1. Adenovirus-mediated Hes1 Expression Inhibits Proliferation and Migration of Hepatocellular Carcinoma Cells
- Author
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YU Bing, YIN Gang, and HU Yiping
- Subjects
hes1 ,adenovirus vector ,hcc ,proliferation ,migration ,invasion ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
Objective To investigate the effects of Hes1 gene on the proliferation, migration and invasion of hepatocellular carcinoma cell line Hep1-6. Methods Cell proliferation, cell colony formation, migration and invasion were measured after Hes1 gene were overexpressed in Hep1-6 cells mediated by recombinant adenovirus. Results The clone numbers of Hep1-6 cells infected with Ad-Hes1 were significantly less than the control group infected with Ad-GFP (P < 0.001). Six days after Ad-Hes1 infection, OD450nm of Hep1-6 cells were significantly lower than that of control group (P < 0.01). The wound healing rates of Hep1-6 cells infected with Ad-Hes1 at 24h and 48h were significantly lower than those of the control group (P < 0.001). The number of Hep1-6 cells infected with Ad-Hes1 migrating into the lower chamber of Transwell after 48 h of culture was significantly lower than that of the control group (P < 0.001). The number of Hep1-6 cells infected with Ad-Hes1 invading into the lower chamber of Transwell after 48h of culture was significantly lower than that in the control group (P < 0.01). Conclusion Hes1 could suppress the proliferation, migration and invasion of Hep1-6 cells.
- Published
- 2020
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