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Objective:To explore the levels of miR-454-3p and STAT3 in nasopharyngeal carcinoma (NPC) tissues, as well as the effect of miR-454-3p targeting STAT3 on the process of epithelial-mesenchymal transition (EMT) in NPC cells. Methods:NPC tissue and healthy tissue were collected in five cases from the Affiliated Hospital of Youjiang Medical University for Nationalities and Baise People's Hospital from June to August 2023. qRT-PCR detected the expression of miR-454-3p and STAT3 in each tissue sample. Untreated NPC cells CNE-2 was used as a blank control group (Blank group) . miR-454-3p mimics, mimics NC (negative control), miR-454-3p inhibitor and inhibitor NC (negative control) were transfected into CNE-2 cells by LipofectamineTM 3000 as miR-454-3p mimics group, mimics NC group, miR-454-3p inhibitor group and inhibitor NC group. The targeting relationship of miR-454-3p and STAT3 was predicted by Targetscan and confirmed by the dual-luciferase assay. The transfection efficiency and STAT3 mRNA levels were assessed using qRT-PCR following transfection. Wound healing and transwell experiments were executed to assess cell migratory and invasive abilities. The expression levels of STAT3 and proteins associated with EMT were assessed using Western blot analysis. Results:The expression of miR-454-3p was reduced in NPC tissues relative to healthy tissues, while the expression of STAT3 was elevated (all P<0.001) . The dual-luciferase assay findings indicated that miR-454-3p targeting regulates expression of STAT3 (P<0.001) . Compared to both the blank group and mimics NC group, STAT3, N-cadherin, and Vimentin expressions of the miR-454-3p mimics group were reduced. Conversely, it exhibited an increase in E-cadherin expression. The migratory and invasive capabilities of the cells were weakened (all P<0.05) . Compared to the blank group and inhibitor NC group, the expressions of STAT3, N-cadherin, and Vimentin in the miR-454-3p inhibitor group were upregulated. In contrast, the expression of E-cadherin was downregulated. Cells showed both enhanced migratory and invasive capacity (all P<0.05) . Conclusion:In NPC tissues, miR-454-3p demonstrated downregulation, whereas STAT3 exhibited upregulation. Notably, within CNE-2 human NPC cells, miR-454-3p significantly reduced STAT3 activity and effectively suppressed cell invasion and migration. [ABSTRACT FROM AUTHOR]

Objective To investigate expression changes of phospholipase C-γ1 (PLCγ1)/inositol triphosphate receptor (IP3R)/calmodulin-dependent kinase Ⅱ (CaMKⅡ) and density of dendritic spine in the hippocampus of autism model mice induced by sodium valproate (VPA). Methods Pregnant C57 mouse was given intraperitoneal injection of 300 mg/kg VPA or same volume of normal saline at the 10th and 12th days of gestation. The offspring exposed to VPA or saline during gestation were identified as autism spectrum disorder mice (VPA group) or normal control mice (CON group). Western blotting was used to detect the expression levels of PLCγ1, IP3R, CaMKⅡ and post-synaptic dense-95 (PSD95) in the hippocampus of the 2 groups. Golgi staining was employed to observe the changes in dendritic spines in the hippocampus of the offspring mice. Results Western blotting showed that the expression levels of PLCγ1, IP3R, CaMKⅡ and PSD95 were decreased in the VPA group than the normal control group (P < 0.01). Golgi staining indicated that compared with the normal control group, the density of dendritic spines in the hippocampus of VPA mice was reduced (P < 0.01). Conclusion In the hippocampus of VPA induced autism mice, the expression of PLCγ1/IP3R/CaMKⅡ and PSD95 and the dendritic spine density of pyramidal neurons in hippocampal CA1 are all decreased compared with the normal control mice.

Objective: To explore the relationship between blood lipid indicators and different clinical classifications of dyslipidemia and diabetic kidney disease (DKD) and to compare the value of different clinical classifications of dyslipidemia for predicting DKD., Methods: Continuously enrollment of subjects was conducted at the First Affiliated Hospital of Chongqing Medical University and the Yongchuan Hospital of Chongqing Medical University between October 2020 and October 2021. A total of 356 type 2 diabetes mellitus (T2DM) patients admitted to the two hospitals were enrolled. They were divided into DKD group ( n =126) and simple T2DM group ( n =230) according to whether their T2DM was combined with DKD. In addition, 250 healthy individuals undergoing physical examination during the same period were enrolled for the control group. The blood pressure, blood lipid, blood glucose, and the kidney function indicators of the three groups were measured. The effects of different classifications of dyslipidemia on DKD were analyzed with unconditional logistic regression models, the receiver operating characteristic (ROC) curve was constructed, the area under the curve ( AUC ) of ROC was calculated, and the value of different classifications of dyslipidemia for predicting DKD was analyzed., Results: The diastolic blood pressure (DBP), systolic blood pressure (SBP), total cholesterol (TC), triacylglycerol (TG), low-density lipoprotein cholesterol (LDL-C), serum creatinine (Scr), uric acid (UA), and glycosylated hemoglobin A1c (HbA1c) of the DKD group and the simple T2DM group were significantly higher than those of the control group, while the high-density lipoprotein cholesterol (HDL-C) levels of the DKD group and the simple T2DM group were lower than that of the control group (all P <0.05). The disease course of T2DM, DBP, SBP, TC, TG, Scr, UA and HbA1c of the DKD group were significantly higher than those of the T2DM group (all P <0.05). After adjusting for the effects of T2DM disease course, DBP, SBP, Scr, UA and HbA1c, the results showed that TC ( OR =1.426, 95% CI : 1.088-1.868) and TG ( OR =1.404, 95% CI : 1.075-1.833) were independent risk factors for DKD, and that hypercholesterolemia ( OR =1.817, 95% CI : 1.040-3.177) and mixed hyperlipidemia ( OR =2.148, 95% CI : 1.110-4.159) were independent risk factors for DKD (all P <0.05). The AUC (95% CI ) of hypercholesterolemia was 0.789 (0.729-0.871). The AUC (95% CI ) of mixed hyperlipidemia was 0.671 (0.579-0.760). Hypercholesterolemia showed better predictive value for the diagnosis and prediction of DKD., Conclusion: Among the blood lipid indicators, TC and TG are independent risk factors of DKD. In the clinical classifications of dyslipidemia, hypercholesterolemia and mixed hyperlipidemia are independent risk factors of DKD. Hypercholesterolemia can be used as a predictor to screen for DKD among T2DM patients and is well suited for extensive application in outpatient screening., (Copyright© by Editorial Board of Journal of Sichuan University (Medical Sciences).)

Background: To evaluate therapeutic outcome of limited stage small cell lung cancer treated with chemotherapy alone or combined with radiation therapy with different doses., Methods: A retrospective analysis was performed in 128 limited-stage small cell lung cancer patients who were treated with three different ways of treatment, from February 1988 to March 1998 in Heilongjiang Cancer Hospital. All patients were pathologically proved. Forty-two patients received chemotherapy alone (C), 48 patients were treated by interdigitating chemoradiotherapy (IDG) and other 38 patients received concurrent chemoradiotherapy (CON). For thoracic radiation, 20 patients received a dose of ≤45 Gy, 23 ≥60 Gy, and 43 > 45 Gy but < 60 Gy ., Results: The 3-year survival rates were 23.7%, 20.8% and 4.8% in the CON, the IDG and the C groups respectively. There was a significant difference between the CON and the C groups ( P < 0.05), as well as between the IDG and the C groups ( P < 0.05). There was no remarkable difference between the CON and the IDG groups ( P > 0.05). Loco-regional recurrence rate was significantly higher in ≤45 Gy group (55.0%) than that in ≥60 Gy group (8.7%)( P < 0.01)., Conclusions: The chemotherapy combined radiotherapy may improve the survival of patients with limited-stage small cell lung cancer. Dose of thoracic radiation might be related to the loco-regional recurrence.