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[Objective] The level and coordination of spatial and temporal characteristics of land ecological security and rural revitalization and development in Guangxi Zhuang Autonomous Region from 2010 to 2020 were studied in order to promote land ecological security protection and to improve the level of rural revitalization and development, resulting in coordinated development of both factors. [Methods] We analyzed data from 14 cities in the Guangxi Zhuang Autonomous Region to build an index system. The comprehensive measurement level and coupling coordination model were used for analysis. [Results] ① The level of land ecological security increased over time. ② The overall development level of rural revitalization initially declined and then increased, but the development level of each city was different. ③ The coordination degree changed in the form of “uneven rise and fall”. The level of coordination degree was distributed in a trend of gradual increase from east and west to the central region, from inland to the coast, and from the central region to the north. The development pattern was “low from east to west, high from south to north”. ④ Different development modes and development centers should be adopted according to the regional types of the 14 cities. [Conclusion] Coordinated development of land ecological security and rural revitalization in the Guangxi Zhuang Autonomous Region has not been stable. In the future, we should strengthen the integrated development of land ecological security and rural revitalization in low-level coordination areas, and combine them with policies, people’s livelihood, and development promotion measures to improve the coordination level between the two factors. At the same time, we should stabilize the coordinated development between the two factors in mature areas to prevent loss of existing coordinated development.

Background and purpose: Long non-coding RNA (lncRNA) has been found to be dysregulated in breast cancer and is closely related to the malignant behavior of tumors. This study aimed to explore the effects of LINC02163 targeting miR-338-3p on the proliferation, invasion and migration of breast cancer cells. Methods: Breast cancer tissue samples and the adjacent tissue samples (2 cm to cancer tissue) were collected from 9 female breast cancer patients admitted to People’s Hospital of Zhengzhou from January 2020 to September 2021. Real-time fluorescence quantitative polymerase chain reaction (RTFQ-PCR) was used to detect the expression of LINC02163 in tissue samples, human normal breast epithelial cell line (MCF-10A) and breast cancer cell lines (MCF-7, BT-20, MDA-MB-231, T47D). MDA-MB-231 was divided into control group, sh-NC group, sh-LINC02163 group, sh-LINC02163+inhibitor-NC group and sh-LINC02163+miR-338-3p inhibitor group. MTT method, transwell test and scratch test were used to detect the MDA-MB-231 cell viability, invasion and migration ability. Western blot was used to detect the protein expression of c-Myc, matrix metalloproteinase (MMP) 2, MMP9, E-cadherin and N-cadherin in MDA-MB-231 cells. Dual luciferase reporter gene was used to detect the targeting relationship between LINC02163 and miR-338-3p. In vivo tumor formation experiment was used to detect tumor volume and weight in nude mice. Immunohistochemical method was used to detect the Ki-67 proliferation index in tumor tissues of BALB/c nude mice. Results: Compared with adjacent tissues, the expression of LINC02163 in breast cancer tissues was significantly higher (P0.05), the cell survival rate, number of invasive cells, migration rate, expressions of c-Myc, MMP2, MMP9 and N-cadherin, tumor volume and weight, and Ki-67 proliferation index were significantly increased, while the expressions of miR-338-3p and E-cadherin were significantly reduced (P

Objective: Citron Rho-interacting serine/threonine kinase (CIT) was identified recently as an oncogene involved in the progression of various malignant tumors, but its role in prostate cancer (PCa) remains unclear. In this study, we aimed to investigate the biological functions of CIT in PCa., Methods: We analyzed the expression of CIT in PCa tissues and its clinical correlations based on the Cancer Genome Atlas (TCGA) and Memorial Sloan-Kettering Cancer Center (MSKCC) dataset. We then examined the effects of RNA interference-mediated CIT silencing on the proliferation, migration and invasion of PC-3 cells using cell counting kit-8, wound healing assay and Transwell assay. We also investigated the effect of CIT silencing on epithelial-mesenchymal transition (EMT) and Hippo-Yap signaling pathway in the cells using Western blotting., Results: CIT expression was significantly elevated in PCa tissues from TCGA cohort ( P < 0.05). MSKCC dataset analysis showed that an elevated expression of CIT was significantly correlated with N stage ( P =0.001), distant metastasis ( P < 0.001), Gleason score ( P =0.010) and PSA ( P =0.004). In cultured PC-3 cells, knockdown of CIT significantly inhibited cell proliferation, migration and invasion, reversed the EMT phenotype and decreased the expression and activity of YAP., Conclusions: CIT might function as an oncogene in PCa by modulating the Hippo-YAP signaling pathway and serve as a candidate therapeutic target for PCa.