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Copyright of Acta Edulis Fungi is the property of Acta Edulis Fungi and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Zhejiang University (Science Edition) is the property of Journal of Zhejiang University (Science Edition) Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

To reveal the genome genetics and evolution and clarify the systematic relationship and the formation of modern geographic distribution, a karyotype analysis of mitotic metaphase chromosomes in 51 Epimedium taxa(43 species, 1 subspecies, 6 varieties, and 1 cultivar)and two Vancouveria species was conducted in the present study. Especially, the 53 taxa studied were clustered by their karyotype resemblance-near coefficients because the interspecific karyotypes are very similar and the traditional karyotype analysis cannot provide significant evidence for the studies on genetics and evolution in Epimedium. The results were as follows: The 53 taxa studied all were diploid with 12 chromosomes(2n=2x=12), the genome of each taxon had one pair of satellites located on the pair I of homologous chromosomes, respectively, and the karyotype types of the 53 species studied all were type 1A or 2A of Stebbins. It can be concluded that the interspecific karyotypes were indeed very similar and the genomes evolvement were rather conservative in genus Epimedium. The cluster analysis of karyotype resemblance-near coefficients can provide some valuable clues for the studies on the systematics and taxonomy in genus Epimedium. Results of the cluster analysis by the karyotype resemblance-near coefficients strongly supported the previous taxonomic division of Subgen. Rhizophyllum and Subgen. Epimedium in genus Epimedium. The results also showed that the interspecific relationship was closely correlated to the geographical distribution in genus Epimedium and that the genomes of the taxa native to East Asia had obvious variation and more high genetic diversity. Finally, the formation of modern geographical distribution of genus Epimedium was inferred in the present study. Results of the present study have significant scientific values in the further studies on the resources utilization, taxonomy, and phylogenetics in genus Epimedium. [ABSTRACT FROM AUTHOR]

Objective: To study the effects of total glucosides of paeony (TGP) on the expression and apoptosis of endoplasmic reticulum stress factor CCAAT/ enhancer binding protein homologous protein (CHOP), glucose regulated protein 78 (GRP78), glucose regulated protein 94 (GRP94) in rats with myocardial ischemia reperfusion (I/R) injury. Metiiods: 75 health clean SD rats were selected and divided into 5 groups according to the digital random list method, with 15 rats in each group, which were recorded as sham operation group, I/R group, 50 mg/kg TGP group, 100 mg/kg TGP group and 200 mg/kg TGP group. The levels of CHOP, GRP78, GRP94 in the rats of each group were detected and compared. The indexes of myocardial I/R, the infarct area rate and the apoptosis rate of myocardial cells of each group were compared and analyzed. Results: The levels of CHOP, GRP78 and GRP94 in the I/R group and TGP group were significantly higher than those in the sham operation group, and those were significantly lower in the I/R group than each group of TGP (all P<0.05). The levels of CHOP, GRP78 and GRP94 in 100 mg/kg group and 200 mg/kg TGP group were significantly lower than those in 50 mg/kg TGP group, and those were significantly lower in the 200 mg/kg TGP group than in the 100 mg/kg TGP group (all P<0.05). In the I/R group and each group of TGP, the T-wave changes in 30 min after ischemia, T-wave changes in 120 min after reperfusion and LVEDP level were higher than those in the sham operated group, while the levels of LVSP, +dp/dtmax and -dp/dtmax were significantly lower than those in the sham operated group (all P<0.05). Compared with the I/R group, the T-wave changes in 30 min after ischemia, T-wave changes in 120 min after reperfusion and LVEDP level decreased in a dose-dependent manner, while the levels of LVSP, +dp/dtmax and -dp/dtmax increased in a dose-dependent manner (all P<0.05). The infarct size and myocardial apoptosis rate in I/R group and each group ofTGP were significantly higher than those in sham operated group(all P<0.05). Compared with the I/R group, the infarct size and myocardial apoptosis rate of the TGP group decreased in a dose-dependent manner (all P<0.05). Conclusion: TGP can significantly reduce the expression of endoplasmic reticulum stress factors CHOP, GRP78 and GRP94 in MIRI rats, and can adjust the indexes or the level of related markers of myocardial ischemia and reperfusion,and also can effectively reduce the infarct area rate and myocardial cell apoptosis rate. [ABSTRACT FROM AUTHOR]