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Objective To investigate the effects of Hes1 gene on the proliferation, migration and invasion of hepatocellular carcinoma cell line Hep1-6. Methods Cell proliferation, cell colony formation, migration and invasion were measured after Hes1 gene were overexpressed in Hep1-6 cells mediated by recombinant adenovirus. Results The clone numbers of Hep1-6 cells infected with Ad-Hes1 were significantly less than the control group infected with Ad-GFP (P < 0.001). Six days after Ad-Hes1 infection, OD450nm of Hep1-6 cells were significantly lower than that of control group (P < 0.01). The wound healing rates of Hep1-6 cells infected with Ad-Hes1 at 24h and 48h were significantly lower than those of the control group (P < 0.001). The number of Hep1-6 cells infected with Ad-Hes1 migrating into the lower chamber of Transwell after 48 h of culture was significantly lower than that of the control group (P < 0.001). The number of Hep1-6 cells infected with Ad-Hes1 invading into the lower chamber of Transwell after 48h of culture was significantly lower than that in the control group (P < 0.01). Conclusion Hes1 could suppress the proliferation, migration and invasion of Hep1-6 cells.

Insulin is produced and secreted by pancreatic β cells in the pancreas, which plays a key role in maintaining euglycemia. Insufficient secretion or deficient usage of insulin is the main cause of diabetes mellitus (DM). Drug therapy and islets transplantation are classical treatments for DM. Pancreatic β cell replacement therapy could help patients to get rid of drugs and alleviate the problem of lacking in transplantable donors. Pancreatic β-like cells can be acquired by cell reprogramming techniques or directed induction of stem cell differentiation. These cells are proved to be functional both in vitro and in vivo . Some hospitals have already performed clinical trials for pancreatic β cell replacement therapy. Functional pancreatic β-like cells, which obtained from in vitro pathway, could be a reliable source of cell therapy for treating DM. In this review, the approaches of obtaining pancreatic β cells are summarized and the remaining problems are discussed. Some thoughts are provided for further acquisition and application of pancreatic β cells.

Based on the analysis of the theoretical calculation model of axial resolution of optical coherence tomograph for the posterior segment of the human eye, a set of testing device for measuring its axial resolution is designed and developed. In view of a commercial ophthalmic optical coherence tomograph in clinical use, its axial resolution is calculated to be 5.07 μm theoretically, and the actual measurement value is 5.45 μm. The uncertainty of the detection device is evaluated and the result is (5.45±0.10) μm. The measurement error introduced by the testing device is very small. Meanwhile, the axial resolution measured by the testing device meets the requirements of the instrument(≤ 6 μm).

Based on the Gullstrand I model eye, a simplified model eye for testing fundus imaging device is designed. The model eye can reach the following requirements:(1) The refractive characteristics of the ocular refractive tissue are simulated, and the equivalent focal length in air is 17 mm; (2) The differences between relative refractive index differences of the adjacent materials of the simplified model eye and relative refractive index differences of any adjacent two layers (cornea and aqueous humor, aqueous humor and lens, lens and vitreous body) of the Gullstrand I model eye are not more than 1%; (3) In the case of the incident aperture diameter of 3 mm, the differences of radii of the diffuse spots formed by the paraxial light and the axial light are not more than 15%; (4) The differences of angles of chief ray and tangent line of the fundus are not more than 1°; (5) In the case of the incident aperture diameter of 3 mm, the differences of MTF values of the near axis light are not more than 0.1. The simplified model eye can be expected to be used for testing fundus imaging device instead of the test method in ISO 10940:2009 Ophthalmic instruments-Fundus cameras.

Different from the previous knowledge regarding reactive oxygen species (ROS), recent research suggests that ROS play essential roles in initiating cascade reaction of wound healing. During wound healing, ROS can serve as the second messenger to regulate signal transduction and gene expression. In this paper, we review the mechanism of generation of cyclooxygenase 2-prostaglandin E2 induced by ROS, which regulates the early inflammatory response and subsequent healing after injury.

Objective: To test the possibility of using spermatogonial cells as vector in the establishment of HBV X gene transgenic mice., Methods: Eukaryoti expression vector pcDNA3-X of hepatitis B virus X gene was constructed. The recombinant pcDNA3-X packed by liposome was injected into testicle tissue of male C57BL/6N mice using microvolume injector. Six weeks after the first injection, the mice were crossed with female mice. Polymerase chain reaction and immunohistochemistry were applied to identify the viral DNA(HBx gene) harbored n liver tissue., Results: The sequence of the X gene is consistent with that reported in literature. The X gene can be expressed in Hela cells. The positive rate in daughter mice was 6.25%., Conclusion: It is possible to use spermatogonial cells as vector to establish HBx transgenic mice.