1. [Production of shikonin by cell cultures of Lithospermum erythrorhizon].
- Author
-
Hu L
- Subjects
- Cell Culture Techniques methods, Cells, Cultured metabolism, Culture Media, Indoleacetic Acids metabolism, Indoleacetic Acids pharmacology, Lithospermum physiology, Phenylalanine metabolism, Phenylalanine pharmacology, Plants, Medicinal cytology, Time Factors, Ventilation, Lithospermum cytology, Lithospermum metabolism, Naphthoquinones metabolism, Plants, Medicinal metabolism
- Abstract
Objective: To explore cultural conditions of shikonin production by cell cultures of Lithospermum erythrorhizon., Method: Orthogonal design was applied in determination of shikonin within the medium. Flask test was applied in the study of shikonin production by the amount of ventilation., Result: The best medium consisted of 100 mg/L L-phenylalanine, 2 mg/L IAA and 800 mg/L Ca(NO3)2 4H2O. The best amount of ventilation was get by shaken at 150 r/min., Conclusion: This test provided data for producing shikonin by cell cultures of Lithospermum erythrorhizon.
- Published
- 2004