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Objective: To observe the clinical efficacy of "Tong Guan Li Qiao"(unblocking gates and orifices) needling method combined with swallowing training in the treatment of post-stroke dysphagia (PSD) and its effect on surface electromyography (sEMG) and cerebral microcirculation., Methods: A total of 116 patients with PSD were randomly divided into observation group ( n =59) and control group ( n =57). Patients in the control group received swallowing training on the basis of conventional treatment, whereas those in the observation group additionally received "Tong Guan Li Qiao" needling treatment, both groups were treated for 4 weeks. Swallowing function was assessed by video fluoroscopic swallowing study (VFSS) and standar-dized swallowing assessment (SSA) scale, the clinical efficacy was calculated. The sEMG was detected by a full-featured electromyography, the average EMG (AEMG), integrated EMG (IEMG) and peak value were calculated. Cerebral blood flow peak systolic velocity (PSV), end-diastolic velocity (EDV), mean velocity (MV) and pulse index (PI) were detected by cerebrovascular color Doppler ultrasound., Results: The total effective rate of the observation group was 94.92% (56/59), higher than 82.46% (47/57) of the control group( P <0.05). After treatment, the initial scores, the scores of drinking 5 mL and 60 mL of water in the two groups were significantly lower than those before treatment ( P <0.05), and the scores of drinking 5 mL and 60 mL of water in the observation group were lower than those in the control group ( P <0.05). Compared with the same group before treatment， the AEMG, peak value， IEMG were higher ( P <0.05)， PSV, EDV and MV were faster ( P <0.05) while PI was lower( P <0.05) in the two groups after treatment. Compared with the control group, the AEMG, peak value， IEMG were higher ( P <0.05)， PSV, EDV and MV were faster ( P <0.05) while PI was lower ( P <0.05) in the observation group., Conclusion: "Tong Guan Li Qiao" needling method combined with swallowing training can effectively improve cerebral microcirculation, enhance the motor function of swallowing muscles and promote the recovery of swallowing function in treating PSD patients.

As a pre-digestion method of breast milk microwave-digestion was adopted in this method, and the contents of calcium and phosphorus were determined by ICP-MS at the same time. By optimizing conditions of digestion and ICP-MS, appropriate internal elements and the isotope mass number and EPA200.8 interference calibration equation were chosen. The accuracy and reliability of method were verified through the recovery and milk powder analysis of national standard substances (GBW10017). Determination results are in the permissible range. The relative deviation (RSD) is less than 2.40%, while the recovery is between 102.8% and 104.0%. ICP-MS method has wide dynamic range, and doesn't need to dilute samples. It is suitable for lots of samples and multi-element analyzed at the same time, and making up default of different method and different element determined respectively in national standard. It is a determination method for calcium and phosphorus supply of breast milk.