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To investigate the effect of turmeric volatile oil (TVO) on the apoptosis and proliferation of human skin SCC A431 cells, A431 cells were incubated with different concentrations (5-80 mg•L⁻¹) of TVO in vitro．The proliferation and cell cycle were assessed by CCK8 assay. The change of morphology was observed with inverted microscope. Apoptosis was evaluated with AO/EB double staining and flow cytometry (FCM); cell cycle was analyzed with FCM ．Western blot method was used to detect caspase-3 and caspase-9 protein expression. Results indicated that TVO has significant inhibitory effects on the growth of A431 cells in a dose dependent relationship, the difference between groups has statistically significant (P<0.05). TVO group compared with control group, concentrations in cells shrivel and broken phenomenon, cell apoptosis rate increased, and a dose dependent and increased the expression of caspase-3 and caspase-9. The experiment results suggested that TVO could restrain skin squamous carcinoma A431 cells proliferation, and induce its apoptosis. The mechanism may be related to increase the expression of caspase-3 and caspase-9., Competing Interests: The authors of this article and the planning committee members and staff have no relevant financial relationships with commercial interests to disclose., (Copyright© by the Chinese Pharmaceutical Association.)

To find out the protein properties of the wild type and the rough type of Salmonella typhi (S. typhi); to probe the genetic mechanism of the rough variation of S. typhi. The proteins were separated from the wild type and the rough type of S. typhi, and then the samples were processed by 2D-PAGE and stained by Coomassie Brilliant R-250. The properties and relationship of the protein patterns were analyzed by the software Imagemaster 6.0. By the 2D-PAGE, the similar protein patterns with 78% Sd (similar data) would be found between the wild type and the rough type of S. typhi. The distribution of most protein spots was in the range of pH 3.0 - 6.4 where the molecular weight of protein was smaller than 30kDa. According to the characters of differential proteins, there is not only the polysaccharide side chain of the O antigen but also some proteins that could be lost or changed in the rough strain of S. typhi. Most proteins in it would be the same as those in the parental bacteria so that the characteristic protein pattern of Salmonella can be found by 2D-PAGE. This property is useful for analysis and identification of the homologue between variations and their parental bacteria.