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Objective： This study aimed to improve the preservation properties of pullulan （PUL） coating and prepare preservation materials with sustained-release properties. Methods： GSE nanoparticles were prepared by grape seed extract （GSE）, chitosan and γ-polyglutamic acid. GSE nanoparticles of different quality were added to the PUL coating solution to prepare GSE nanoparticles/PUL coatings. The preservation properties of GSE nanoparticles/PUL coatings on salmon fillets were evaluated. Results： Compared with untreated fish fillets, the total number of bacteria in fish meat was reduced, and lipid oxidation and protein decomposition were delayed by 1.08% GSE nanoparticles/PUL coating. In addition, after fish fillets were treated with 1.08% GSE nanoparticles/PUR coating, the protein solubility was increased by 38.8%, and the activities of superoxide dismutase and glutathione peroxidase were increased by 4.02 U/mg and 0.20 U/g, respectively. Conclusion： The GSE nanoparticles/PUL coatings were prepared with good preservation properties, which can extend the shelf life of salmon fillets for 5~7 days.

Aiming at the efficiency and credibility of data feed in power blockchain application system,the trusted data feed technology based on 5G and Oracle mechanism is studied. Firstly,the data feed method in the blockchain system is discussed,and the Oracle data feed method applicable to the power 5G blockchain is analyzed. Secondly,the trusted data feed technology of the power 5G business system based on distributed Oracle is proposed,and the overall system architecture based on cloud-edge-terminal integration is designed,as well as the workflow of data source registration,evaluation and on chaining. Among them,the distributed data collection and sharing are realized through the blockchain system,and the data source evaluation is realized based on threshold signature algorithm and verifiable random function. The evaluation algorithm is run by the edge Internet of Things (IoT) agent node to ensure the security and availability of the system. Through the division and optimization of 5G slice tasks,the efficient transmission of authentication data and business data is achieved. Finally,the proposed scheme is deployed in the electricity 5G blockchain based power information acquisition system,and experimental verification is carried out from the aspects of communication performance,business performance,resource utilization,etc. The results show that the average data transmission delay of the proposed scheme under different load stress tests is about 10 ms,and the bit error rate and packet loss rate are less than 0.9%. In the case of 100-level concurrent service requests,the performance of the scheme is improved by more than 80% compared with the existing schemes,so it has good feasibility and promotion value.

In recent years, new orthopaedic implantable devices continue to emerge, which require higher requirements for technical evaluation. Animal study is an important part of the research and development process for the new orthopedic implantable devices, which provides relevant evidence for product design and stereotyping. By introducing the purpose of animal study, and the application of 3R principle (replacement, reduction, refinement) in this field, we summarize the concern on the animal study, in order to provide reference for the development and research of new orthopedic implantable devices and biomaterials. At the same time, the application of evidence-based research methods such as systematic review in the field is introduced, which provides new tools and approaches for the technical review and regulatory science.

With the development of additive manufacturing, the advantages of this type implant devices in the treatment of acetabular defects and reconstruction are becoming more and more prominent. The number of registration and declaration of such products is increasing day by day. According to the relevant requirements of the National Medical Products Administration for registration and application documents, combined with the characteristics of acetabular reconstruction implant products made of additive manufacturing, this study analyzes and summarizes the relevant requirements on raw material control, product performance research, product manufacturing, clinical evaluation, et al. We should pay more attention to in the registration and application materials submitted by the applicant. Provide opinions and suggestions for the next registration applicant to standardize product R&D and registration application documents, in order to help them optimize product R&D process, improve product quality and registration application efficiency.