiation (AUC = 0.820, 95% CI : 0.707 ~ 0.843), as verified by 10-fold cross-validation (C-index = 0.799) and the Hosmer-Lemeshow goodness of fit test ( P = 0.915). The calibration plot and the decision curve suggested that the prediction model had good stability and clinical practicability. Conclusion: The risk factors for EVB occurrence include etiology, erythrocyte, hemoglobin, hematocrit, percentage of neutrophils, total protein, globulin, albumin/globulin, urea, high-density lipoprotein cholesterol, calcium, and NLR in patients with cirrhotic liver. The constructed prediction model has good predictive value, and it can provide a reference for medical personnel to screen patients with high bleeding risk for targeted treatment.

ТÐ3/4 analyze the rationality of delivery method for heраtitis B immune globulin (HBIG ) in раtients with СЫÐÐС heÑ€ati-tis B virus (HBy ) infertion after liver trаnsÑ€1аntаtion аnd to investigаte the guiding Ñ€rinÑiÑ€le for mediÑаtion. Methods Forty - four Ñаses of heраtitis B - related liver trаnsÑ€1аntаtion who раrtiÑiраted in the Ñ€hаse 1У Ñ1iniÑа1 trial of HBIG for intrаvenous injertion from August 2008 to DeÑember 2010 were аnа;yzed. These раtients were divided into severe liver diseаse grouÑ€, liver ÑаnÑer grouÑ€, аnd liver Ñirrhosis grouÑ€. The Ñ€ositive rаtes of HBy DNA аnd HBeAg were Ñomраred between grouÑ€s by СЫ - squаre test. The HBV DNA level wаs Ñomраred by analysis of vаriаnÑe. The Ñorrelаtion between blood ÑonÑentrаtion аnd hаlf - life of HBIG аt different doses аnd dosing intervа1s wаs аnаlyzed by Peаrson's Ñorrelаtion test. The titer of аnti - HBs аt one week аfter oÑ€erаtion wаs Ñomраred between HBeAg - negаtive grouÑ€ аnd HBeAg -Ñ€ositive grouÑ€ аnd between high - HBy DNA grouÑ€ аnd low - HBy DNA grouÑ€ by t test. Cаses of reinfeÑtion were аlso monitored. Results There were no signifiant differenÑes in the Ñ€ositive rates of HBy DNA аnd HBeAg between the severe liver diseаse grouÑ€, liver ÑаnÑer grouÑ€, аnd liver Ñirrhosis grouÑ€ (x² = 4.871, P=0.088; x²=1.079, P=0.583). No signifiant diferenes in meаn HBy DNA level were found between these grouÑ€s (F=0.895, P=0.418) . The Pearson's Ñorrelation analysis revealed а signifiÑant negative Ñorrelation between the blood ÑonÑentration and half-life of HBIG (r=0.988, P=0.012). The tier of anti - HBs showed no signifiant diferenes between HBeAg-negative grouÑ€ and HBeAg-Ñ€ositive grouÑ€ and between high-HBy DNA grouÑ€ and low-HBy DNA grouÑ€ one week after oÑ€eration (t=1.757, P=0.087). No Ñases of reinfeÑtion were found during the observation Ñ€eriod. Conclusion It is neÑessary to give HBIG 4000 IU during oÑ€eration and 2000 IU dai1y for 6 days after oÑ€eration, and the dose should be inÑreased aррroÑ€riately when the раtients have relatively high viral load in serum. For the Ñ€atients whose serologiÑa1 markers of heÑ€atitis B beÑome negative, the antibody level can be maintained at about 100 IU/L until the next administration if 600 IU of HBIG is given once by a month by intramuscular injection. [ABSTRACT FROM AUTHOR]

Using a Japanese variety RiB with ( α' + α ) - subunits deficiency of 7S globulin as the donor parent, and Dongnong 47 with higher protein and oil content as recurring parent, characteristic of &$945; - subunit deficiency was successfully induced into Dongnong 47 by the methods, which combined with SSR marker assistance and values of agronomic and quality traits based on crossing and backcrossing. A new elite α - null - containing mutant line ( Cb80), showing greater than 90% recurring parent genome content, with higher protein and oil content had been developed in BC2F4. The protein and total amino acids contents of Cb80 were 44.1% and 41.95%, which were higher than the recurring parent Dongnong 47 whose ratios were increased by 3.5 % and 3.76% respectively. [ABSTRACT FROM AUTHOR]

To investigate the application of bromocresol green Colorimetry (BCG) method in measuring serum albumin (ALB) and to evaluate its influencing factors in different diseases. This study was a cross-sectional study that included 128 people admitted to the department of nephrology, department of general surgery, department of infectious diseases and other departments of the Third Xiangya Hospital of Central South University in July 2021. They were divided into groups according to disease types, including chronic kidney disease group (47 cases), liver disease group (40 cases), other diseases group (41 cases), serum ALB was detected by BCG method and immunoturbidimetry at the same time, and the results were expressed as ALB BCG and ALB I respectively, each group was subdivided into three subgroups according to ALB I results: relatively high-value subgroup, relatively intermediate-value subgroup and relatively low-value subgroup of albumin. ALB I and ALB BCG were compared in all groups and subgroups. Passing-Bablok regression and Bland-Altman diagram analysis were used to evaluate the application of ALB BCG in each group. Immunoturbidimetry was used as a reference method to evaluate the bias of ALB BCG , and the differences between ALB I and ALB BCG were shown as follows: Δ ALB= ALB BCG -ALB I . Pearson correlation analysis and multiple linear regression analysis were used to assess the correlation between Δ ALB and ALB autoconcentration (ALB I ), α 1 -globulin, α 2 -globulin, β 1 -globulin, β 2 -globulin, γ-globulin, creatinine (Cr), urea (UN), uric acid (UA), aspartate aminotransferase (AST), alanine aminotransferase (ALT), total bilirubin (TBil), direct bilirubin (DBil), and C-reactive protein (CRP) levels.The results showed that ALB BCG were higher than ALB I in the relative low subgroups of total patients group, chronic kidney disease group, liver disease group and other disease groups, and the differences were statistically significant ( t value was 8.025, 6.878, 2.628, 4.915, respectively, P <0.05). In the relatively high value subgroup, ALB BCG was lower than ALB I , and the differences were statistically significant in the relative high value subgroup of total patients group, liver disease group and other disease groups ( t value was -4.388, -2.927, -3.979, P <0.05). Passing-Bablok regression and Bland-Altman analysis showed that the BCG method had proportional bias. In the chronic kidney disease group, the concentrations of ALB I and Cr had the greatest influence on BCG bias, and the regression model equation was Δ ALB=5.437-0.146× Alb I -0.001 ×Cr, R ²=0.505. In the liver disease group, the concentrations of ALB I , α 1 -globulin, β 1 -globulin had the greatest influence on BCG bias, and the regression model equation was Δ ALB=3.652-0.230×ALB I +0.398×α 1 -globulin+1.171×β 1 -globulin, R ²=0.658. In the other disease group, the concentration of ALB I and α 2 -globulin had the greatest influence on BCG bias, and the regression equation was Δ ALB=5.558-0.225×Alb I -0.281×α 2 -globulin, R ²=0.646. The BCG method has a proportion error, and its bias may lead to unacceptable differences. BCG method is mainly affected by the concentration of ALB itself, and may also be affected by α 1 -globulin, α 2 -globulin, β 1 -globulin, Cr.

Objective: To investigate the prognostic value of albumin/globulin ratio on postoperative survival outcomes in patients with hepatocellular carcinoma. Methods: Data of 630 patients with HCC, who underwent surgical resection from February 2009 to July 2013, were retrospectively analyzed. Patients were divided into low-value group (A/G < 1.5, defined as L group) and high-value group (A/G≥1.5, defined as H group), and their distribution characteristics were observed with the normal A/G threshold value. Independent risk factors' affecting survival and prognosis was analyzed with univariate and multivariate Cox's regression model. Survival trend of all patients with low-value and high-value groups in A, B and C of Barcelona stage (BCLC stage) were analyzed using the Kaplan-Meier method. Results: Multivariate analysis showed that preoperative A/G ratio ( P = 0.007), alpha-fetoprotein ( P < 0.001), gamma-glutamyltransferase ( P = 0.006), RBC ( P = 0.014), international normalized ratio ( P = 0.009), preoperative BCLC staging ( P < 0.001) and number of tumors ( P = 0.003), and intraoperative blood transfusion ( P < 0.001) were independent prognostic factors affecting long-term survival in HCC patients. The median overall survival time in-group L was 15 months, significantly lower than that in group H of 42 months ( P < 0.001). Stratified analysis showed that the short-term survival advantage of patients with high A / G value was limited to those with Barcelona stage A ( P < 0.001), and disappeared in patients with Barcelona stage B and C ( P > 0.05). The long-term survival advantage existed in patients with Barcelona stage A ( P < 0.001), B ( P < 0.05), and disappeared in C ( P > 0.05). Conclusion: Preoperative albumin/globulin ratio can predict postoperative prognosis and survival, and direct towards the treatment for early stage of HCC and thus representing as an indicator of high clinical value.

In order to find the insect-resistant composition and differentially expressed proteins of mungbean, Jinlv No.7, B20 and Weilv 2117 were used as experimental materials, and the differential proteins and functions of mungbean varieties that are resistant and susceptible to bruchids were compared and analyzed by two dimensional gel electrophoresis (2-DE) and identified by mass spectrometry. Among the samples, 15 protein spots showed a more than 2.5 times reproducible up-regulated, significance 6 of them were successfully identified by the database, and involved three kinds of proteins. They are the alpha and beta subtype 8S globulin, ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBis CO) subunits binding protein and the precursor peptide chains for amylase inhibitor and trypsin inhibitor. The protein expressions of B49 (alpha subtype 8S globulin) and B31 (RuBis CO subunits binding protein) of insect-resistant mungbean were 10 000 and 23 times higher than that of insect-susceptible mungbeans. It stunted the growth and even death of the Callosobruchus chinensis L. that alpha and beta subtype 8S globulin and RuBis CO subunits binding protein and precursor peptide chains for amylase inhibitor and trypsin inhibitor of insect-resistant mungbean, the bruchid resistance effect of these three proteins need to further verified in terms of the quantity and the combined effect.

Objective: To investigate the diagnostic value of insulin like growth factor I(IGF-I), β2-microglobulin (β2MG) and serum ferritin (SF) in patients with multiple myeloma (MM) and their ralationship with clinical staging., Methods: Seventy-seven patients with MM treated in Depertment of Hematology of Shanghai 10th hospital and Oncology of Shanghai Armed Police Hospital from August 2016 to June 2017 were enrolled in MM group, at same period 77 healthy volunteers were enrolled in normal control group. The diagnostic value of IGF-I, β2-MG and SF for MM, and their levels in different stages of MM were compared., Results: The ROC analysis showed that β2-MG and SF alone as well as their combination had the diagnostic significance for MM, moreover the diagnostic value of IGF-I, β2-MG and SF combination was highest, but the single IGF-I did not possess diagnostic significance for MM. The comparison of IGF-I, β2-MG and SF levels in different stages of MM showed that the β2-MG and SF levels in I stage were higher than those in normal control group (P<0.05), but lower than those in II and III stages (P<0.05). The IGF-I level in I stage was not statistically and significantly different from IGF-I level in normal control group (P>0.05), but lower than those in II and III stage (P<0.05). The relationship analysis between IGF-I and β2-MG, SF in different stages showed that the IGF-I related with SF in I stage (r=0.417), but did not relate with β2-MG; the IGF-I in II stage related with β2-MG and SF in II stage (r=0.543, r=0.426); IGF-I related with β2-MG and SF in III stage (r=0.425 and r=0.672)., Conclusion: The diagnostic value of IGF-I, β2-MG and SF alone does not high for MM, but their combination can significantly enhance the occurate rate of MM diagnosis. The levels of IGF-I, β2-MG and SF in II and III stages of MM all increase, moreover the level of IGF-I correlates with the levels of β2-MG and SF.

Objective: To analyze the genotype-phenotype correlations among southern Chinese Han prenatal population in Guangdong area with δ-globin gene mutation, so as to enrich the delta-thalassemia gene mutations data. Methods: A total of 33 cases were selected in 7 580 patients during prenatal thalassemia trait screening, from January 2012 to May 2015(including 10 males and 23 females, aged 22-48 years old). Complete blood cell count was performed on a XE 4000i automatic hemocyte analyzer. Hb, HbF and HbA2 were tested by high performance liquid chromatography (HPLC). Genomic DNA was extracted from whole blood samples using a whole blood genomic DNA extraction kit. Polymerase chain reaction (PCR) was used to amplify three different fragments corresponding to the exons and the regulatory sequences using three different couples of primers for the δ-globin gene. Results: Twenty one of the 33 samples were positive for the δ-globin gene defects. Four previously known mutations were detected: including 12 cases for -77(T>C)[HBD c. -127 (T>C)](57.14%), 4 cases for -30 (T>C)[HBD c. -80 (T>C)](19.05%), 1 case for codon 10 (-G) (HBD c. 31delG)(4.76%), and 1 case for HBD c. 244 C>T(4.76%). Three new δ-globin gene defects which had not yet been reported in database were detected, including 1 case for HBD c. 22&#95;24delGAG(4.76%), 1 case for HBD c. 347 C>T(4.76%), and one case for HBD c. 349 C>G(4.76%). Conclusions: -77 (T>C) is the most common mutation in Chinese southern prenatal population. Three new HBD gene mutations are referred in this report, which provide the valuable information for genetic counseling and prenatal diagnosis in Guangdong area.

Objective: We detected and analyzed the clinical values of total protein (TP), albumin (ALB), globulin (GLB), and ALB/GLB ratio (A/G) of whole unstimulated saliva of healthy people to determine the time of day when saliva composition is relatively stable. We compared the protein concentration and A/G of whole unstimulated saliva of patients with chronic periodontitis with those of healthy volunteers to provide references for diagnostic methods and clinical applications of saliva., Methods: The whole saliva of 37 healthy subjects were collected at 8:00, 9:30, 11:30, 13:00, 16:30, and 21:00. Meanwhile, the whole saliva of 24 patients with periodontitis was collected in the morning. Bicinchoninic acid method was used to detect the TP content. Saliva ALB was detected by GF-D800 semi-automatic biochemical analyzer, and the GLB and A/G were calculated. Finally, the results were statistically analyzed using SPSS 19.0., Results: Salivary protein compositions were stable in the morning on an empty stomach. Healthy people: TP, (1 354.35±389.52) µg.mL-1; ALB, (139.55±27.19) µg.mL-1; GLB, (1 211.80±360.73) µg.mL-1; A/G, 0.126 3±0.041 7. Subjects with chronic periodontitis: TP, (2 611.56±231.62) µg.mL-1; ALB, (296.27±17.34) µg.mL-1; GLB, (2 315.69±221.67) µg.mL-1; A/G, 0.156 2±0.017 3. The contents of TP, ALB, and GLB in healthy individuals at different periods within a day showed significant differences (P<0.05), which were mainly reflected in the levels before and after meals. No significant difference was detected in A/G. The concentrations of TP, ALB, and GLB were significantly increased in patients with chronic periodontitis compared with those in healthy volunteers. However, no significant difference existed in A/G., Conclusion: Salivary protein compositions are more stable in the morning than in other periods. Thus, mornings can be set as the time of specimen collection in future research. The concentrations of TP, ALB, and GLB in patients with chronic periodontitis are higher than those in healthy people.

Following the sequential Osborne extraction procedure, the proteins of lotus seeds were classified. The secondary structures of albumin, globulin, prolamine and glutelin fractions were determined by Fourier transform infrared spectroscopy (FTIR). The FTIR images of amide I and III bands from the four protein fractions were analyzed using Fourier deconvolution and curve-fitting technique. The results showed that there were minor differences in every corresponding peak position and peak area percent of secondary structure between albumin and globulin as well as between prolamin and glutelin. But there were differences in every corresponding peak position between albumin (or globulin) and prolamin (or glutelin). Especially the area percents of the corresponding nonrandom structures (alpha-helix and beta-sheet) of albumin and globulin were significantly larger than those of prolamin and glutelin. The contents of nonrandom structures of albumin and globulin extracted with 0.1 mol x L(-1) NaCl solution were about 55% and those of prolamine and glutelin fractions were only at round 40%, indicating that the secondary structures of the salt-extraction protein were ordered and stable.