1. Effects of ING5 gene on the malignant phenotype of breast cancer Bcap-37 cells
- Author
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Yang SONG, Yi-zeng WAN, Shu-peng ZHAO, Feng-jie QI, Lei FANG, Ji-cheng WU, Shuai SHI, and Hua-chuan ZHENG
- Subjects
lcsh:R5-920 ,inhibitor of growth ,Bcap-37 cells ,lcsh:R ,breast neoplasms ,lcsh:Medicine ,genes, tumor suppressor ,lcsh:Medicine (General) - Abstract
Objective To investigate the effects of inhibitor of growth 5 (ING5) gene on the proliferation, apoptosis, migration and cell cycle of human breast cancer Bcap-37 cells. Methods The eukaryotic ING5-expressing plasmid and GFP-empty plasmid were steadily transfected in Bcap-37 cells, the expression of green fluorescent protein was measured with fluorescence microscopy, and the high expression of ING5 was measured by real time-PCR. Bcap-37-ING5 cells served as the experimental group, Bcap-37-GFP cells as the mock group and Bcap-37 as the control group. The effects of ING5 on the proliferation were detected by MTT, the cell cycle and apoptosis were detected by Flow cytometry, and the cell migration was detected by cell wound scratch assay and Transwell experiment. Results Bcap-37 cell lines steadily expressing ING5 protein with GFP-tag were acquired by stable transfection. ING5 over-expression inhibited the proliferation and led to G2 arrest of Bcap-37 cells, increased cells apoptosis and decreased the cell migration ability (P
- Published
- 2017