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With rapid development and maturity of single-cell measurements, single-cell biology and pathology has become an emerging discipline to understand the disease. However, it is important to address concerns raised by clinicians as to how to apply single-cell measurements for clinical practice, translate the signals of single-cell systems biology into determination of clinical phenotype, and predict patient response to therapies. This paper proposes a new system coined as the clinical artificial intelligent single-cell (caiSC) with the dynamic generator of clinical single-cell informatics, artificial intelligent analyzers, molecular multimodal reference boxes, clinical inputs and outputs, and AI-based computerization. This system provides reliable and rapid information for impacting clinical diagnoses, monitoring, and prediction of the disease at the single-cell level. The caiSC represents an important step and milestone to translate the single-cell measurement into clinical application, assist clinicians’ decision-making, and improve the quality of medical services. There is increasing evidence to support the possibility of the caiSC proposal, since the corresponding biotechnologies associated with caiSCs are rapidly developed. Therefore, we call the special attention and efforts from various scientists and clinicians on the caiSCs and believe that the appearance of the caiSCs can shed light on the future of clinical molecular medicine.

Lonicerae Flos is a Chinese medicinal material and medical-food homologous resource. It has a variety of pharmacological and health functions such as anti-oxidation, immune regulation, anti-inflammation and antibacterial. Based on the introduction of research process in the history of application, functional components and pharmacological activity of Lonicerae Flos resources, this review summarizes the current situation of comprehensive utilization of Lonicerae Flos and its by-product, and deeply discusses the comprehensive utilization of high-value of these resources from the perspectives of drugs, food, cosmetics, veterinary medicine feed and agricultural inputs. Finally, the comprehensive utilization of the Lonicerae Flos resources and the sustainable development of the industry were prospected.

Objective To investigate the change of metabotropic glutamate receptor 5 (mGluR5) expression level in rats with late-stage (the 7th day) traumatic brain injury (TBI) and the role of taurine. Methods The left cerebral TBI rat models were made by using lateral fluid percussion method. A total of 30 specific pathogen free (SPF) male Sprague-Dawley (SD) rats were randomly divided into 3 groups: sham operation group (control group), TBI model group (TBI group) and taurine treatment group (taurine group). Wet and dry weight method was used to measure the brain water content. Real-time fluorescent quantitative polymerase chain reaction (PCR) and Western blotting were used to detect the change of mRNA and protein expression of aquaporin 4 (AQP4) and mGluR5 in each group. Results Compared with control group, the brain water content (t = 4.893, P = 0.002), AQP4 mRNA (t = 6.523, P = 0.000) and protein (t = 4.366, P = 0.008) expression were upregulated, while mGluR5 mRNA (t = 5.776, P = 0.001) and protein (t = 3.945, P = 0.014) expression were downregulated in TBI group. After taurine treatment, the brain water content (t = 2.151, P = 0.140), AQP4 mRNA (t = 1.144，P = 0.432) and protein (t = 0.367, P = 0.804) decreased to normal, while mGluR5 mRNA (t = 1.824, P = 0.216) and protein (t = 1.185, P = 0.414) increased to normal. Correlation analysis showed brain water content was negatively correlated with mGluR5 mRNA (r = -0.617, P = 0.014) and mGluR5 protein (r = -0.665, P = 0.007), while it was positively correlated with AQP4 protein (r = 0.658, P = 0.008). Conclusions Taurine can significantly increase the mGluR5 expression level of brain issue in the late-stage (the 7th day) of TBI and decline brain edema and brain water content. It may be a potential protective agent as an inhibitory neurotransmitter. DOI: 10.3969/j.issn.1672-6731.2016.08.008

Based on the somewhat homomorphic scheme of Kristin Lauter et al.a new technique,called Relinearization with key switching was presented.Combining this technique with modulus switching,a (leveled) fully homomorphic encryption scheme without bootstrapping from RLWE were designed.Homomorphic operations of this scheme is simple,and trivial gate operation given in the scheme can make level structure of circuit clearer.Finally,bootstrapping was used as optimization to evaluate capability of the proposed scheme.

GmGBP1 is a gene related to photoperiod flowering time of soybean. In this experiment, GmGBP1 gene was used as the target, and plant tissue culture was carried out by soybean cotyledonary node transformation method to obtain T2 GmGBPl interference transgenic soybean. The transcript was sequenced to obtain alternative splicing data. The alternative splicing genes in the sequencing results of WT and GmGBP1 -- i interference soybean leaves were further verified by RT - PCR. A total of 3 genes were alternatively spliced, indicating that the decrease of GmGBP1 interference expression led to the alternative splicing of downstream genes, which could affect the flowering time of soybean. This research provided a basis for further study of soybean GmGBPl gene, and laid a theoretical basis for the mechanism of soybean flowering induction. [ABSTRACT FROM AUTHOR]

The Bohai Rim Plain is an important grain-producing area in China. The cultivated land resources have great potential for production, but there are many influencing factors. Understanding the spatiotemporal change characteristics and driving factors of the net primary productivity （NPP） of cultivated land vegetation in this region is of great significance to improve the regional cultivated land production conditions, excavate and enhance the production capacity of cultivated land, and ensure national food security. In this study, Theil-Sen Median trend analysis, Mann-Kendall significance test, Hurst index, and other methods were used to explore the spatiotemporal change characteristics, stability, and sustainability of regional cultivated land NPP. The influence of driving factors on the spatial heterogeneity of cultivated land NPP was analyzed by using optical parameters-based geographical detectors. The results showed that： ① From 2001 to 2019, due to the expansion of construction land during industrialization and urbanization, the cumulative decrease in the area of cultivated land in the Shandong area around the Bohai Sea was 2 004.51 km 2 . ② During the selected research period, the interannual variation of average cultivated land NPP showed a fluctuating and increasing trend. In terms of spatial distribution, the NPP of cultivated land was bounded by the Dongying District, with the spatial heterogeneity in the north being significantly lower than that in the south. ③ The area with increasing NPP of cultivated land accounted for 88.06% of the total area of cultivated land, mainly with low and medium fluctuations. The NPP of cultivated land will maintain an overall sustained trend of increase across the region in the future. ④ The average annual relative humidity study area had the strongest explanatory power for the spatial variability of NPP in cropland, with a q -value of 0.26, followed by surface soil salinity and subsoil salinity, with q -values greater than 0.2. The interactions between the different drivers all showed either nonlinear enhancement or bifactorial enhancement. The results of this study will help to reveal the characteristics of the changes in cultivated land production capacity and its driving forces in the Bohai Sea region and also provide a theoretical basis for the ecological protection and sustainable development of the region.

Quantitative analysis of the spatial non-stationary characteristics of soil salinization influencing factors and the prediction of its spatial distribution are of great significance for the rational use of coastal saline soil resources and the formulation of local prevention and control measures. In this study, the Hekou District of Dongying City, Shandong Province, was used as the study area, and the descriptive statistics of soil salinization status were conducted using classical statistical methods. Spatial autocorrelation theory was used to explore the characteristics of global and local spatial structure of soil salinization in the study area. Influential factors related to soil salinity were selected, and multivariate linear regression （MLR）, geographically weighted regression （GWR）, and multi-scale geographically weighted regression （MGWR） methods were used to model and predict the spatial distribution of soil salinity in the study area and to analyze the spatial heterogeneity of the effects of different influencing factors on soil salinity. The results showed that： ① The mean value of soil salinity in the study area was 5.84 g·kg -1 , indicating severe salinization, with a global Moran's I index of 0.19 （ P <0.00） and obvious spatial aggregation characteristics. ② Among the three models, the MGWR model had the highest modeling accuracy. Compared with that of the MLR model, the R adj 2 of GWR and MGWR improved by 0.05 and 0.07, respectively, and the RSS decreased by 210.13 and 179.95, respectively. ③ The results of MGWR regression showed that the spatial distribution of soil salinity appeared to be mainly affected by the middle soil salinity, soil clay content, and vegetation cover from the mean values of standardized regression coefficients of different influencing factors. Different influencing factors had significant spatial non-stationary characteristics on soil salinization. ④ The results of the spatial distribution prediction of soil salinity in MGWR showed that the areas of high soil salinity （≥6 g·kg -1 ） were mainly distributed in the northern part of the study area, with an overall spatial trend of decreasing from the coast to the interior. The results of the study can be used as a reference for the analysis and predictive mapping of factors affecting soil salinization in the county and on a larger scale using MGWR.

A 660 MW unit of an ultra-low emission coal-fired power plant in the Beijing-Tianjin-Hebei area was chosen for this study. The particulate matter was sampled with a Dekati low-pressure impactor (DPLI) at the inlet and outlet of flue gas cleaning devices including selective catalytic reduction (SCR), low-low temperature economizer (LLTe), electrostatic precipitator (ESP), wet flue gas desulfurization (WFGD), and wet electrostatic precipitator (WESP). A filter sampling system was also used at the inlet and outlet of the WFGD and WESP. The removal efficiencies of PM 1 , PM 1-2.5 , and PM 2.5-10 from different flue gas cleaning devices were obtained after ultra-low emission modification. The results show that SCR increases the mass concentration of fine particulates and PM 1 by 52.11%. The LLTe improves the removal efficiency of the ESP, especially for particles with a range of 0.1-1 μm. The high-efficiency WFGD removes both SO 2 and particulates, but it increases PM 1 . The mass concentration of PM 1 increases by 59.41% and the water-soluble Mg 2+ , Cl - , and SO 4 2- in PM 10 increases. The WESP has a high removal efficiency with respect to PM 1 , PM 1-2.5 , and PM 2.5-10 and can further reduce the dust concentration. Based on an ultra-low emission reform, the final PM 10 emission of this 660 MW unit is 2.04 mg·m -3 .

Objective: To investigate the enhancement pattern of thyroid carcinoma with contrast-enhanced ultrasound (CEUS) for the diagnostic value., Methods: Thirty-one cases with thirty-five thyroid Occupied were retrospectively reviewed. The final diagnosis was confirmed by biopsy pathology. Contrast agent SonoVue and contrast pulse sequencing (CPS) technique were used in this study., Results: Thirty-five thyroid carcinoma presented three enhancement patterns with CEUS. Type I: twenty-three lesions enhanced in a pattern of ring with centripetal fill-in, however, the central part of no contrast agent filling. Type II: five lesions enhanced regularly and homogeneously. Type III: seven lesions enhanced irregularly and homogeneously. Thyroid benign and malignant lesions perfusion time curve, compared to papillary thyroid carcinoma with nodular goiter in the AT, TTP and WT indicators P < 0.05; papillary thyroid carcinoma and thyroid adenoma, compared to WT alone an indicator of P < 0.05., Conclusions: Identification of the different enhancement patterns of thyroid carcinoma could improve the diagnostic ability of CEUS.

Objective: To explore the value of contrast-enhanced ultrasound (CEUS) in the diagnosis of thyroid occupied lesions with injection of sulphur hexafluoride microbubbles., Methods: Fifty nine cases of conventional ultrasonic diagnosis of thyroid lesions in patients with 73 lesions re-sulfur hexafluoride microbubble ultrasound contrast real-time inspection, the use of CEUS and contrast pulse sequencing (CPS)., Results: Seventy-three lesions were satisfied with the dynamic contrast perfusion imaging. Ultrasound contrast prompted the 15 lesions (13 cases) of malignant lesions by postoperative pathology confirmed papillary thyroid carcinoma, ultrasound images showed a low or mixed echo, boundary ambiguity, accompanied by Microcalcification foci, blood flow distribution I rank or grade III. Ultrasound prompted 58 lesions (46 cases) of benign lesions, blood flow distribution of grade II or III, the performance of a variety of two-dimensional ultrasound image, showing cystic, solid or liquid-solid mass, border clearance. Contrast with the surrounding thyroid tissue, lesionsor=20 mm papillary thyroid carcinoma, manifested prior to the beginning of the adjacent thyroid substance to enhance and dissection, showing the form of high-enhanced perfusion; thyroid follicular adenoma as early as the beginning of the adjacent thyroid substance to enhance and dissection, mass retained within the contrast agent longer time, showing the form of high-enhanced perfusion; nodular goiter enhanced for thyroid nodules and adjacent synchronization started to pick up in real terms, after the 6 lesions showed nodular contrast slightly earlier than the adjacent thyroid substance started to pick up, 28 lesions with synchronous real beginning of the adjacent thyroid dissection, 11 lesions of nodular dissipated early on (started receding time < or = 25 s) adjacent to thyroid substance., Conclusion: The real-time CEUS with injecting sulphur hexafluoride microbubbles is valuable in the diagnosis and differential diagnosis of the thyroid occupied lesions.