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A series of micro-nanofiber composite membranes with stimuli-responsive properties were prepared using electrospinning technique by combining fluorescent small molecules TPECNPy-2 and TPECNPy-3 with polylactic acid (PLA). The effects of the content of TPECNPy-2 and TPECNPy-3 on the structure and acidochromic properties of the fiber composite membranes were analyzed. The results show that the relative ratio of TPECNPy-2 and TPECNPy-3 has no significant effect on the chemical structure, fiber morphology and aggregation structure of the composite membrane, and the diameter of the prepared fibers ranges between 400~500 nm. Under the action of acid vapor, the maximum fluorescence emission wavelength of the composite fiber membrane change from 492~499 nm before acid fumigation to 542~587 nm. The greater the content of TPECNPy-2 in the system, the more obvious the spectral red shift. The fluorescence emission of the composite membrane after acid fumigation is restored to the initial state by ammonia fumigation. When fluorescent small molecules are added, the contact angle of the fiber membrane increaseds from 101.6° to 125°. The series of composite fiber membranes have good reversible acidochromic properties. The hydrophobicity of the fiber membrane is significantly improved after the addition of two organic small molecules. [ABSTRACT FROM AUTHOR]

In order to resolve the existed problems when analyzing large and complex network systems, a novel attack graph construction method is proposed which is based on distributed parallel processing technology. Firstly, from the defender's point of view, all the vulnerable hosts are considered as attack targets, using positive, breadth-first search strategy to construct attack graph, which resolves the problem of which the attack target is defined and single in the existed methods. Secondly, the optimization technology is researched, and the total network is divided into different areas, through multi-engine parallel processing technology, to meet the distribution scalability requirements, the problem of existed methods with high complexity and low scalability is resolved, and which is difficult for large-scale complex network. Finally, the optimization strategy, limited number of attack steps is used, which resolves the existing state explosion problem when constructing the attack graph. Experimental results show that this method can improve the efficiency of attack graph's generation, and reduce the system resource consumption greatly, and it has value for assessing the security of large-scale complex network. [ABSTRACT FROM AUTHOR]

Penile vascular endothelial damage, vasomotor dysfunction, and blood flow deficiency are the major causes of erectile dysfunction (ED). Current management of ED mostly depends on selective phosphodiesterase type 5- (PDE5) inhibitors, which fail for some ED patients. For Peyronie's disease-induced ED, surgical and physical therapies are used in addition to PDE5I medication, but frequently it is difficult to achieve satisfactory results. Recent studies show that the low-intensity extracorporeal shockwave therapy can promote angiogenesis and improve blood flow to the penis, which promises to be a novel effective therapy for ED and Peyronie's disease.

Objective: To establish HPLC fingerprint of Semen Cassiae., Methods: Analysis on a Phenomenex C18 (4.6 mm x 250 mm, 5 microm) column eluted with phases containing acetonitrile and 0.1% phosphoric acid in water in gradient mode. The flow rate was 0.8 mL/min and the detection wavelength was 280 nm. The temperature of column was 30 degrees C. Data of 12 batches of Semen Cassiae, ultramicro powder and powder particle from different sources were analysed by "similarity evaluation for chromatographic Fingerprint of Traditional Chinese Medicine" software, and the similarity of them was compared., Results: Twenty-seven common peaks were identified in the chromatograms,and all the common peaks were separated effectively. Fingerprint of ultramicro powder and Semen Cassiae was basic similar, but fingerprint of powder particle and Semen Cassiae was obviously different., Conclusion: The method is precise, repeatabe and stable. It can be used to identity and evaluate the quality of Semen Cassiae, and provide important theoretical foundation for clinical applications of Semen Cassiae.

Objective: To establish HPLC fingerprint of flavonoids and phenols of Dendrobium nobile., Methods: Phenomenex prodigy ODS(3) C18 column (250 mm x 4.6 mm, 5 microm) was used with a mixture of acetonitrile-0.1% acetic acid as the mobile phase in a gradient mode, the column temperature was 25 degrees C, the flow rate was 1.0 mL/min, and the detection wavelength was 254 nm., Results: The flavonoids and phenols of Dendrobium nobile were well separated, and 10 fingerprint peaks in common were confirmed., Conclusion: This method is simple, accurate with good reproducibility, and can be used specifically for the quality control of Dendrobium nobile.

Objective: To determine the contents of monotropein in the different processed Products of Radix Morindae., Conditions: Kromasil C18 (150 mm x 4. 6 mm,5 micorm) column; The mobile phase was methol-0.4% phosphate solution (5:95-->28.8: 71.2, 15 min); The velocity of flow was 1 ml/min; The detection wavelength was at 210 nm; And column temperature was 25 degrees C RESULTS: There were obvious differences between the crude and kinds of different processed products of monotropein in the Radix Morindae, The contents of monotropein from the different processed products (crude drugs, removed from wood, processed with salt, prepared) were 13.92 mg/g,9.10 mg/g, 9.21 mg/g, 12.86 mg/g, respectively., Conclusion: The different processing methods can make the contents of monotropein in the Radix Morindae.

Objective: To evaluate the effects of Caulis Sinomenii and sinomenine on conditioned place preference (CPP) induced by morphine and brain histamine level in mice., Methods: Sixty mice were randomized into 6 equal groups and morphine (Mor) was injected subcutaneously (9 mg/kg) for 6 consecutive days to induce CPP using a shuttle box. Since the 4th day of training, the mice in 5 of the groups were treated for 3 consecutive days with Caulis Sinomenii (10 g/kg), sinomenine (60 mg/kg), diphenhydramine (30 mg/kg), CP48/80 (5 mg/kg) and L-histidine (750 mg/kg) in addition to morphine (9 mg/kg) treatment, respectively, leaving the other group with exclusive morphine treatment. Another 10 mice received saline injection to serve as saline control group. The content of histamine (HA) in the mouse brain was measured by fluorospectrophotometry., Results: In morphine group, the mice showed significantly extended stay in morphine-paired compartment whose HA content in the brain was markedly increased (P<0.01). Treatment with Caulis Sinomenii and sinomenine resulted in significantly reduced time of stay in morphine-paired compartment and brain HA level (P<0.01)., Conclusion: CPP induced by morphine in mice is associated with increased HA level in the brain. Caulis Sinomenii and sinomenine can suppress the acquisition of place preference induced by morphine and modulate HA level in the central nervous system in morphine-dependent mice.

Objective: To explore the genetic diversity of different farm races of Morinda officinalis on molecular level., Methods: The molecular biological technique-random amplified polymorphic DNA (RAPD) were used., Results: Of the 40-mer arbitary primers, 14 were found to amplify polymorphic products. 3-5 polymorphic bands were amplified by each polymorphic primer on the average. Using UPGMA method all the tested accesions can be clustered into two groups: 4 accessions of Daye were classified as one group, 1 accession of Xiaoye be another group., Conclusion: There actually existed much genetic diversity on molecular level among the different farm races of Morinda officinalis.

Objective: To observe microvessel density(MVD), epithelial stromal vascular cuffing(VC) and expression of vascular endothelial growth factor(VEGF) in human cervical carcinomas and to clarify their significance in the invasion and metastasis of cervical carcinoma., Methods: VEGF and CD34 were stained immunohistochemically (SP) in 57 cases of cervical carcinoma (30 cases of squamous cell carcinoma, 20 of adenocarcinoma 7 of glandular and squamous cell carcinoma), 29 cases of cervical intraepithelial neoplasia (CIN) and 16 cases of normal cervices, meanwhile, MVD and VC were also assayed., Results: There were significant differences among the above 5 groups for MVD P<0.01 . The VC pattern showed a significant difference between cervical carcinoma and CIN or control group P<0.01). The positive rates of VEGF in normal cervical epithelium, CIN, squamous cell carcinoma, adenocarcinoma, glandular and squamous cell carcinoma were 18.8% 3/16, 82.8% 24/29), 93.3% 28/30), 100% 20/20 and 7/7(100%), respectively. There were significant differences between these cervical lesion groups and the control group(P<0.001). The MVD showed significant differences between the positive pelvic node metastasis and negative pelvic node metastasis P<0.05). There was no significant correlation between the expression of VEGF and the tumor diameter, clinical stage, pathologic grade and pelvic node metastasis., Conclusion: The expression of VEGF may play an important role in the angiogenesis of cervical carcinoma. Degree of malignancy of cervical carcinoma has a close association with microvessel density.