1. Research on optimization of the extraction process and antioxidant activity of anthocyanins from Lycium ruthenicum Murr.
- Author
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XUE Hai-Rui, LI Guo-Xiu, SUN Yi-Ran, WANG Tao-Yu, FAN Yun-Fang, and TANG Lin
- Abstract
To investigate the optimal extraction process and antioxidant activity of anthocyanins of Lycium ruthenicum Murr., the anthocyanins of Lycium ruthenicum Murr. were extracted by ultrasonic enzyme-assisted extraction, ethanol/ammonium sulfate aqueous two-phase extraction, ethanol/sodium dihydrogen phosphate aqueous two-phase extraction, and the extraction process was optimized by response surface methodology, the antioxidant model of BRL 3A cells induced by t-BHP was used to explore the protective effect of Lycium ruthenicum Murr. anthocyanins on oxidative stress. The results showed that the yield of ultrasonic enzyme assisted extraction was (17.92 ± 0.04) mg/g; the yield of ethanol/ammonium sulfate aqueous two-phase extraction was (15.46 ± 0.31) mg/g; the extraction yield of ethanol/ sodium dihydrogen phosphate aqueous two-phase system was (15.02 ± 0.19) mg/g. The in vitro antioxidant experiments showed that the antioxidant activity of anthocyanins (LRN) extracted by EtOH/ (NH
4 )2 SO4 aqueous two-phase extraction was the strongest, purified EtOH/(NH4 )2 SO4 aqueous two-phase extraction of anthocyanins (LRNA) can alleviate t-BHP-induced oxidative damage by reducing ROS production in cells. In summary, this process is convenient and stable and can be used for the extraction of anthocyanins from Lycium ruthenicum Murr. At the same time, it also proves that LRN has the strongest antioxidant activity. [ABSTRACT FROM AUTHOR]- Published
- 2023
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